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1991 Fiscal Year Final Research Report Summary

Changes in functions of intermediate filaments in alzhaimer cell

Research Project

Project/Area Number 02670515
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionOsaka University

Principal Investigator

TAKEDA Masatoshi  Osaka University Medical School Assistant Professor, 医学部, 講師 (00179649)

Co-Investigator(Kenkyū-buntansha) TADA Kunitoshi  Osaka University Medical School Assistant Professor, 医学部, 講師 (80135681)
HARIGUCHI Shiro  Osaka University Medical School Associate Professor, 医学部, 助教授 (10028459)
NISHIMURA Tsuyoshi  Osaka University Medical School Professor, 医学部, 教授 (70028455)
Project Period (FY) 1990 – 1991
KeywordsAlzheimer's Disease / fibroblast / vimentin / fodrin / intermediate Filament / calcium / protease / ubiquitin
Research Abstract

The fibroblasts were cultured from the cutaneous tissue of patients with familial Alzheimer's disease and non-familial Alzheimer's disease. In the process of collecting many clones of fibroblasts, a clone with a mutation in amyloid beta-protein precursor gene was found. The mutation was the same as reported by Goat et al, i. e. a substitution of Val to Ile at 717 of amyloid beta-protein precursor. The distribution of vimentin fibers in the cultured fibroblasts were studied, showing the drastic derangement in vimentin distribution in Alzheimer cells. The derangement was most obviously noticed with the cells with the mutation. The vimentin molecule showed no changes but the fodrin showed the increased accumulation in fibroblasts from Alzheimer patients. It was found that fodrin degradation in Alzheimr cells is inhibited. Considering the possibility that fodrin is degraded by Calcium-activated neutral protease (CANP), the calcium level and mobilization in Alzheimer cells was investigated. The results showed that calcium mobilization is significantly suppressed in Alzheimer fibroblasts. It is highly speculated that metabolism of cytoskeletal proteins such as fodrin is altered in Alzheimer cells which is closely related with the pathological processes observed in Alzheimer brain.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] M.Takeda,T.Nishimura,T.Kudo,S.Tanimukai,K.Tada: "Buffy coat from families of Alzheimer's disease patients produces intracytoplasmic neurofilament accumulation in hamster brain" Brain Research. 551. 319-321 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.takeda,T.Nishimura,T.Kudo,S.Tanimukai,T.Gotow,H.Tanabe,T.Miki,Y.Nakamura,H.Niigawa,H.Morita,T.Tanaka,Y.Tatebayashi,K.Tada: "Characterization of intracytoplasmic neurofilament accumulation in hamster brain caused by Alzheimer buffy coat inoculation-in comparison with experimental neurofibrillary changes produced by aluminum intoxication-" Gerontology. 37(suppl 1). 31-42 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Takeda,T.Nishimura,K.Tada,T.Kudo,S.Tanimukai: "Inoculation study of Alzheimer buffy coat into hamster brain--mmunohistochemical characterization of intracytoplasmic neurofilament accumulation in neurons of the lower brain stem nuclei-" Dementia. 2. 57-63 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Nakamura,M.Takeda,H.Suzuki,H.Hattori,K.Tada,S.Hariguchi,S.Hashimoto,T.Nishimura: "Abnormal distribution of cathepsins in the brain of patients with Alzheimer's disease" Neuroscience Letters. 130. 195-198 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Takeda,Y.Tatebayashi,S.Tanimukai,Y.Nakamura,T.Tanaka,T.Nishimura: "Immunohistological Study of Microtubule-associated Protein 2 and Ubiquitin in Chronically Aluminum-intoxicated Rabbit brain" Acta Neuropathologica. 82. 346-352 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Takeda,T.Nishimura,Y.Tatebayashi,T.Tanaka,S.Tanimukai,K.Tada: "Study of cytoskeletal proteins in cultured fibroblast of Alzheimer's disease" Acta Neurologica Scandinavica. 84. 416-420 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 西村 健,武田 雅俊ほか: "国際基礎神経科学ワ-クショップ" ライフサイエンス出版, 50 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Takeda, T. Nishimura, T. Kudo, S. Tanimukai, K. Tada: "Buffy coat from families of Alzheimer's disease patients produces intracytoplasmic neurofilament accumulation in hamster brain" Brain Research. 551. 319-321 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takeda, T. Nishimura, T. Kudo, S. Tanimukai, T. Gotow, H. Tanabe, T. Miki, Y. Nakamura, H. Niigawa, H. Morita, T. Tanaka, Y. Tatebayashi: "Characterization of intracytoplasmic neurofilament accumulation in hamster brain caused by Alzheimer buffy coat inoculation -in comparison with experimental neurofibrillary changes produced by aluminum intoxication-" Gerontology. 37(suppl 1). 31-42 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takeda, T. Nishimura, K. Tada, T. Kudo, S. Tanimukai: "Inoculation study of Alzheimer buffy coat into hamster brain -immunohistochemical characterization of intracytoplasmic neurofilament accumulation in neurons of the lower brain stem nuclei-" Dementia. 2. 57-63 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Nakamura, M. Takeda, H. Suzuki, H. Hattori, K. Tada, S. Hariguchi, S. Hashimoto, T. Nishimura: "Abnormal distribution of cathepsins in the brain of patients with Alzheimer's disease" Neuroscience Letters. 130. 195-198 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takeda, Y. Tatebayashi, S. Tanimukai, Y. Nakamura, T. Tanaka, T. Nishimura: "Immunohistological Study of Microtubule-associated Protein 2 and Ubiquitin in Chronicaly Aluminum-intoxicated Rabbit brain" Acta Neuropathologica. 82. 346-352 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takeda, T. Nishimura, Y. Tatebayashi, T. Tanaka, S. Tanimukai, K. Tada: "Study of cytoskeletal proteins in cultured fibroblast of Alzheimer's disease" Acta Neurologica Scandinavica. 84. 416-420 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Yoshimi, M. Takeda, T. Nishimura, T. Kudo, Y. Nakamura, K. Tada, N. Iwata: "An immunohistochemical study of MAP2 and clathrin in gerbil hippocampus after cerebral ischemia" Brain Research. 560. 149-158 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Nakamura, M. Takeda, K. J. Angelides, K. Tada, S. Hariguchi, T. Nichimura: "Assembly, disassembly, and exchange of glial fibrillary acidic protein" CLIA. 4. 101-110 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Nakamura, M. Takeda, Y. Kato, T. Tanaka, J. Tanaka, K. Nishinuma, T. Nishimura: "Glial Fibrillary Acidic Protein stimulates Lymphocyte proliferation and immunoglobulin synthesis of Alzheimer's disease patients" Meth and Find Exp Clin Pharmacol. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takeda, T. Nishimura, T. Kudo, S. Tanimukai, T. Tatebayashi, M. Okochi, A. Sekiyama, T. Kurumadani, S. Hariguchi: "Transmission study of neurofilament pathology in hamster brain produced by Alzheimer buffy coat inoculation" The Treatment of Dementia : A New Generation of Progress. Plenum Press. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takeda, T. Nishimura: "Changes in cytoskeletal system in fibroblasts from patients with familial Alzheimer's disease" Prog Neuropsychopharmacol & Biol Psychiat. (1991)

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      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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