Basic Study on transplantation of Langerhans islets into the liver via hepatic artery in animals.

1991 Fiscal Year Final Research Report Summary

• Project/Area Number: 02670591
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: The Jikei University School of Medicine.
• Principal Investigator: KOBAYASHI Susumu The Jikei University School of Medicine, Department of Surgery (1), Lecturer, 医学部, 講師 (20147309)
• Co-Investigator(Kenkyū-buntansha): YAMADERA Hitoshi The Jikei University School of Medicine, Department of Surgery (1), Assistant, 医学部・第一外科学教室, 助手 (40220420) ; NAKABAYASHI Yukio The Jikei University School of Medicine, Department of Surgery (1), Assistant, 医学部・第一外科学教室, 助手 (70217818) ; MATSUDA Minoru The Jikei University School of Medicine, Department of Surgery (1), Assistant, 医学部・第一外科学教室, 助手 (30186407) ; UNEMURA Yasuki The Jikei University School of Medicine, Department of Surgery (1), Assistant, 医学部・第一外科学教室, 助手 (20185820) ; INADA Shouzou The Jikei University School of Medicine, Department of Surgery (1), Assistant, 医学部・第一外科学教室, 助手 (30168405)
• Project Period (FY): 1990 – 1991
• Keywords: rat / pancreatic islets / hepatic artery / transplantation

Research Abstract

At the beginning of the experiment, an experimental model was prepared by adult hybrid dogs but liberation of pancreatic cells of dogs was difficult making it impossible to continue the experiment. Therefore, the experimental animals were changed from hybrid dogs to SD strain male rats. Pancreatic cells of rats were relatively easily liberated and many free cells were able to be recovered. However, the hepatic artery of a rat was too fine to permit planned infusion of free pancreatic cells and therefore it took time to establish the technique. Free pancreatic colls were successfully infused into the liver by infusing them into the gastroduodenal artery after cramping the common hepatic artery and gastroduodenal artery. It was also successful to visually confirm the pancreatic cells had survived. The confirm this, diabetic rats were used. Diabetic rats were prepared by intravenous injection of streptozotocin.
It will be necessary, after this, to confirm the survival of transplanted pancreatic cells using diabetic rats.
Besides that, the end to side anastomosis of the portal vein and inferior vena cava and transposition of the portal vein and inferior vena cava were also successful in an animal model.
After this, it will be necessary to transplant free pancreatic cells into the liver via the hepatic artery using the planned animal model (end to side anastomosis of the portal vein and inferior vena cava and transposition of the portal vein and inferior vena cava) in order to demonstrate the effect of the hepatotrophic factor secreted from pancreatic cells as a regenerative factor of the liver.