1991 Fiscal Year Final Research Report Summary
Analysis of Sweat by High Performance Liquid Chromatography
| Project/Area Number |
02680055
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
家政学
|
|---|
| Research Institution | Ochanomizu University |
|---|
Principal Investigator |
OGAWA Shojiro Ochanomizu Univ., Home Econ., Prof., 家政学部, 教授 (20013196)
|
|---|
| Project Period (FY) |
1990 – 1991
|
| Keywords | Sweat / High performance liquid chromatography / Amino acid |
|---|
| Research Abstract |
The purpose of this research is to analyze components of sweat by way of high performance liquid chromatography (HPLC) technique, and to investigate the influences of sweat on clothing materials.
In this investigation, main attention was given to the analysis of amino acids contained in the sweat, because amino acids have been found to affect the photodegradation of dye. In the first place analytical conditions to estimate amino acids contents were determined by using mixed amino acids standard solution. The pre-columm technique was applied to amino acid component labeled with 0-Phthalaldehyde and 2-mercaptoethanol. Advisable conditions are as follows : wave length : 350 nm, column : silica-ODS, eluent : aetonitrile/methanol/sodium acetate buffer solution. Under these conditions, 12 amino acids in the sweat were able to be detected. It was found that serine was contained in the largest amount in the sweat. When wool, silk, nylon and acryl fibers were immersed in the sweat, ratio of amino acid composition changed and the quantity of ammonia increased, on the other hand, cotton and polyester fibers little affected the composition of the sweat.
|
