1992 Fiscal Year Final Research Report Summary
Development of Polyll-lacticdeacid microcapsule containing tetracycline for periodontal therapy
| Project/Area Number |
02670860
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Ohu University School of Dentistry |
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Principal Investigator |
MINABE Masato OHU UNIVERSITY Associate, 歯学部, 講師 (60148004)
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| Co-Investigator(Kenkyū-buntansha) |
IKADA Yoshito 京都大学, 生体医療工学研究センター, 教授 (00025909)
SAITO Kazuhiro 奥羽大学, 歯学部, 助手 (60225696)
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| Project Period (FY) |
1990 – 1992
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| Keywords | Periodontal therapy / Slow release derice / Minocycline / Lysozyme chloride / Atelocollagen pellet / Poly(L-lactic acid) microsphere |
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| Research Abstract |
In the present study, tetracycline(TC)-immobilized Poly(L-lactic acid) microspheres(TC-PLA-MS) were produced experimentally, and the slow release and clinical effects of TC administered in this form were examined both in vivo and in vitro. The results indicated that TC-PLA-MS was effective in maintaining TC concentration to some extent in periodontal pockets, but less effective for sustained release of the drug in the pocket. Basic and clinical studies were then performed to evaluate the combined administration of atelocollagen pellets containing immobilized minocycline(MINO) which was superior to TC in antimicrobial activity and stability(MINO-P) and lysozyme chloride-immobilized atelocollagen pellets(LYSO-P) produced experimentally to suppress inflammation, which was thought to be responsible for the diminished slow-release of drugs, in the pocket. The following results were obtained : (1)combined MINO-P and LYSO-P administration improved the concentration and sustained release of MINO in the pocket ; (2)LYSO-P was effective in improving inflammation in the pocket ; (3)MINO-P was effective in improving the bacterial condition in the pocket both qualitatively and quantitatively ; and (4)the effectiveness of combined administration of MINO-P and LYSO-P was confirmed in a comprehensive evaluation that included clinical, microbiological and biochemical examinations. It was also found that oral administration of MINO (200 mg/day for 2 weeks) and local application of MINO-P(twice at a one-week interval) were almost equally effective for clinical improvement. These results indicate the usefulness of periodontal therapy with combined use of MINO-P and LYSO-P.
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