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1992 Fiscal Year Final Research Report Summary

Localization and turnover of calcium in biominerals (otoconia, bone and teeth).

Research Project

Project/Area Number 02807002
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionToyama Medical and Pharmaceutical University

Principal Investigator

KAWAMATA Seiichi  Toyama Medical and Pharmaceuitcal University Department of Anatomy Associate professor,, 医学部, 助教授 (30127641)

Project Period (FY) 1990 – 1992
KeywordsCalcium / Biominerals / Bone / Ammonium chloride / Antimony / Otoconia / Decalcification / Calcification
Research Abstract

The localization of pyroantimonate-precipitable calcium was examined in calcifying systems. Specimens were fixed by the pyroantimonate method and selectively decalcified by treatment with ammonium chloride. After this treatment, otolithic organs and young bones were easy to cut into sections and following results were obtained:
1.The fixation with pyroantimonate and glutaraldehyde (two step method) resulted in better preservation of tissue and more precise localization of precipitaes than did the direct immersion of specimens in pyroantimonate and osmium tetroxide (one step method).
2. In the endolymphatic sac of the tree frog, the precipitate was distributed around calcium carbonate crystals. When crystalline growth was accelerated, the precipitate increased in amount. These crystals were considered to grow by accretion.
3. In the otolithic organs in the inner ear of the rat and guinea pig, the precipitate was found around otoconia where a silver methenamine-positive material existed.
4. In bone of very young rats, the precipitate was frequently observed within the endoplasmic reticulum of obsteoblasts, and on vacuoles and the ruffled border of osteoclasts. The distribution of calcium pyroantimonate in decalcified bone was the same as that in undecalcified counterparts.
5. The precipitates of calcium pyroantimonate were well retained and the fine structure of the tissue was well preserved after the treatment with ammonium chloride.
6. The presence of calcium in the precipitates was confirmed by X-ray microanalysis and extraction with EGTA.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Seiichi KAWAMATA: "Fine structural changes in the endolymphatic sac induced by calcium loading in the tree frog,Hyla arborea japonica." Archives of Histology and Cytology. 53. 397-404 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiichi KAWAMATA: "Localization of pyroantimonate-precipitable calcium in the endolyphatic sac of the tree frog,Hyla arborea japonica." Archives of Histology and Cytology. 53. 405-411 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiichi KAWAMATA: "Localization of pyroantimonate-precipitable calcim in the vestibular organs of the rat and guinea pig." Archives of Histology and Cytology. 54. 173-180 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiichi KAWAMATA: "The distribution of calcium in undecalcified bone as revealed by an improved pyroantimonate method." Histochemical Journal. 24. 275-282 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiichi KAWAMATA,Yoshikazu IGARASHI: "The fine structure of the developing otolithic organs of the rat." Acta Otolaryngologica(Stockholm) Supplement. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiichi KAWAMATA,Yoshikazu IGARASHI: "Glycoconjugates in the cochlea as revealed by the silver methenamine method." Hearing Research. 67. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshikazu IGARASHI,Seiichi KAWAMATA,Kanemasa MISUKOSHI: "Glycoconjugates in the vestibular organs as revealed by the silver mehtenamine method." Hearing Research. 67. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Kawamata: "Fine structural changes in the endolymphatic sac induced by calcium loading in the tree frog, Hyla arborea japonica." Archives of Histology and Cytology. 53. 397-404 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kawamata: "Localization of pyroantimonate-precipitable calcium in the endolymphatic sac of the tree frog, Hyla arborea japonica." Archives of Histology and Cytology. 53. 405-411 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kawamata: "Localization of pyroantimonate-precipitable calcium in the vestibular organs of the rat and guinea pig." Archives of Histology and Cytology. 54. 173-180 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kawamata: "The distribution of calcium in undecalcified bone as revealed by an improved pyro-antimonate method." Histochemical Journal. 24. 275-282 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kawamata, Y. Igarashi: "The fine structure of the developing otolithic organs of the rat." Acta Otolaryngologica (Stockholm) Supplement. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kawamata, Y. Igarashi: "Glycoconjugates in the cochlea as revealed by the silver methenamine method." Hearing Research. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Igarashi, S. Kawamata, K. Mizukoshi:"Glycoconjugates in the vestibular organs as revealed by the silver methenamine method." Hearing Research. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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