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1992 Fiscal Year Final Research Report Summary

USE OF RECONSTITUTED BASEMENT MEMBRANE EXTRACTS TO STUDY OF THE MECHANISMS OF THE INVASION OF MALIGNANT TUMORS THROUGH BASEMENT MEMBRANES

Research Project

Project/Area Number 02807140
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

IWAMOTO Yukihide  KYUSHU UNIVERSITY,FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (00213322)

Project Period (FY) 1990 – 1992
Keywordsmalignant tumors / metastasis / basement membrane / invasion
Research Abstract

Basement membrane are thin extracellular structures surrounding most epithelial tissues, nerves and muscles as well as the lining of most blood and lymph vessels. Tumor invasion of basement membrane is a crucial step in the multistage process which leads to the formation of metastasis. Tumor cells cross basement membranes as they initially invade the lymphatic or vascular beds during dissemination, and they penetrate into their target tissue. It is likely that the invasion of tumor cells through basement membranes involves distinct events, including: a) attachment of tumor cells to laminin in the basement membranes through cell surface receptors; b) secretion of enzymes by tumor cells that cause the degradation of basement membrane barrier, and c) migration of tumor cells into the target tissue. we have used the reconstituted basement membrane molecules formed into barriers in order to investigate the invasiveness of malignant bone and soft tissue tumor cells in vitro. Results obtained from our study were as follows: 1) HT1080 human fibrosarcoma cells attach to the basement membrane via four attachment sites of laminin (YIGSR,PDSGR,RGD,IKVAV) and type IV collagen.
2) The binding of tumor cells to laminin in the basement membranes were found to induce the secretion of collagenase IV and motility both of which are crucial factors for the invasion.
3) Cyclic AMP analogues and a synthetic peptide, Tyr-Ile-Gly-Ser-Arg (YIGSR), from the B1 chain of laminin were able to suppress the invasiveness of HT1080 human fibrosarcoma cells. These agents were also found to reduce the lung colonization in vivo.
4) Not only the cell lines but also the primary cultures of malignant bone and soft tissue tumors showed invasiveness in vitro while non-metastatic cells failed to do so.
5) Co-injection of basement membrane extracts facilitated the transplantability of human malignant bone and soft tissue tumors obtained at surgery into nude mice.

  • Research Products

    (82 results)

All Other

All Publications (82 results)

