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1991 Fiscal Year Final Research Report Summary

Trial of neuronal regeneration after spinal cord injury by implantation of cultured astrocytes and by neurotrophic factor

Research Project

Project/Area Number 02807132
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionOsaka University

Principal Investigator

YAMADA Kazuo  Osaka University Neurosurgery Assistant Professor, 医学部, 講師 (90150341)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Toru  Osaka University Neurosurgery Professor, 医学部, 教授 (20135700)
Project Period (FY) 1990 – 1991
KeywordsSpinal Cord Injury / Head Injury / Ischemic Injury / Growth Factor / Glia / BFGF / Neuronal Regeneration
Research Abstract

We developed a model of spinal cord injury by transection of the posterior column of the spinal cord. The animals survived after injury and the area of injury was constant. Therefore the model is suitable for studying neuronal recovery from spinal cord injury. We cultured fetal astrocytes and marked them by PHA-L. The astrocytes were first cultured on the membrane filter, and the filter with confluent cells was applied to the injured cavity. The transplanted astrocytes survived in the injured site of the spinal cord, and have some modification the injured axons. To study basic data for injury of the central neurons, we then studied the brain injury model. We studied expression of the basic fibroblast growth factor(bFGF)and its receptor. The method we used are in situ hybridization for expression of messenger RNA, and immunostaining and Western blotting for protein expression. The bFGF receptor mRNA was expressed in the neurons and astrocytes of the periinfarcted area one day after injury. Expression of the FGF mRNA however, delayed till 3 days after injury. On the other hand, expression of FGF receptor messenger RNA occurred one day after surgery. The results indicate that neurons express their bFGF receptor firstly, and if there is no supply of FGF from surrounding environments, they make de novo synthesis of FGF.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Kohmura E: "Recombinant human basic fibroblast growth factor(CS23)prevents retrograde degeneration of thalamic neurons after ablation of the sensory cortex" Advances in Neurotrauma Research. 2. 58-63 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kinoshita A: "Effects of astrocyte-derived factors on ischemic brain edema induced by rat MCA occlusion" APMIS. 98. 851-857 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada K: "Basic fibroblast growth factor prevents thalamic degeneration after cortical infarction" J Cereb Blood Flow Metabol. 11. 472-478 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kinoshita A: "Wound healing following stab injury on rat cerebral cortex" Neurol Res. 13. 184-188 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 和雄: "梗塞周囲脳における塩基性線維芽細胞成長因子(bFGF)の発現と外因性bFGFによる梗塞後視床変性の修飾" 神経化学. 30. 90-91 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakaguchi T: "Expression of bFGF receptor mRNA in the central nervous system with ischemic injury" Advances in Neurotrauma Research. 3. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada K: "Modification of ischemic neuronal injury by basic fibroblast growth factor.Ito U,Klatzo I(eds)Maturation Phenomenon" Springer, (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada K: "Expression of basif FGF in periinfarcted brain tissue and modification of postischemic thalamic degeneration by exogenous basic FGF.In Globus MY-T,Dietrich WD(eds):The Role of Neurotransmitters in Brain Injury" Plenum publishing, (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohmura E, et al: "Recombinant human basic fibroblast growth factor (CS23) prevents retrograde degeneration of thalamic neurons after ablation of the sensory cortex" Advances in Neurotrauma Research. 2. 58-63 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinoshita A, et al: "Effects of astrocyte-derived factors on ischemic brain edema induced by rat MCA occlusion" APMIS. 98. 851-8557 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada K, et al: "Basic fibroblast growth factor prevents thalamic degeneration after cortical infarction" J Cereb Blood Flow Metabol. 11. 472-478 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinoshita A, et al: "Wound healing following stab injury on rat cerebral cortex" Neurol Res. 13. 184-188 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakaguchi T, et al: "Expression of bFGF receptor mRNA in the central nervous system with ischemic injury" Advances in Neurotrauma Res. 3. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada K, et al: "Modification of ischemic neuronal injury by basic fibroblast growth factor." Maturation Phenomenon. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada K, et al: "Expression of basic FGF in periinfarcted brain tissue and modification of postischemic thalamic degeneration by exogenous basic FGF" The Role of Neurotransmitter in Brain Injury. (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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