1992 Fiscal Year Final Research Report Summary
Molecular Biological Approach to Nephritogenic Proteins
| Project/Area Number |
02807071
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
MORITA Tsubasa Univ.Occu. Env. Health ; Dept.Internal Medicine Associate Prof., 医学部, 助教授 (70131943)
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| Co-Investigator(Kenkyū-buntansha) |
GOTOH Sadao Univ.Occu. Env. Health ; Dept Biochemistry Associate Prof., 医学部, 助教授 (50131917)
HIGASHI Ken Univ.Occu. Env. Health ; Dept. Biochemistry Professor, 医学部, 教授 (30028386)
HIRANO Hideyasu Univ.Occu. Env. Health ; Dept. Biochemistry Assistant Prof., 医学部, 講師 (50040241)
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| Project Period (FY) |
1990 – 1992
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| Keywords | Liver / Profilin / Fibronectin / ECM / Basement Membrane / Collagen / Morphological Control / cirrhosis / Northern |
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| Research Abstract |
If a protein is controlling morphology, it will appear during morphology change. We investigated according to this hypothesis to obtain or to discover controlling morphology proteins. We used antibodies against basement membranes to obtain clones carrying cell surface vicinal proteins. Several inserts cDNAs obtained from lambdagtll cDNA libraries were used as probes for Northern blot hybridization. The 21st probe fragment(NH21) showed an increased level of expression in early liver fibrosis induced by a single shot of carbon tetrachloride(CCl_4). Sequenced NH21 was homologous to human profilin cDNA. Fibronectin mRNA began increasing after a shot of CCL_4 and peaked after 6 hrs to approximately the 4 fold level. The increase of fibronectin mRNA preceded and predominated over the increases of procollagen mRNAs which were measured at the same time after a single shot. The collagen alpha2(I), alpha1(III), alpha1(IV), Laminin B1, and B2 also increased. The transcription peaks of them was 6 hours after CCl_4 injection, except collagen alpha1(III) which was 1.5 hours. These are extracellular matrix proteins and acting as morphology fixers. We propose that there are 3 types of controlling morphology proteins. Profilin, fibronectin, and gelsolin are the first type of these proteins. Collagens and laminin are the second type of fixing morphology proteins. CRE and TRE binding proteins are the third type of transacting inducer proteins of those proteins.
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[Publications] Keiroh Utsumi, Motoyuki Sawada, Seiji Narumiya, Jun Nagamine, Tsuneaki Sakata, Shoji Iwagami, Yasumichi Kita, Hiroshi Teraoka, Hideyasu Hirano, Masato Ogata Toshiyuki Hamaoka, and Hiromi Fujiwara: "Adhesion of immature thymocytes to thymic stromal cells through fibronectin molecules and its significance for the induction of Thymocyte differentiation" Proc Natl Acad Sci USA. 88. 5685-5689 (1991)
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