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1991 Fiscal Year Final Research Report Summary

Distribution and Localization of the beta-Amyloid Precursor in Subcultured Vascular Cells.

Research Project

Project/Area Number 02807035
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionTokyo Metropolitan Institute of Gerontology

Principal Investigator

YAMAMOTO Mari  Tokyo Metro. Inst. Geront., Dept. Cell Biol., Assistant, 細胞生物学部門, 助手 (90211648)

Co-Investigator(Kenkyū-buntansha) OOYAMA T.  Tokyo. Metro. Inst. Geront. ., Dept. Cell Biol., Scientist, 生体情報部門, 研究員 (90183243)
YAMAMOTO K.  Tokyo. Metro. Inst. Geront., Dept. Cell Biol., Chief Scientist, 細胞生物学部門, 主任研究員 (90073022)
Project Period (FY) 1990 – 1991
KeywordsVascular endothelial cells / beta-APP / Localization and distribution / Deposition of beta-APP on basement membrane / Cellular aging
Research Abstract

By the immunocytochemical, immunochemical, and immunoelectron microscopic method using monoclonal antibodies against predictive synthetic beta-protein with the sequence of pesidure 597-620, the distribution and localization of beta-ainyloid protein precursor(beta-APP)in cultured vascular cells was investigated. beta-APP immunoreactivity was demonstrated in the plasma membranes of the cultured vascular cells as well as in the cytoplasma of the cells. Cytoplasmic beta-APP immunoreactivity located in the proximity of the cell nucleus and looked as microbody structure with various size. beta-APP immunoreactivity was also demonstrated on the surface of the basement membrane formed in the plastic dish during the culture of the cells. These findings suggest that the abnormal production or secretion of beta-APP in the vascular wall cells could be a cause of the formation of amyloid angiopathy in the Alzheimer's disease.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] K.Yamamoto: "Immunocytochemical identification of β-protein precursor in cultured vascular cells." Artery. 17. 213-232 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamamoto: "Distribution and localization of β-amyloid precursou (β-APP) and deposition of β-APP on the basement membrane in cultured vascular cells." Zool.Sci.7. 1165 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamamoto: "Extracellular matrix produced by cultured vascular cells." J.Jpn.Athreoscler.Soc.19. 605-610 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamamoto: "Type I collagen fibril formation by human vascular endothelial cells in culture." Artery. 19. 112-122 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Yamamoto: "Immunocytochemical identification of beta-protein precursor in cultured vascular cells." Artery. 17. 213-232 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Yamamoto: "Distribution and localization of beta-amyloid precursor (beta-APP) and deposition of beta-APP on the basement membrane in culture." Zool. Sci.7. 1165 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Yamamoto: "Extracellular matrix produced by cultured vascular cells." J. Jpn. Atheroscler. Soc.605-610 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Yamamoto: "Type I collagen fibril formation by human vascular endothelial cells in culture." Artery. 19. 112-122 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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