1991 Fiscal Year Final Research Report Summary
Regulation of Cytokines for Gene Expression and Function of Gingival Fibroblasts
| Project/Area Number |
02670812
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Miekai University School of Dentistry |
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Principal Investigator |
HANAZAWA Shigemasa Meikai University School of Dentistry Assistant, professor, 歯学部, 助教授 (60060258)
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| Co-Investigator(Kenkyū-buntansha) |
AMANO Shigeru Meikai University School of Dentistry Instructor, 歯学部, 講師 (90167958)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Inflammation / Cytokines / Gingival fibroblasts / Periodonal pathogen / Periodontal disease / Gene expression |
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| Research Abstract |
Adherence to host cells is an essential stage in triggering the pathogenesis brought about pathogenic bacteria. Bacterial fimbriae may be an important cell structure for the adherence. In many cases, the adherence takes place through the binding of fimbriae to specific receptors on the host cell surface. Therefore, bacterial fimbriae play an important role in first stage of the pathogenesis caused by pathogenic bacteria.
Bacteroides (Porphyromonas) gingivalis, an oral anaerobic bacterium, has been recognized widely as a pathogenic organism in adult periodontitis which is a chronic inflammatory disease ; Chemical and immunological characterizations of -B. gingivalis fimbriae have been well demonstrated by Yoshimura et al. We suggested previously that the organism attaches to human fibroblasts through the binding of the fimbriae to specific receptors. Therefore, in the same fashion as adhesion of other gram-negative bacteria, B. gingivalis fimbriae-mediated adhesion is regarded as a first stage in pathogenesis of the microorganism infection.
In the present grant-aid for scientific research, we obtained the following results.
1) B. gingivalis fimbriae stimulated transcriptional activity of IL-1 beta in mouse peritoneal macrophages.
2) The fimbriae induced expression of neutrophil chemoattractant KC gene in mouse peritoneal macrophages via activation of protein kinase C.
3) The fimbriae induced IL-6 expression in human gingival fibroblasts, and the expression was mediated in part by membrane IL-1 alpha.
4) IL-1 and TNF induced IL-6 expression in human gingival fibroblasts.
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