1992 Fiscal Year Final Research Report Summary
Experimental study of hypoplastic lungs with surgical disease in fetus.
| Project/Area Number |
02670554
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
TAKAMATSU Hideo Kagoshima University, Faculty of medicine, Professor, 医学部, 教授 (50142427)
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| Co-Investigator(Kenkyū-buntansha) |
TAHARA Hiroyuki Kagoshima University, Faculty of medicine, Research associate, 医学部, 助手 (70236719)
NOGUCHI Hiroyuki Kagoshima University, Faculty of medicine, Assistant professor, 医学部, 講師 (80198580)
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| Project Period (FY) |
1990 – 1992
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| Keywords | Hypoplastic lung / Congenital diaphragmatic hernia / Nitrofen / Tenascin |
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| Research Abstract |
We tried to make an experimental animal model for the investigation of fetal hypoplastic lungs with surgical disease.
It has been well known that Nitrofen(2, 4-dichloro-4 nitrodiphenylether) induces in high frequency various types of CDH(congenital diaphragmatic hernia) and hypoplastic lungs in mice and rats.
One hundred mg or two hundred and fifty mg per kg bodyweight of Nitrofen with 3-5ml olive oil was administered to pregnant rat by intragastric intubation on 9th or 11th day of gestation. A dam was sacrificed on the 20th of gestation and fetuses were removed from a dam. The number of animals with diaphragmatic defect, position(right- or left-side), intrathoracic contents were evaluated. And hypoplastic lungs were studied immunohistochemically with Tenascin which is an extracelluar matrix glycoprotein playing a vital role in pathological change (embryogenesis, certain sequential events such as cell death, proliferation, attachment, detachment, migration, morphogenesis and cytodifferentiation).
Administration of 250mg per kg bodyweight of Nitrofen on 9th day of gestation induced diaphragmatic defect most frequently.
Immunohistochemically, expression of Tenascin was not observed in hypoplasic lung nor normal lung.
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