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1991 Fiscal Year Final Research Report Summary

The breeding of Allium species by using their cytochimeras

Research Project

Project/Area Number 02806007
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 園芸・造園学
Research InstitutionUniversity of The Ryukyus

Principal Investigator

ADANIYA Shinichi  Univ. of the Ryukyus. Coll. of Agriculture. Assistant Professor, 農学部, 助手 (20128466)

Co-Investigator(Kenkyū-buntansha) NOSE Akihiro  Univ. of the Ryukyus. Coll. of Agriculture. Associate Professor, 農学部, 助教授 (80045137)
Project Period (FY) 1990 – 1991
KeywordsBreeding / Allium / Cytochimeras
Research Abstract

The purpose of this studies is to define a possibility of improving the phenotype of Allium wakegi Araki by using its cytochimeras. The cytochimeras (4-2-2; 2-4-4) and the polyploids (2-2-2; 4-4-4) of A.wakegi and/or those of A. fistulosum L. were used for the studies, and the following subjects were investigated: 1) a method for analyzing chimerisum by the number of nucleoli per cell; 2) cytochimeral stability during propagation; 3) stomata and 4) growth characteristics; and 5) photosynthetic capability.
1. In the polyploids and cytochimeras of A. fistulosum, the ploidies of L1 and L2 could be easily identified by the maximum number of nucleoli per epidermal and mesophyll cell, respectively. While, in those plants of A. wakegi, ploidies in the layers could be also determined by the maximum number or a modal number of nucleoli per epidermal and mesophyll cell.
2. The first generation of colchicine treated plants frequently varied with their chimeral nature during in vivo propagation, whe … More reas the cytochimeras cultivated in vivo for three years after the treatment were stable for their chimerisum. On the medium containing 0.1 - 1.0 ppm BA and 0.5 ppm NAA, the polyploids and the cytochimeras drastically changed their chimerisum during in vitro propagation, but did not on the medium containing a range of 0 - 0.125 ppm BA.
3. The 4-2-2 and 4-4-4 plants had lower stomatal density and larger sized stomata than the 2-2-2 and the 2-4-4. Total stomatal pore area/mm2, which was obtained by multiplying stomatal density by stomatal size, was larger in the 4-4-4 and the 4-2-2 than in the 2-2-2 and the 2-4-4.
4. The 2-4-4 showed the highest yield under warm autumn conditions, while the 4-2-2 achieved the highest productivity under cool winter conditions.
5. Under the cool winter conditions, a stomatal conductance, a transpiration rate and a photosynthetic rate were higher in the 4-4-4 and the 4-2-2 than in the 2-2-2 and the 2-4-4.
From the above results, it is suggested that the highest productivity of the 2-4-4 under the warm conditions is attributed to its higher draft tolerance, and that the highest yield of the 4-2-2 under the cool conditions is due to its higher photosynthetic capacity under the cool conditions. The breeding of A. wakegi is, therefore, most likely activated by the use of the cytochimeras. Less

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] S.Adaniya.: "Colchine-induced Cytochimeras of Allium wakegi Araki and their growth characteristics." J.Japan Soc.Hort.Sci. 60. 105-112 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Adaniya.: "A method for determining L1 and L2 ploidy by the number of nucleoli per cell in Allium wakegi Araki." Euphytica.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Adaniya.: "Propagation of cytochimeral Allium wakegi Araki and their chimeral stability." Euphytica.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Adaniya.: "Stomatal characters in cytochimeras of Allium wakegi Araki" Euphytica.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Adaniya.: "Photosynthesis in cytochimeras of Allium wakegi Araki." J.Japan Soc.Hort.Sci.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Adaniya.: "Colchicine-induced Cytochimeras of Allium wakegi Araki and their growth characteristics." J. Japan Soc. Hort. Sci.60. 105-112 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Adaniya.: "A method for determining L1 and L2 ploidy by the number of nucleoli per cell in Allium wakegi Araki." Euphytica.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Adaniya.: "Propagation of cytochimeral Allium wakegi Araki and their chimeral stability." Euphytica.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Adaniya.: "Stomatal characters in cytochimeras of Allium wakegi Araki." Euphytica.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Adaniya.: "Photosynthesis in cytochimeras of Alloium wakegi Araki." J. Japan Soc. Hort. Sci.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-18  

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