1991 Fiscal Year Final Research Report Summary
Mechanism of Cataract Formation and Searching Anticataract Agents
| Project/Area Number |
02671012
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Teikyo University |
|---|
Principal Investigator |
NISHIGORI Hideo Teikyo University, Faculty of Pharmaceutical Sciences, Professor, 薬学部・RI中央研究施設, 教授 (90050517)
|
|---|
| Project Period (FY) |
1990 – 1991
|
| Keywords | Cataract / Chick embryo / Glucocorticoid / Isocitrate / Propylene glycol / Ascorbic acid / Glutathione / Pyrroloquinoline quinine |
|---|
| Research Abstract |
Hideo Nishigori
Faculty of Pharmaceutical Sciences, Teikyo University, Sagami-ko, Kanagawa, Japan 199-01.
When hydrocortisone sodium succinate(0.25mumol/egg)is administered to 15-day-old developing chick embryos, their lenses lose transparency within 48 hrs with high frequency(>95%). The steroid-induced cataract formation depends on glucocorticoid(GC)activities and is characterized by the decline of glutathione, and the elevation of glucose, calcium, Na/K ratio and lipidperoxide(TBA-reacting substance). Morphologically, numerous vacuoles appear in perinuclear regions. A phase separation between protein and water is also observed by Raman spectroscopy. These phenomena can be effectively prevented by the radical scavengers such as ascorbic acid, tiopronin, tocopherol and pyrroloquinoline quinone, isocitrate and propylene glycol. It is likely, that the formation of GC-induced cataract of developing chick embryo appears to proceed via oxidation or peroxidation steps possibly caused by multiple activities of GC in the living system. Most of phenomena in GC-induced cataract as described above are, observed in many animal cataracts including human. The Production of cataract by the present procedure is very easy, reproducible and high frequency. GC-induced cataract model of developing chick embryo will be a useful for screening anticataract agents.
|
Research Products
(12 results)
-
-
-
-
-
-
-
-
-
-
-
[Publications] Nishigori, H., Ishida, O. and Umeda, I. O.: Preventive effects of PQQ on glucocorticoid-induced cataract formation and hepatic pigmentation in developing chick embryo "Enzymes Dependent on Pyridoxal Phosphate and Other Carbonyl Compinds as Cofactors". ed. by Fukui, T., Kagamiyama, H., Soda, K. and Wada, H. Pergamon Press, (1991)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Watanabe, A., Nishigori, H., Urakami, T., Tsuchida, T. and Hobara, N.: Pharmacological Application of Pyrroloquinoline Quinone "Priuciples and Applications of Quinoprotein". ed. by Davidsn, V. Marcel Dekker,
Description
「研究成果報告書概要(欧文)」より
