1991 Fiscal Year Final Research Report Summary
The physiological role of methylglyoxal pathway in mammal.
| Project/Area Number |
02670995
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Okayama University |
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Principal Investigator |
OHMORI Shinji Okayama University, Professor, 薬学部, 教授 (10032872)
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| Co-Investigator(Kenkyū-buntansha) |
TSUBOI Seiji Okayama University, Res. Assoc. Faculty of Pharmaceutical Sciences, 薬学部, 助手 (50172052)
IKEDA Mikiko Okayama University, Assistant prof., 薬学部, 助教授 (20112154)
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| Project Period (FY) |
1990 – 1991
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| Keywords | D-Lactate / Methylglyoxal / Determination / Diabetic rat / Starved rat / L-Lactate / Pyruvate / Fluorimetric |
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| Research Abstract |
1. D-Lactate in biological samples was converted into a strongly fluorescent substance in a one-vial reaction. The fluorescent substance was analysed by HPLC with fluorimetric detection. By this method 500 f mol of D-lactate could be determined. (J. Chromatogr., 566, (1991)18)
2. The change in the concentrations of D-lactate, L-lactate, pyruvate and methylglyoxal(MG)in body fluids after exercise was investigated. Plasma levels of D-lactate, L-lactate and pyruvate increased after running. MG contents in red blood 'Cells decreased markedly after running. D-lactate and related substances were also determined in urine, but the level of these substances showed no relationship to exercise. Eur. J. Appl. Physiol. in press)
3. Rats were made diabetic using streptozotocin or starved for 72 h. Diabetic and starved rats resulted in significant rise of D-lactate level in plasma, liver and skeletal muscle compared to the control group. In contrast with this, in these animals, pyruvate levels in plasma, liver and skeletal muscle fell markedly.
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