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1992 Fiscal Year Final Research Report Summary

Disturbance of cholinergic pathway after cerebral hemorrhage and its therapeutic strtategy

Research Project

Project/Area Number 02670641
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionKinki University

Principal Investigator

KATAOKA Kazuo  Kinki University school of medicine, Associate professor, 医学部, 講師 (10221178)

Co-Investigator(Kenkyū-buntansha) IOKU Masahiko  Kinki University school of medicine, Professor, 医学部, 教授 (80088541)
KURODA Ryotaro  Kinki University school of medicine, Associate professor, 医学部, 助教授 (10161803)
ASAI Toshiharu  Kinki University school of medicine, Assistant professor, 医学部, 助手 (20248008)
TOKUNO Tatsuya  Kinki University school of medicine, Assistant professor, 医学部, 助手 (00248006)
Project Period (FY) 1990 – 1992
KeywordsCerebral hemorrhage / Subcortical infarction / Cholinergic pathway / Acetylcholinesterase / Nucleus Basalis Meynert / Neuronal fiber
Research Abstract

Purpose : We studied the changes in the cholinergic system after experimental subcortical infarction and subcortical mass lesion to understand neuronal network disturbances following subcortical stroke (cerebral hemorrhage or subcortical infarction). Methods : We quantitatively evaluated the highly sensitive acetylcholinesterase (AChE) histochemistry 7 days, 3 months, and 12 months after subcortical infarction or subcortical mass lesion in rats. Subcortical infarction was induced by a single middle cerebral artery (MCA) occlusion. Subcortical mass lesion was induced by insertion and inflation of microballoon. Immunohistochemistry was also studied to determine the changes in cholinergic neurons, glial response, and extent of brain edema. We evaluated neurofunctional changes after subcortical lesion by [^<14>C] 2-deoxyglucose autoradiography. Deoxyglucose autoradiography was performed during physiological stimulation of the right face or without specific stimulation. Results : In sham operated rats, quantitative evaluation of AChE histochemistry showed approximately 10m/mm^3 frontal cortex AChE positive fibers. Subcortical lesion resulted in significant loss of AChE positive fibers in the ipsilateral cortex. One week after subcortical lesion, AChE positive fiber became 2 m/mm^3 frontal cortex. Three months after subcortical lesion, AChE positive fiber slightly recovered to 3 m/mm^3 frontal cortex. We could not find further recovery 12 months after subcortical lesion. Deoxyglucose autoradiography during stimulation showed disturbances of cortical activation 1 week after subcortical lesion. Three months after subcortical lesion, the activation of somatosensory cortex was recovered. Comments : Present study morphologically revealed neurofunctional disturbances caused by subcortical lesion. We noted a tendency of recovery of function as well as fiber innervation in the chronic stage.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 早川 徹: "脳出血,クモ膜下出血の実験モデル." 神経研究の進歩. 35. 83-94 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 片岡 和夫: "Cholinergic deafferentation after focal cerebral infarct in rats." Stroke. 22. 1291-1296 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 片岡 和夫: "Changes in somatosensory circuits after subcortical infarct in rats." Restorative Neurology and Neuroscience. 4. 323-330 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 徳野 達也: "Functional changes in thalamic relay neurons after focal cerebral infarct." Journal of Cerebral Blood Flow and Metabolism. 12. 954-961 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayakawa T, Kataoka K: "Experimental models of cerebral hemorrhage and subarachnoid hemorrhage." Shinkei Kenkyu no Shimpo (Japanese). 35. 83-94 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka K, Hayakawa T, Kuroda R, Yuguchi T, Yamada K: "Cholinergic differentation after focal cerebral infarct in rats" Stroke 22. 1291-1296 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka K, Hayakawa T, Mushiroi T, Wakayama A, Yamada K, Kuroda R, Ioku M: "Changes in somatosensory circuits after subcortical infarct in rats." Restorative Neurology and Neuroscience. 4. 323-330 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokuno T, Kataoka K, Asai T, Chichibu S, Kurada R, Itoku M, Yamada K, Hayakawa T: "Functional changes in thalamic relay neurons after focal cerebral infarct. A study of unit recordings from VPL neurons after MCA occlusion in rats" Journal of Cerebral Blood Flow and Metabolism. 12. 954-961 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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