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1991 Fiscal Year Final Research Report Summary

Studies on analysis of ribosomal RNA gene organization, phylogeny and evolution of spirochetes

Research Project

Project/Area Number 02807046
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionUniversity of Shizuoka

Principal Investigator

YANAGIHARA Yasutake  University of Shizuoka School of Pharmaceutical Sciences, Professor, 薬学部, 教授 (30046255)

Co-Investigator(Kenkyū-buntansha) MASUZAWA Toshiyuki  University of Shizuoka School of Pharmaceutical Sciences, Research Associate, 薬学部, 助手 (10181645)
FUKUNAGA Masahito  University of Fukuyama School of Pharmaceutical Sciences, Professor, 薬学部, 教授 (20132483)
Project Period (FY) 1990 – 1991
KeywordsSpirochetes / Leptospira / Borrelia / Treponema / Ribosomal RNA gene / Cloning / Sequencing
Research Abstract

In order to study the organization of the ribosomal RNA genes of some spirochetes the rRNA genes were cloned in phage lambda EMBL3. By subcloning the re striction fragments into pUC18 plasmid DNA and analyzing the resulting clones by Sourthern blot hybridization, a restriction map of the rRNA genes was generated and the organization of the rRNA genes was determined. The results obtained from pathogenic and nonpathogenic treponemes show that the genes for three types of rRNA (rrs, rrl, rrf) are closely linked and occur twice in their genome. Fukuna-ga et al., Microbiol. Immunol., 36, 161-167, 1992.
Correlation of genomic hybridization data with physical charactedrization of recombinant plasmid DNAs of the rRNA genes in Borrelia burgdorferi, the etiological agent of Lyme disease, showed that, in contrast to the general organization of rDNA operons ineubacteria, the rrs gene is separated from the closely linked and tandemly repeated rrl/rrf genes. Direct repeat of rRNA genes in eubacterial … More genome is the first discovery and this is a remarkable feature in the organization of the eubacterial rRNA genes. Fukunaga et al., J. Gen. Microbiol., 138, in press and Fukunaga and Sohnaka, Biochem. Bioshys. Res. Commun., in press.
To elucidate the organization of the transcription units encoding the rRNAs in the Leptospira interrogans, the nucleotide sequences of the genes and their flanking regions were determined, and the 5' termini of the transcripts were mapped by primer extention experiments. The results show that each of the rRNAs is transcribed separately, and the fact is consistent with the lack of physical linkage among them. This was the only organism known in the eubacteria, at that time, in which all the rRNA genes are present and expressed separately. We also found that there is no linkage among those in the organisms belonging to the genera Leptospira and Leptonema. Furthermore, the number of rrf gene in leptospires was correlated with their parasitism. In the strains of nonparasitic leptospires, there are two genes for rrf, whereas only one gene is carried in parasitic leptospires. Fukunaga et al., J. Bacteriol., 172, 32643268, 1990., Fukunaga et al., J. Gen. Microbiol., 137, 1523-1528, 1991. Less

