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1991 Fiscal Year Final Research Report Summary

Detection of hepatitis C virus RNA by sensitive PCR method

Research Project

Project/Area Number 02807072
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionChiba University

Principal Investigator

YOKOSUKA Osamu  Chiba University, assistant, 医学部, 助手 (90182691)

Co-Investigator(Kenkyū-buntansha) IMAZEKI Fumio  Chiba University, assistant, 医学部, 助手 (40223325)
Project Period (FY) 1990 – 1991
KeywordsPolymerase chain reaction / Hepatitis C virus / HCV RNA / Synthetic DNA / Primer / Acute hepatitis / Chronic hepatitis / Interferon
Research Abstract

Non-A, non-B hepatitis virus is a major etiologic agent of chronic liver disease in Japan. Recently, M. Houghton et al. mleculary cloned the hepatitis C virus(HCV)nucleic acid and reported its genomic sequence. An assay system for detection of anti-HCV antibody was also established. However, detection method for HCV antigen or HCV nucleic acid have not been established. The reason might probably be because of small amount of the virus in spechmns. Here we tried to establish a sensitive method for detection of HCV RNA by applying polymerase chain reaction(PCR)which is a powerful method to detect small amount of nucleic acid. To detect the HCV RNA, we extracted the nucleic acid from patients samples(blood and liver tissue), then reversetranscribed the nucleic acid to cDNA, then amplified with PCR method using synthetic oligonucleotide primers. The primes used were synthesized based on the HCV sequence and flaviviral sequences. With this RT-PCR rmthod we detected the HCV RNA in the sera from patients with non-A, non-B hepatitis. HCV RNA was detectable in 83 of 90(92%)patents. It was detectable in liver tissue in 36 of 40(90%)patients. Thus, it was revealed that mst of patients with non-A, non-B hepatitis were infected with hepatitis C virus. We also detected the change of HCV RNA in patients treated by interferon. When patients were effectively treated, HCV RNA in serum persistently disappeared, in contrast it remained positive when the treatment was resuccessful. Detection of HCV RNA seems to indicate whether the treatment is effective or not. The developrant of detection method of HCV RNA seems to be important for the diagnosis and treatment of type C hepatitis.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Yokosuka Osamu: "Detection and direct sequeneing of hepatitis B virus genomec by DNA amplification method." Gastro enterology. 100. 175-181 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato Naoya: "Defection of hepatitis C virus rebonucleic acid in the serum by amplilication with pdymerase chan reaction." J.Clinical Investigation. 86. 1764-1767 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosoda Kazuhiko: "Detection and partial sequencing of hepatitis C virus RNA in the liver" Gastro enterology. 101. 766-771 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横須賀 收: "インタ-フェロンαのC型肝炎ウイルスに対する抗ウィルス効果" 肝臓. 32. 308-309 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤 直也: "Competitive RTーPCR(CRTーPCR)法によるC型肝炎ウィルス量の測定とインタ-フェロン治療効果" 肝臓. 32. 750-751 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤 直也: "PCR法を用いたC型肝炎ウィルスRNAの検索" 肝臓. 31. 705-706 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokosuka O, et al.: "Detection and direct sequencing of hepatitis B virus genome by DNA amplification method." Gastroenterology 100. 175-181 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato N, et al.: "Detection of hepatitis C virus ribonucleic acid in the serum by amplification with polymerase chain reaction." J clin Invest. 86. 1764-1767 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosoda K, et al.: "Detection and partial sequencing of hepatitis C virus RNA in the liver." Gastroenterology. 101. 766-771 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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