1992 Fiscal Year Final Research Report Summary
Effects of Growth Factors on Hormone Release and Intracellular Ca^<2+> of granulosa cells
| Project/Area Number |
02670735
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KAMADA Shusaku Tokyo Medical and Dental Univ. Research associate, 医学部, 助手 (80169606)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Toshiro Tokyo Medical and Dental Univ. Lecturer, 医学部, 講師 (50126223)
ASO Takeshi Tokyo Medical and Dental Univ. Professor, 医学部, 教授 (60093176)
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| Project Period (FY) |
1990 – 1992
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| Keywords | IGF-II / IGF-I / granulosa cell / Steroid hormone / Cell proliferation / cyclic AMP / intracellular Ca^<2+> / Autocrine control |
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| Research Abstract |
The effects of insulin-like growth factor-II (IGF-II) on the proliferation and differentiation of ovarian granulosa cells were studied in vitro. IGF-II significantly increased basal progesterone secretion in granulosa cells at concentrations of 1 - 100 ng/ml. A stimulatory effect was also observed in gonadotropin-stimulated porcine granulosa cells treated with IGF-II. The secretion of estradiol by basal and gonadotropin-stimulated porcine granulosa cells was also significantly increased by adding IGF-II. IGF-II led to dose-dependent increases in [^3H]thymidine incorporation into DNA. Treatment with IGF-II produced a significant increase in the basal accumulation of cyclic AMP in porcine granulosa cells. However, no change of intracellular Ca^<2+> concentration by IGF-II was noted. IGF-II produced effects in accumulation that were similar to those of IGF-I. Our findings suggest that IGF-II may be a general stimulation in the proliferation and differentiation of granulosa cells, and that cyclic AMP may be a second messenger for these effects of IGF-II.
Moreover, IGF-II concentrations were measured using an RIA technique in human follicular fluid (FF) obtained from 20 patients who were undergoing IVF and ET. The large quantity of IGF-II in FF was observed and significant positive correlations were observed between IGFs (IGF-I and IGF-II) and sex steroid hormones. These findings suggest IGFs in FF may play possibly some role in ovarian steroidogenesis.
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[Publications] Kamada,S., Kubota,T., Taguchi,T., Wen-Rong,H.Sakamoto,S., Aso,T.: "Effects of insulin-like growth factor-II on proliferation and differentiation of ovarian granulosa cells." Hormone Research. 37. 141-149 (1992)
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[Publications] Kubota,T., Kamada,S., Ohara,M., Taguchi,M., Sakamoto,S., Shimizu,Y., Aso,T.: "Insulin-like growth factor II in follicular fluid of the patients with in vitro fertilization and embryo transfer." Fertility and Sterility. (1993)
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[Publications] Kubota,T., Kamada,S., Ohara,M., Koi,H., Sen,K., Aso,T.: "Studies on insulin-like growth factor-II and IGF-I in human follicular fluids." Journal of Fertilization and Implantation. 8(1). 63-65 (1992)
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