  • [Publications] Yukihide Iwamoto: "Comparsion of the effect of intra-arterial and intravenous cisplatin on both limb tumor and lung metastases of transional cell carcinoma RBT-1 in rats." Chemotherapy. 36. 294-299 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "骨軟部腫瘍のMRI" 医学のあゆみ. 152. 696-699 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 奥山 清隆: "cyclic AMPによるヒト線維肉腫細胞の増殖および浸潤能の抑制" 整形外科と災害外科. 38. 1579-1582 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田仲 和宏: "骨軟部腫瘍のMRI-軟骨系腫瘍について-" 整形外科と災害外科. 39. 638-642 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 牛島 正博: "大腿骨近位部悪性骨腫瘍に対する大腿骨近位端置換術例の検討" Hip Joint. 16. 371-373 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉岡 洋一: "人工関節を用いない再建術-同種骨移植-" 医学のあゆみ. 156. 97-100 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Use of a reconstituted bosement membrance extract to fascilitate the transplantability of human tumors in nude mice" Med.Sci.Res,. 19. 223-224 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Efficacy of two-route chemotherapy using cisplantin and its antidote,sodium thiosulfate regionally confined malignancy." Acta Orthop.Scand.62. 70-71 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "骨軟部腫瘍のMRI-質的診断の可能性-" 整形外科と災害外科. 39. 1714-1717 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大賀 正義: "腰椎変性辷り症に対する腰椎前方固定術の検討" 西日本脊椎研究会誌. 17. 77-81 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中馬 広一: "腓骨の遠位端に発生した悪性腫瘍の2例について" 整形外科と災害外科. 40. 498-502 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 細川 哲: "軟部悪性腫瘍に対する追加広範切除術について" 整形外科と災害外科. 40. 527-529 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大賀 正義: "CDIによる脊柱側彎症の治療経験-derotation効果について-" 整形外科と災害外科. 40. 158-160 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 牛島 正博: "骨肉腫に対する術前局所道注療法の臨床成績と局所病巣と肺転移巣の組織学的検討" 整形外科と災害外科. 39. 1733-1736 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大賀 正義: "CDIにおいて治療せる特発性脊柱側彎性の矢状面カーブの検討" 整形外科と災害外科. 39. 1160-1163 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "A new technigue for the reduction and stabitization of spondylolisthesis" Orthopaedics International Edition. 1. 86-91 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Use of a reconstituted basement membrane to study the invasiveness of tumor cells" Adv.Exp.Med.Biol.324. 141-149 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Surgical treatment of metastafic tumor of the long bone and the spine" Adv.Exp.Med.Biol.324. 295-303 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "YIGSR,a synthefic laminin peptide,inibits the enbacement by cyclophosphamide of experimental lang metastasis of human fibrosacoma cells" Clin.Expl.Metastasis. 10. 183-189 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Wide resection and massive segmental allograft replacement in the treatment of malignant on agressive tumors" Muskuloskeletal Tissue Banking and Grafting. 183-185 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Massive segmental allograft replacement in the treatment of bone tumors:long-ternm follow-up" Low Temperature Medicine. 17. 148-150 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Comparison of the effects of intra-arterial and intravenous Clsplatin on both limb tumor and lung metastases in rats" Orthop.Transactions. 441. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Cyclic AMP decreases invasiveness and the metastafic potential of H-ras transformed mouse fibroblast" Orthop.Transactions. 440. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "An in vitro invasion assay using a reconstituted basement membranto study the invasiveness of tumor cells" Orthop.Transactions. 521-522 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Co-injection with basement membrane extracts facilitates the transplantability of human tumors obtained at surgery in nudemice" Med.Sci.Res.20. 663-664 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本 卓明: "悪性腫瘍のMRI-化学療法効果について-" 整形外科と災害外科. 40. 1667-1671 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 浦田 正彦: "間葉性軟骨肉腫2例について" 整形外科と災害外科. 40. 1714-1717 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩熊 智雄: "化学療法剤による腎機能、電解質異常の経験について" 整形外科と災害外科. 41. 512-515 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中馬 広一: "骨肉腫に対する術前化学療法の効果判定の問題点" 整形外科と災害外科. 40. 1677-1679 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "がん骨転移の基礎" 整形外科Mook. 63. 1-10 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Two-route chemotherapy using and its antidote,sodium thiosulfate for regionally confined malig nancy" OHhop.Transations,in press,. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "Co-injection with basement membrance extracts facilitate the transplantabiloty of malignant bone and soft tissue tumors in nude mice" Orthop.Transaction,in press. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukihide Iwamoto: "A synthetic laminin peptide inhibits the enhacement by cyclophosphamide of experimental metastasis of fibrosarcoma cells" Orthop.Transaction,in press. