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1991 Fiscal Year Final Research Report Summary

Isolation and molecular characterization of bovine T-cell receptor genes

Research Project

Project/Area Number 02806055
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

ISHIGURO Naotaka  Obihiro University of Agriculture and Veterinary Medicine, Department of Veterinary Public Health, Associate professor, 畜産学部, 助教授 (00109521)

Co-Investigator(Kenkyū-buntansha) HORIUCHI Motohiro  Obihiro University of Agriculture and Veterinary Medicine, Department of Veterin, 畜産学部, 助手 (30219216)
Project Period (FY) 1990 – 1991
KeywordsBovine / Lymphocyte / T cell / Receptor / Gene rearrangement / cDNA / Nucleotide sequence
Research Abstract

The nucleotide sequences of bovine T-cell receptor (TCR ; alpha, beta, gamma, and delta chains) genes which from cDNA libraries constructed from bovine peripheral blood lymphocytes and thymic lymphocytes were determined.
1. Of 30 positive cDNA clones for bovine TCR alpha chain, 20 contained complete and correct rearrangements, whereas 4 contained only one Constant (C) region, and 6 were nonfunctional because of incomplete rearrangement. The bovine Calpha gene is composed of 140 amino acid residues with striking similarity to the Ca region of human and mouse.
2. Of 38 CDNA clones for TCR beta chain, 22 were rearranged and contained the functional variable (V) gene segments. These clones were tentatively divided into nine TCR-V gene families which correspond to the human TCR-V family. Two different Cbeta gene segments were found, and both C regions were composed of 178 amino acid residues.
3. Of 9 cDNA clones for bovine TCR gamma chain isolated, only 4 were-rearranged and contained specific … More V, Joining (J), and C gene segments, but the remaining 5 contained J and C or only C gene segments without the V gene segment. Three kinds of Cgamma were found and designated as Cgamma1, Cgamma2 and Cgamma3. Compared with human and mouse Cgamma, bovine Cgamma sequences are much longer, with 27-55 amino acids corresponding to the hinge and connector regions.
4. Of three TCR delta chain CDNA clones isolated, two TCR V and three J gene segments were found. The nucleotide and deduced amino acid sequences (155 amino residues) of bovine C delta gene segment are well conserved among species.
5. During the screening for bovine TCR alpha chain gene in a bovine lymphocyte CDNA library using mouse Calpha-encoding DNA as a probe, we isolated 11 CDNA clones encoding lactate dehydrogenase (LDH) -A isozyme. These LDH-A cDNA clones coded for 332 amino acid residues which protein was identified by cellogel electrophoresis as LDH-A isozyme.
6. Sporadic bovine leukosis tumor cell lines were established by transplantation to BALB/C nu/nu mice from three types(calf, skin and thymic)of bovine lymphosarcoma, and examined for the expression of TCR alpha, beta, gamma and delta genes. Mature or truncated TCR-beta, -gamma and -delta transcripts were detected in the tumor cells, but all of them were found to be nonfunctional in cDNA cloning analysis. In the cDNA library constructed from the aberrant transcripts of the TCR-gamma gene of the thymic type of lymphosarcoma, a novel C region of the TCR gamma chain (Cgamma4) was isolated. Less

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Naotaka Ishiguro: "sequence analysis of bovine Tーcell receptor α chin" Immunogenetics. 31. 57-60 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Atsuko Tanaka: "Sequence and diversity of bovine Tーcell receptor βーchain genes" Immunogenetics. 32. 263-271 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naotaka Ishiguro: "Primary structure of bovine lactate dehydrogenaseーA isozyme and its synthesis in Escherichia coli" Gene. 91. 281-285 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuyasu Takeuchi: "Molecular cloning and sequence analysis of bovine Tーcell receptor γ and δ chain genes" Immunogenetics. 35. 89-96 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石黒 直隆: "牛T細胞抗原レセプタ-遺伝子の構造解析と牛T細胞系白血病への診断的応用" 家畜生化学研究会報. 24. 48-61 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naotaka Ishiguro: "Sequence analysis of bovine T-cell receptor alpha chain." Immunogenetics. 31. 57-60 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atsuko Tanaka: "Sequence and diversity of bovine T-cell receptor beta-chain genes." Immunogenetics. 32. 263-271 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naotaka Ishiguro: "Primary structure of bovine lactate dehydrogenease-A isozyme and its synthesis in Escherichia coli." Gene. 91. 281-285 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyasu Takeuchi: "Molecular cloning and sequence analysis of bovine T-cell receptor gamma and delta chain genes." Immunogenetics. 35. 89-96 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naotaka Ishiguro: "Sequence Analysis of bovine T-cell receptor genes and diagnostic application for bovine T-cell Iymphosarcoma." Proc. Jpn. Soc. Anim. Biochem.24. 48-61 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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