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1991 Fiscal Year Final Research Report Summary

Variant Endothelial cells of human aorta

Research Project

Project/Area Number 02807041
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionSaga Medical School

Principal Investigator

TOKUNAGA Osamu  Saga Medical School, Pathology Associate Professor, 医学部, 助教授 (40113229)

Co-Investigator(Kenkyū-buntansha) SHIMOKAMA Tatsuro  Saga Medical School, Pathology Research associate, 医学部, 助手 (50170999)
Project Period (FY) 1990 – 1991
KeywordsVascular endothelium / Variant endothelium / Prostacyclin / Atherosclerosis / Endotheli
Research Abstract

1) Endothelial cells in primary culture were morphologically classified into two types : typical endothelium and variant endothelium. Typical endothelial cells were small, round to polygonal, and arranged uniformly. Variant endothelial cells were larger, ranging from 100 to 200 um, and sometimes more than 250 um in diameter with multinuclei. The ratio of variant endothelial cells to typical cells correlated well with the severity of atherosclerosis than with aging and it was confirmed in experimental atherosclerotic rabbits. Variant endothelial cells can be formed by denatured LDL, interleukin-4 and oxyradicals in vitro.
2) To investigate the functional alteration of human aortic endothelial cells with aging, prostacyclin synthesis was qualitatively and quantitatively examined, and also immunohistochemically examined. endothelial cells, the young group synthesized the largest amount of prostacyclin in a conventional culture condition, with synthesis progressively decreasing in the older groups. Immunohistologically, endothelial cells in young aortas revealed intensely positive for prostacyclin, but the intensity of aortic endothelial cells from older subjects was low. Smooth muscle cells in the thickened intima of the aorta were weakly immunoreactive. The decreased synthesis of prostacyclin with age may play an important role in the development and advancement of thrombosis and atherosclerosis.
3) Interaction between endothelial cells and inflammatory cells or monocytes/macrophages is currently underinvestigation.
4) Although not directly related to this project, we developed a method of enzyme-linked immunohistochemistry for detection of "endothelin" useful on formalin-fixed and paraffin embedded sections.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Osamu Tokunaga: "Atherosclerosis- and age-related multinucleated variant endothelial cells in primary culture from human aorta" Am J Pathol. 135. 967-976 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Tokunaga: "Age-related decline in prostacyclin synthesis by human aortic endothelial cells. Qualitative and quantitative analysis" Am J Pathol. 138. 941-949 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jianglin Fan: "Alteration of functional characteristics of macrophages induced by hypercholesterolemia" Virchow Archiv B. 61. 19-27 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroyuki Kato: "Experimental Cerebral atherosclerosis in the rabbit:Scanning electron microscopic study of the intimal lesion site" Pathol Res Pract. 187. 797-805 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Teruo Watanabe: "Atherosclerosis and macrophages" Acta Pathol Jpn. 39. 473-486 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Tokunaga: "Endothelin:Immunohistologic localization in aorta and biosynthesis by cultured human aortic endothelial cells" Lab Invest. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 折茂 肇編(分担執筆、徳永 藏): "血管壁細胞の機能とその制御機構(ヒト大動脈内皮細胞の培養とプロスタサイクリン産生)" 共立出版社, 181(37-47) (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 江橋 節郎編(分担執筆、徳永 藏): "血管研究法の進歩(ヒト血管内皮細胞の培養法と特性)" 学会出版センタ-, 363(13-24) (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga O.: "Atherosclerosis and age-related multinucleated variant endothelial cells in primary culture from human aorta" Am J Pathol. 135. 967-976 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe T.: "Atherosclerosis and macrophages" Acta Pathol Jpn. 39. 473-486 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, O.: "Age-related decline in prostacyclin synthesis by human aortic endothelial cells. Qualitative and quantitative analysis" Am J Pathol. 138. 941-949 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fan J.: "Alteration of functional characteristics of macrophages induced by hypercholesterolemia" Virchow Archiv. B 61. 19-27 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato H.: "Experimental cerebral atherosclerosis in the rabbit : Scanning electron microscopic study of the intima lesion site" Pathol Res Pract. 187. 797-805 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga O.: "Endothelin : Immunohistologic localization in aorta and biosynthesis by cultured human aortic endothelial cells" Lab Invest.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada T.: "Induction of fatty streak-like lesions in vitro using a culture model system simulating arterial intima" Am J Pathol.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. T. Lee, Kogo Onodera, Kenzo Tanaka: "Atherosclerosis II. Recent progress in atherosclerosis research. Multinucleated variant endothelial cell : Its Characterization and relation to atherosclerosis. P217-222" The New York Academy of Sciences 1990.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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