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1992 Fiscal Year Final Research Report Summary

Study on lipid Reroxidation by halothane and its inhibition

Research Project

Project/Area Number 02670682
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

FUJII Kohyu  Hiroshima Univ. School of Med. Assistant Professor, 医学部, 講師 (60034021)

Co-Investigator(Kenkyū-buntansha) YUGE Osafumi  Hiroshima.Univ. School of Ined. Professor, 医学部, 教授 (40034128)
MORIO Michio  Chugoku Rosai Hospital Rear, 院長 (80033950)
Project Period (FY) 1990 – 1992
KeywordsHepatotoxicity / Halothane / Lipid peroxidation / Reduction
Research Abstract

Formation of metabolites of halothane under anaerobic or aerobic cobdition by the reaction mixture of liver microsomes and NADPH was examined using rats, guinea pigs or rabbits. 1.Formation of metabolites of halothane, trifluoroacetic acid and anaerobic metabolites, 2-chlor,1,1,1-trifluoroethane and 2-chloro-1,1-difluoroethylene, were inhibited 50% by 4.96mM and 35.3mM paraquat, respectively. Apossoble mechanism was speculated for the inhibitory effects; one is the impaird formation of halothane-cytochrome p450 complex by the addition of paraquat, and the other is the diversion of electron from cytochrome p450 reductase to generate active paraquat radicals. 2.By in vivo treatment, halothane anaerobic dehalogenation was induced by phenobarbital, thiopental, thiamylal, pentobarbital and secobarbital. 3.The reductive metabolism of halothane was reduced by calcium blocking agents, verapamil, diltiazem, nicardipine and nifedipine. NADPH-cytochrome p450 reductase activity in microsomes did not change by the addition of these drugs. 4.The reductive metabolism of halothane was reduced by analgesic or hyponotic agents, fentanyl, morphine, pentazocine, buprenorphine, ketamine, diazepam, chlorpromazine, and hydroxyzine. NADPH-cytochrome p450 reductase activity in microsomes did not change by the addition of these drugs. 5.The reductive metabolism of halothane was enhanced at 0.3% of isoflurane or 0.5-0.8% of sevoflurane. Higher concentration than 1% of these anesthetics, the reductive metabolism of halothane was inhibited. 6.Elevated serum transaminasen (GOT GPT) and TBA reactive substance by in vivo inhaled halothane to guinea pig were inhibited by the adminstration of Vitamin E. 7.The reductive metabolism of halothane was reduced by Gomisi, driedripe fruit of schizandra chinesis Ball which is medical herb. NADPH-cytochrome p450 reductase activity in microsomes did not change by the addition of this drugs.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] SATO,N.,FUJII,K.: "The association of halothane-induced lipid peroxidation with te anarobic meabolism of halothane:An in vitro study in guinea pig liver microsomes" Hiroshma J.Med.Sci.39. 1-6 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河本 昌志,藤井 宏融: "パラコートはハロセン代謝産物の生成に影響するか、" 麻酔と蘇生. 26(S). 69-72 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TAIRA,Y.,FUJII,K.: "Effects of in vivo pretratment with various barbiturates on anaerobic halothane metabolism in rat liver microsomes." Hiroshima J.Medical Sciences. 39. 125-130 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KAWAHARA,M.,AKITA,S.,FUJII,K.: "Effect of calcium channel blocking agents on the reductive metabolism of halothane." Jounal of Applied Toxicology. 11. 29-31 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] YAMANOUE,T.,KIKUCHI,H.,FUJII,K.: "Inhibition by analgesic and hypnotic agents on anaerobic dehalogenation of halothane." J.Anesthesia. 5. 331-337 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤井 宏融,肥後 正徳: "イソフルレンとセボフルレンはハロセンの嫌気的脱ハロゲン化反応に影響をおよぼすか:" 麻酔と蘇生. 27(S). 1-2 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SATO N,FUJII K,YUGE O,MORIO M: "Change in lipid peroxi-dation levels and lipid composition in the lungs,livers,kidneys and brains of mice treated with paraquat." J.Applied Toxicology. 12. 365-368 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ni Jiaxiang,Kohyu FUJII: "Inhibitory effect of Gomisi on reductive metabolism of halothane:" Hiroshima J Anesthesia. 13(S). 57-60 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuyoshi Sato, Kohyu Fujii, Osafumi Yuge, Michio Morio: "The Association of Halothane-Induced Lipid Peroxidation with the Anaerobic Metabolism of Halothane: An In Vitro Study in Guinea Pig Liver Microsomes." Hiroshima J. Medical Sciences. 39. 1-6 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masasi Kawamoto, Kohyu Fujii, Osafumi Yuge, Michio Kawahara, Michio Morio: "Interaction between Paraquat and Halothane Metabolism" Hiroshima J.Anesth. 26(s). 69-71 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yutaka Taira, Kohyu Fujii, Hirosato Kikuchi, Osafumi Yuge, Michio Morio: "Effects of in vivo pretreatment with vaious barbiturates on anaerobic halothane metabolism in rat liver microsomes" Hiroshima J.Medical Sciences. 39. 125-130 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Michio Kawahara, Susumu Akita, Kohyu Fujii, Michi Morio: "Effects of calcium channel blocking agents on the reductive metabolism of halothane" J.Applied Toxicology. 11. 29-31 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takao Yamanoue, Hirosato Kikuchi, Kohyu Fujii, Osafumi Yuge, Michio Kawahara, Michio Morio: J.Anesthesia. 5. 331-337 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohyu Fujii, Masanori Higo, Nobuyoshi Sato, Michi Kawahara, Michio Morio: "Isoflurane or Sevoflurane enhance halothane anaerobic dehalogenation in vitro" 27(s). 1-2 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyoshi Sato, Kohyu Fujii, Osafumi Yuge, Michio Morio: "Effects of Vitamin E on Halothane-induced Lipid Peroxidation and Liver Injury" Hiroshima J.Anesth. 27(s). 83-95 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyoshi Sato, Kohyu Fujii, Osafumi Yuge Michio Morio: "Change in lipid peroxidation levels and lipid composition in the lungs, livers, kidneys and brains of mice treated with paraquat." J.Applied Toxicology. 12. 365-368 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ni Jiaxiang, Kohyu Fujii, Nobuyoshi Sato, Osafumi Yuge: "Inhibitory effect of Gomisi on reductive metabolism of halothane." Hiroshima J.Anesth. 28(s). 57-60 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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