Flow cytometry analysis of rejection and infection with monoclonal antibody in renal transplant recipients

1991 Fiscal Year Final Research Report Summary

• Project/Area Number: 02670706
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Osaka University
• Principal Investigator: KOKADO Yukito Osaka Univ. Depat. of Urology Assist Prof., 医学部, 助手 (30186639)
• Co-Investigator(Kenkyū-buntansha): TAKAHARA Shiro Osaka Univ. Depat. of Urology Assist Prof., 医学部, 助手 (70179547) ; ISHIBASHI Michio Osaka Univ. Depat. of Urology Assoc Prof., 医学部, 助教授 (40107032)
• Project Period (FY): 1990 – 1991
• Keywords: subset / rejection / infection / cytomegarovirus / OKT4 / OKT8 / T8-FITC / S6F-RD1

Research Abstract

Purpose : To diagnosis rejection or infection in renal transplant recipients, we studied the subset analysis of T cell in periferal blood mononuclear cells using monoclonal antibodies.
Methods and Results : (1)To evaluate recipient's lymphocytes response, recipient's lymphocyte were cultured with recipient's HCMV infected fibroblast or donor's HCMV infected fibroblast. There were no significance responses.
(2)We studied the subset analysis of cytotoxic effector T cell in peripheral blood mononuclear cells using a new monoclonal antibody T8-FITC/S6Fl-ROl in renal transplant recipients. In a half of patients who had acute rejections, the increase of T8+S6Fl+ cells was identified. These cells positively appeared at the onset of the infection episodes. But we could not distinguish acute rejection from infection.
(3)OKT4/OKT8 ratios were decreased when CMV was isolated from the urine or blood. If OKT4/OKT8 ratios in recipients peripheral blood mononuclear cells is decrease, renal dysfunction may be due to-infection.

Research Products

• [Publications] 高野 右嗣,小角 幸人ほか: "腎移植後,拒絶反応および感染症に対するmonoclonal antibody T8ーFITC/S6F1ーRD1によるtowーcolorーflow cytometry解析" 西日本泌尿器科. 54. (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.Kokado,et al.: "Clinical significance of positive cytomegarovirus cultures from virine and/or blood" Transplant.Proceeding. (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takno, Y. Kokado, Y. Takahara, S. Kameoka, H. Ishibashi, M. Okuyama, A.: "Two-color flow cytometry analysis of rejection and infection with monoclonal antibody T8-FITC/S6F1-RD1 in renal transplant recipients" Nishinihon Journal of Urology. (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kokado, Y. Takahara, S. Takano, Y. Kameoka, H. Ishibashi, M. Tanaka, K. Oosumi, Y. Okaba, S. Okuyama, A. Sonoda, T.: "Clinical significance of positive cytomegarovirus cultures from urine and/or blood" Transplant. Proc.(1992)
  • Description: 「研究成果報告書概要(欧文)」より