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1991 Fiscal Year Final Research Report Summary

Regulation of Cytokines for Gene Expression and Function of Gingival Fibroblasts

Research Project

Project/Area Number 02670812
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionMiekai University School of Dentistry

Principal Investigator

HANAZAWA Shigemasa  Meikai University School of Dentistry Assistant, professor, 歯学部, 助教授 (60060258)

Co-Investigator(Kenkyū-buntansha) AMANO Shigeru  Meikai University School of Dentistry Instructor, 歯学部, 講師 (90167958)
Project Period (FY) 1990 – 1991
KeywordsInflammation / Cytokines / Gingival fibroblasts / Periodonal pathogen / Periodontal disease / Gene expression
Research Abstract

Adherence to host cells is an essential stage in triggering the pathogenesis brought about pathogenic bacteria. Bacterial fimbriae may be an important cell structure for the adherence. In many cases, the adherence takes place through the binding of fimbriae to specific receptors on the host cell surface. Therefore, bacterial fimbriae play an important role in first stage of the pathogenesis caused by pathogenic bacteria.
Bacteroides (Porphyromonas) gingivalis, an oral anaerobic bacterium, has been recognized widely as a pathogenic organism in adult periodontitis which is a chronic inflammatory disease ; Chemical and immunological characterizations of -B. gingivalis fimbriae have been well demonstrated by Yoshimura et al. We suggested previously that the organism attaches to human fibroblasts through the binding of the fimbriae to specific receptors. Therefore, in the same fashion as adhesion of other gram-negative bacteria, B. gingivalis fimbriae-mediated adhesion is regarded as a first stage in pathogenesis of the microorganism infection.
In the present grant-aid for scientific research, we obtained the following results.
1) B. gingivalis fimbriae stimulated transcriptional activity of IL-1 beta in mouse peritoneal macrophages.
2) The fimbriae induced expression of neutrophil chemoattractant KC gene in mouse peritoneal macrophages via activation of protein kinase C.
3) The fimbriae induced IL-6 expression in human gingival fibroblasts, and the expression was mediated in part by membrane IL-1 alpha.
4) IL-1 and TNF induced IL-6 expression in human gingival fibroblasts.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] S.Hanazawa,Y.Murakami et al.: "Bacteroides(porphyromonas) gingivalis fimbriae activate mouse peritoneal macrophages and induce gene expression and production of interleukin-1." Infection and Immunity,. 59(6). 1972-1977 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 牛 忠英,花沢 重正,竹下 玲ら: "ヒト歯根膜由来の線維芽細胞の機能と遺伝子発現に関するtransforming growth factorーbetaの作用." 歯科基礎医学雑誌. 33. 133-140 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Takeshita,Zhon Gying Niu,S.Hanazawa et al: "Effect of interleukin-1 beta on gene expressions and functions of fibroblastic cells derived from human periodontal ligament." J.Periodontal Res.in Press. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] "Transforming growth factor-beta-induced gene expression of mocyte chemoattrant JE in mouse osteoblastic cells,MC3T3-E1." Bioche.Biophys.Res.Commun.180. 1130-1136 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Amano,S.Hanazawa,et al.: "An assay system utilizing bone for assessment of differentiation of osteoclast progenitors." J.Bone Mine.Res.7. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Hanazwa,Y.Murakami et al.: "Porphyromonas gingivalis fimbriae induce expression of the neutrophil chemotactic factorKC gene of mouse peritoneal macrophages:The role of protein kinase C." Infection and Immunnity. 60. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Hanazawa, Y. Murakami, K. Hirose, S. Amano, Y. Ohmori, H. Higuchi, and S. Kitano: "Bacteroides (porphyromonas) gingivalis fimbriae activate mouse peritoneal macrophages and induce gene expression and production of interleukin-1." Infection and Immunity. 59 (6). 1972-1977 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Takeshita, Zhon Gying Niu, S. Hanazawa, I. Takara, H. Higuchi, I. Katayama, and S. Kitano: "Effect of interleukin-1 beta on gene expressions and functions of fibroblastic cells derived from human periodontal ligament." J. Periodontal Res.(10). (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Hanazawa, A. Takeshita, Y. Tsukamoto, Y. Kawata, K. Ohta, I. Takara, and S. Kitano.: "Transforming growth factor-beta-induced gene expression of mocyte chemoattrant JE in mouse osteoblastic cells, MC3T3-E1." Bioche. Biophys. Res. Commun.180 (10). 1130-1136 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Amano, S. Hanazawa, Y. Kawata, K. Ohta, H. Kitami, and S. Kitano: "An assay system utilizing bone for assessment of differentiation of osteoclast progenitors." J. Bone Mine. Res.7 (3). (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Hanazawa, Y. Murakami, A. Takeshita, H. Kitami, K. Ohta, S. Amano, and S. Kitano.: "Porophyromonas gingivalis fimbriae induce expression of the neutrophil chemotactic factorKC gene of mouse peritoneal macrophages : The role of protein kinase C." Infect. Immun.60 (4). (1992)

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      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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