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Masahito Fukunaga: "Isolation and characterization of the 55 rRNA gene of Leptospira interrogans" Journal of Bacteriology. 172. 3264-3268 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga: "Linkage of ribosomal RNA genes in Leptospira" Microbiology and Immunology. 34. 565-573 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga: "Nucleotide suquence of a 16S ribosomal RNA gene for Leptospira interrogans serovar canicola strain Moulton" Nucleic Acids Research. 18. 366 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga: "Cloning of genes for a hemolytic factor of Leptospira interrogans serovar autumnalis strain Congo 21-543" Mirobiology and Immnology. 34. 565-573 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga: "Cloning,Characterization and taxonomic significans of genes for the 5S ribosomal RNA of Leptonema illini strain 3055" Journal of General Microbiology. 137. 1523-1528 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Masuzawa: "Antigenic Properties of borrelia Burgdorferi isolated from Ixodes ovatus and Ixodes persulcatus in Hokkaido,Japan" Journal of Clinical Microbiology. 29. 1568-1573 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga: "Comparison of flanking regions of the 5S rebosomal ribonucleic acid genes in Leptospira biflexa and Leptospira interrogans" Chemical and Pharmaceutical Bulletin. 40. 544-546 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga: "Tandem repeat of the 23S and 5S ribosomal RNA genes in Borrelia burgdorferi,the etiological agent of Lyme disease" Biochemical and Biophysical Research Communication. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga: "The 23S/5S rebosomal RNA genes are separate from the 16S ribosomal RNA gene in Borrelia burgdorferi,the etiological agent of Lyme disease" Journal of General Microbiology. 138. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga: "Organization of the ribosomal RNA genes in Treponema phagedenis and Treponema pallidum" Microbiology and immunology. 36. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Philip J.Bassford: "Genetic approaches to cell biology and metabolism of spirochetes" Research in Microbiology. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福長 将仁: "ライム病ボレリアの遺伝子構成に関する研究 リボソ-ムRNA遺伝子の構成" 乳酸菌研究会に関する報告書. 346-350 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福長 将仁: "ライム病ボレリアの遺伝子構成に関する研究 リボソ-ムRNA遺伝子のクロ-ニングと制限酵素地図" 乳酸菌研究会に関する報告書. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga Ed.Yuzuru Kobayashi: "Leptospirosis 〔Isolation and characterization of the 5S rRNA genes in Leptospira and Leptonema〕" University of Tokyo Press, 534£290-303| (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamotsu Morita Ed.Yuzuru Kobayashi: "Leptospirosis 〔Cloning and expression of Leptospira interrogans hemolytic factor gene in E.coli and yeast,Saccharomyces cerevisiae" University of Tokyo Press, 534£345-357| (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Fukunaga: "Isolation and characterization of the 5S rRNA gene of Leptospira interrogans." J. Bacteriol.172. 3624-3268 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Fukunaga: "Linkage of ribosomal RNA genes in Leptospira." Microbiol. Immunol.34. 565-573 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Fukunaga: "Nucleotide sequence of a ribosomal RNA gene for Leptospira interrogans serovar canicola strain Moulton." Nucleic Acids Res.18. 366 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Fukunaga: "Cloning of genes for a hemolytic factor of Leptospira interrogans serovar autumnalis strain Congo 21-543." Microbiol. Immunol.34. 565-573 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Fukunaga: "Cloning, characterization and taxonomic significans of genes for the ribosomal RNA of Leptonema illini strain 3055" J. Gen. Microbiol.137. 1523-1528 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Masuzawa: "Antigenic properties of Borrelia burgdorferi isolated from Ixodes ovatus and Ixodes persulcatus in Hokkaido, Japan." J. Clin. Microbiol.29. 1568-1573 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Fukunaga: "Comparison of flanking regions of the 5S ribosomal ribonucleic acid genes in Leptospira biflexa and leptospira interrogans." Chem. Pharm. Bull.40. 544-546 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Fukunaga: "Tandem repeat of the 23S and 5S ribosomal RNA genes in Borrelia burgdorferi, etiological agent of Lyme disease." Biochem. Biophys. Res. Commun.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Fukunaga: "The 23S/5S ribosomal RNA genes are separate from the 16S ribosomal RNA gene in Borrelia burgdorferi, the etiological agent of Lyme disease." J. Gen. Microbiol.138. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Fukunaga: "Organization of the ribosomal RNA genes in Treponema phagedenis and Treponema pallidum." Microbiol. Immunol.36. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Philip J. Bassford.: "Genetic approaches to cell biology and metabolism of spirochetes." Res. microbiol.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Fukunaga.: "Isolation and characterization of the 5S rRNA genes in Leptospira and Leptonema." Leptospirosis. 290-303. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamotsu Morita: "Cloning and expression of Leptospira interrogans hemolytic factor gene in E. coli and yeast, Saccharomyces cerevisiae." Leptospirosis. 345-357. (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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