1991 Fiscal Year Final Research Report Summary
Syntheses and Application of the Functional Compounds Designed to Study the Metabolism and the Mechanism of the Action of Vitamin D
| Project/Area Number |
02670946
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
YAMADA Sachiko Tokyo Med. and Dental Univ., Inst. Med. and Dental Eng., Professor, 医用器材研究所, 教授 (10014078)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Masato Tokyo Med. and Dental Univ., Inst. Med. and Dental Eng., Associate Professor, 医用器材研究所, 助教授 (50126231)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Vitamin D metabolites / Fluorescence-labeling / Fluorescent dienophile / Fluorometry / Chemical synthesis / Triazolinediones / Retinoic acid / Vitamin A |
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| Research Abstract |
1)To develop an accurate and precise method for assaying vitamin D metabolites in plasma, reagents to label vitamin D with fluorescence or chemoluminescence were designed and synthesized.
2)A highly fluorescent dienophile(named DMEQ-TAD), in which a fluorescent chromophore(dimethoxyquinoxalinone group)and a highly reactive dienophile(1, 2, 4-triazoline-3, 5-dione)were connected via a spacer, was successfully synthesized for the first time.
3)The reaction of the fluorescent dienophile(DMEQ-TAD)with major vitamin D_3 metabolites was examined and it was shown that all the metabolites were fluorescence-labeled in quantitative yields.
4)Fluorometry of plasma vitamin D metabolites was examined and new accurate fluorometric methods for assaying 25(OH)D_3 and 24, 25(OH)_2D_3 in plasma were developed.
5)A new and convenient stereoselective method for synthesizing 1, 25(OH)_2D_3 26, 23-lactone, a major metabolite of the active vitamin D_3, was developed.
6)Reaction of vitamin A derivatives with DMEQ-TAD was examined and it was shown that they react with the reagent with high regioselectivity indicating that the reagent might be useful for assaying the active vitamin A metabolite.
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