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1991 Fiscal Year Final Research Report Summary

Injury to plasma cell membrane after ischemia or hemorrhagic shock

Research Project

Project/Area Number 02670543
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionOsaka University

Principal Investigator

YOKOTA Junichiro  Osaka University Medical School, Assistant, 医学部, 助手 (10166879)

Co-Investigator(Kenkyū-buntansha) YOSHIOKA Toshiharu  Osaka University Medical School, Associate Professor, 医学部, 助教授 (60127313)
Project Period (FY) 1990 – 1991
KeywordsFree radical / Superoxide dismutase / Catalase / Iron chelator / Endotoxin / カタラーゼ / 鉄キレート剤 / エンドトキシン
Research Abstract

We have previously reported that leukopenia or a combined treatment with superoxide dismutase (SOD) and catalase (CAT) can attenuate changes of membrane potential in postischemic skeletal muscle, and that free radical scavengers can diminish the persistent depolarization of cardiac and skeletal muscle cells following resuscitation from hemorrhagic shock.
In the first experiment of the current study, the effect of oxygen free radical scavengers (SOD+CAT) on blood flow, ATP content, and plasma cell membrane potential in the liver was evaluated after resuscitation from hemorrhagic shock. Oxygen free radical (OFR) -mediated cell membrane alteration was not noted in the lever, in contrast to cardiac and skeletal muscle. Pretreatment with scavengers, however, significantly improved portal vein blood flow and intestinal vessel blood flow. The results suggested that OFR-mediated alteration of the hepato-splanchnic circulation play an important role after hemorrhagic shock. In the second experiment, therefore, blood endotoxin was determined in the ischemia-reperfusion state of intestine. Significant endotoxemia was noted after declamping superior mesenteric artery. This study paved the way for the future study on bacterial translocation in the shock state. In the third experiment, to evaluate whether iron chelation with deferoxamine may interrupt postischemic skeletal muscle cell injury, tissue thiobarbituric acid reactive substances and membrane potential were measured during hind limb ischemia and after reperfusion. Pretreatment with iron chelator prevented lipid peroxidation and improved persistent depolarization of skeletal muscle cells.
Consequently, these studies yielded two significant results. Iron chelator as well as OFR scavengers can improve muscle cell membrane dysfunction induced after ischemia or shock. Intestinal injury after ischemia or shock may be mediated by OFR, and may be related to bacterial translocation.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Junichiro Yokota: "Oxygen free radicals affect cardiac and skeletal cell membrane potential during hemorrhagic shock in rats." American Journal of Physiology. 262. H84-H90 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junichiro Yokota: "Effect of oxygen free radical scavengers on blood flow regulation and cellular function in liver during hemorrhagic shock." Circulatory Shock.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junichiro Yokota: "Deferoxamine Prevents lipid peroxidation and attenuates reoxygenation injury in postischemic skeletal muscle." Journal of Trauma.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横田 順一朗: "虚血障害とフリーラヂカル." 救急医学. 14. 189-197 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横田 順一朗: "虚血と臓器障害(宮崎 正夫編)" へるす出版, 142 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junichiro Yokota, et al.: "Oxygen free radicals affect and skeletal cell membrane potential during hemorrhagic shock in rats." American Journal of Physiology. 262. 84-90 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junichiro Yokota, et al.: "Effect of oxygen free radical scavengers on blood flow regulation and cellular function in liver during hemorrhagic shock."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junichiro Yokota, et al.: "Deferoxamine prevents lipid peroxidation and attenuates reoxygenation injury in postischemic skeletal muscle."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junichiro Yokota: "Ischemic injury and free radical" Japanese Journal of Acute Medicine. 14. 189-197 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junichiro Yokota, et al.: "Ischemia-reperfusion injury and oxygen free radicals. In Ischemia and Organ Failure ed. by Masao Miyazaki" Herusu-Syuppan Co. Ltd., Tokyo. 111-122 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-18  

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