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1992 Fiscal Year Final Research Report Summary

A human chromosome 7 carries genes with putative suppressor functions for choriocarcinoma and with differentiation inducing activities for mouse EC cells.

Research Project

Project/Area Number 02670747
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MIYAMOTO Singo  Medical Institute of Bioregulation Kyushu Univ. ASSISTANT, 生体防御医学研究所, 助手 (40209945)

Co-Investigator(Kenkyū-buntansha) WAKE Norio  Medical Institute of Bioregulation Kyushu Univ. PROFESSOR, 生体防御医学研究所, 教授 (50158606)
IMAMURA Toshiro  Medical Institute of Bioregulation Kyushu Univ. ASSISTANT, 生体防御医学研究所, 助手 (10221095)
KATO Hidenori  Medical Institute of Bioregulation Kyushu Univ. ASSISTANT, 生体防御医学研究所, 助手 (60214392)
Project Period (FY) 1990 – 1992
Keywordschoriocarcinoma / Microcell fusion / Tumor suppressor genes / Chromosome #7 / EC cells / Differentiation / Endoderm
Research Abstract

In the present study, multiple chromosomes were independently transferred into CC1 choriocarcinoma cells via microcell fusion. Tumorigenic suppression as well as alterations in growth properties were observed in the microcell hybrids only following introduction of chromosome 7. These findings were compatible with the presence of a putative tumor suppressor gene(s) for this tumor cells on chromosome 7, suggesting the availability of a single chromosome transfer to disclose a chromosome carrying a gene whose expression is critical for cell differentiation. As a result of transfer of multiple human chromosomes into mouse F9 cells, introduction of a single human chromosome 7 could induce extinction of EC cell phenotypes as well as alterations in cell morphology and growth properties of F9 cells. The microcell hybrid containing a human chromosome 7 shared biochemical markers with endoderm cells, because expression of these markers was indistinguishable from that of F9 cells differentiated with retinoic acid treatment. These results suggested that a human chromosome 7 carried genetic informations which extinguish embryonal carcinoma functions and possibly caused the EC cell genome to express biochemical and immunological characteristics of endoderm.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] S.Miyamoto,N.Makino,H.Shimokawa,K.Akazawa,N.Wake,H.Nakano: "The characteristics of erythrocyte Na^+ transport systems in normal pregnancy and pregnancy-induced hypertension." Journal of Hypertension. 10. 367-372 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kato,J.Nishida,T.Honda,Y.Fujinaga,K.Fujinaga,N.Wake: "Chromosome alterations contributed to neoplastic progression of transformed rat embryonal fibroblasts." Cancer Genetics and Cytogenetics. 58. 39-47 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Imamura,T.Arima,H.Kato,S.Miyamoto,T.Sasazuki,N.Wake: "Chromosomal deletions and K-ras gene mutations in human endometrial carcinomas." International Journal of Cancer. 51. 47-52 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Honda,H.Kato,T.Gima,J.Nishida,M.Sasaki,T.Imamura,N.Wake: "Involvement of p53 gene mutations in human endometrial carcinomas." International Journal of Cancer. 54. 1-5 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Sasaki,H.Yamada,Barrett,J.C.Wake,N,Oshimura,M: "Normal human chromosome 7 carries a putative suppressor gene(s) for a human choriocarcinoma cell line." Oncogene,. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato,H.: "Chromosome alterations contributed to neoplastic progression of transformed rat embryonal fibroblasts." Cancer Genetics and Cytogenetics.58. 39-47 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto,S.: "The characteristics of erythrocyte Na+ transport systems in normal pregnancy and pregnancy-induced hypertension." Journal of Hypertension.10. 367-372 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imamura,T.: "Chromosomal deletions and K-ras gene mutations in human endometrial carcinomas." International Journal of Cancer.51. 47-52 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda,T.: "Involvement of p53 gene mutation in human endometrial carcinomas." International Journal of Cancer.54. 1-5 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki,M.: "Normal human chromosome 7 carries a putative suppressor gene(s) for a human choriocarcinoma cell line." Oncogene. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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