1991 Fiscal Year Final Research Report Summary
Mutagenicity in human bladder cancer cell line exposed to hematoporphyrin derivative photoradiation and ultraviolet Radiation
| Project/Area Number |
02670701
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Yamaguchi University School of Medicine (1991)
Kanazawa University (1990) |
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Principal Investigator |
NAITO Katsusuke Yamaguchi University School of Medicine Urology, Prop., 医学部, 教授 (60115251)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUTAMA Hideyasu Yamaguchi University Hospital Urology research Associate, 医学部附属病院, 助手 (70209667)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Photodynamic action / Mutagencity / Tissue culture / Hematoporphyrin derivative / UVB / Argon-dye laser / アルゴン色素レ-ザ- |
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| Research Abstract |
Cell culture studies have been performed to compare the mutagenic potential for hematoporphyrin derivative photoradiation and UV radiation. The mutation frequency in human bladder cancer cells (KK-47) at the hypoxanthine-guanine phosphoribosyltransferase locus was measured using resistance to 6-thioguanine. Treatment with UVB (wavelength ; 280-320 nm) was effective in producing mutants resistant to 6-thioguanine, but treatment with neither UVA (wavelength ; 300-430 nm) nor hematoporphirin derivative photoradiation (at comparable toxicity levels) using argon-dye laser (wavelength ; 630 nm) did not induce any mutagenic activity above background levels. Additional studies regarding hernatoporphyrin derivative UV radiation are needed. And in vivo carcinogenic studies will have to be performed to determine the long-term effects of hematoporphyrin derivative photoradiaton.
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