1991 Fiscal Year Final Research Report Summary
Functional Modification of Calcium Dependent Proteins by Phosphorylation
| Project/Area Number |
02680156
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
代謝生物化学
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| Research Institution | Nagoya University |
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Principal Investigator |
KOBAYASHI Ryoji Nagoya Univ., School of Med., associate professor, 医学部, 助教授 (00020917)
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| Co-Investigator(Kenkyū-buntansha) |
HAGIWARA Masatoshi Nagoya Univ., School of Med., assistant professor, 医学部, 助手 (10208423)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Calcium Signaling / Annexin / EF-hand protein / Calcium binding protein / Calcium signal network |
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| Research Abstract |
It is generally accepted that intracellular calcium is involved in regulation various biochemical events in excitable cells. These events mediated by a family of homologous calcium-binding proteins. In this research, we focuse of EF-hand type calcium binding proteins and calcium dependent phospholipid binding proteins (Annexin family) in the many families of calcium binding proteins and tried to identify the new members of these family proteins and to reveal the function of these proteins. Firstly, we identified three proteins from smooth muscle, lens and spleen as family proteins of Annexin. Physicochemical properties of three proteins were accordance with those of Annexin family proteins such as calcium dependent phospholipid binding inhibitory action of phospholipase A_2 and actin binding. We revealed the tissue distribution using polyclonal antibodies. In the second course of this research, we purified four EF-hand type calcium binding proteins from lung, smooth muscle, aortic tissue and placenta. All these four proteins had two Ef-hand structures in those molecules and belonged to S100 protein family. In the four calcium binding proteins, calcyclin purified from rabbit lung had 50 kDa target protein in the same tissue. We identified that this protein, named CAP-50 (calcyclin associate protein with 50 kDa of molecular mass), was a family protein of Annexin. This result suggests that in intracellular calcium signalling system, different calcium binding protein had different pathways of signal and those pathways had interaction each other at the same time.
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