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "ヒト線維肉腫細胞の基底膜内浸潤の機序について(第2報)" 日整会誌. 64. S991 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "骨肉腫に対するCisplatin動注療法:実験結果と臨床成績の対比" 日整会誌. 64. S701 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大賀 正義: "頸椎後縦靱帯骨化症に対する頸部脊柱管拡大術の手術成績" 日整会誌. 65. S268 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "悪性骨腫瘍に対する同種骨移植を用いた患肢温存手術の検討" 日整会誌. 65. S338 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中馬 広一: "骨肉腫の術前化学療法の効果判定について" 日整会誌. 65. S880 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三好 賢一: "Cisplatinの成長軟骨の及ぼす影響について" 日整会誌. 65. S1364 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "ヒト線維肉腫細胞の基底膜内浸潤の機序について" 日整会誌. 65. S1357 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小田 義直: "動脈瘤様骨嚢腫類似の変化を判った骨巨細胞腫の臨床病理学的研究" 日整会誌. 65. S845 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "骨巨細胞腫長期観察例の再発と術後機能について" 日整会誌. 65. S755 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "腫瘍生物学的見地から見た骨肉腫の化学療法" 日整会誌. 66. S7 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "MRIによる軟部腫瘍の質的診断の可能性:病理組織像との対比" 日整会誌. 66. S838 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中馬 広一: "肩関節周囲の悪性骨軟部腫瘍に対する後方からの手術方法" 日整会誌. 66. S849 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田仲 和宏: "ラット後肢腫瘍、肺転移及び延命率に対するシスプラチン動注及び全身投与の比較" 日整会誌. 66. S1546 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田仲 和宏: "悪性神経上皮腫瘍細胞株におけるCAMP及び神経成長因子による神経分化の誘導" 日整会誌. 66. S1395 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "Basement Membrane Extractによるヒト悪性骨軟部腫瘍のヌードマウス可移植率の促進" 日整会誌. 66. S1378 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本 幸英: "制癌剤Cyclophosphamideによるヒト線維肉腫細胞の実験的肺転移促進とYIGSRによる防止効果" 日整会誌. 66. S1373 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwamoto,Y. et al.: "Comparison of the effect of intra-arterial and intra-venous cisplatin on both limb tumor and lung metastasis of transitional cell carcinoma RBT-1 in rats." Chemotherapy. 36. 294-299 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Magnetic resonance imaging of bone and soft tissue tumors" Igaku-no-Ayumi in Japanese. 152. 696-699 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuyama,K.,Iwamoto,Y. et al.: "Reduction of growth and invasive activity of human HT1080 fibrosarcoma cells by cAMP" Orthopaedics&Traumatology in Japanese. 38. 1579-1582 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka,K.,Iwamoto,Y. et al.: "Magnetic resonance imaging of bone and soft tissue tumors:MRI of cartilagenous tumors" Orthopaedics&Traumatology in Japanese. 39. 638-642 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ushijima,M.,Iwamoto,Y.et al.: "Treatment of the malignant bone tumor of the proximal femur by endoprosthetic replacement" Hip Joint in Japanese. 16. 371-373 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugioka,Y. and Iwamoto,Y.: "Limb salvage and allograft replacement for bone tumors of the extremities" Igaku-no-Ayumi in Japanese. 156. 97-100 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Use of reconstituted basement membrane extract to facilitate the transplantability of human tumors in nude mice" Med. Sci. Res.19. 223-224 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Efficacy of two-route chemotherapy using cisplatin and its antidote, sodium thiosulfate for regionally confined malignancy" Acta Orthp. Scand 62. Suppl 246. 70-71 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Magnetic resonance imaging of bone and soft tissue tumors:patterns of intensity inside of tumor tissues" Orthopaedics&Traumatology in Japanese. 39. 1714-1717 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oga M.,Iwamoto,Y. et al.: "Clinical study of anterior lumber interbody fusion for the treatment of degenerative spondylolisthesis" J.West.Jap.Res.Soc. Spine in Japanese. 17. 77-81 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chuman,H.,Iwamoto,Y. et al.: "Two cases of the malignant bone tumor of the distal fibula" Orthopaedics&Traumatology in Japanese. 40. 498-502 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosokawa,A.,Iwamoto,Y. et al.: "Additional wide resections for the patients with malignant soft part tumors" Orthopaedics&Traumatology in Japanese. 40. 527-529 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oga,M.,Iwamoto,Y., et al.: "Analysis of derotational effect of CDI in idiopathic scoliosis" Orthopaedics&Traumatology in Japanese. 40. 158-160 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ushijima,M.,Iwamoto,Y. et al.: "Clinico-pathological analysis of osteosarcoma treated with intra-arterial administration of CDDP as preoperative chemotherapy" Orthopaedics&Traumatology in Japanese. 39. 1733-1736 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oga,M.,Iwamoto,Y. et al.: "Sagittal plane analysis in idiopathic scoliosis patients treated with Cotrel-Dubousset instrumentation" Orthopaedics&Traumatology in Japanese. 39. 1160-1163 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "A new technique for the reduction and stabilization of spondylolisthesis" Orthopaedics International Edition. 1. 86-91 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Use of a reconstituted basement membrane to study the invasiveness of tumor cells" Adv.Exp.Med.Biol.324. 141-149 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Surgical treatment of metastatic tumor of the long bone and the spine" Adv. Exp.Med.Biol.324. 295-303 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "YIGSR, a synthetic laminin peptide, inhibits the enhancement by cyclophosphamide of experimental lung metastasis of human fibrosarcoma cells" Clin.Expl. Metastasis. 10. 183-189 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Wide resection and massive segmental allograft replacement in the treatment of malignant or agressive tumors" Musculoskeletal Tissue banking and Grafting. 183-185 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Massive segmental allograft replacement in the treatment of bone tumors: long-term follow-up" Low Temperature Medicine. 17. 148-150 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Comparison of the effect of intra-arterial and intravenous cisplatin on both limb tumor and lung metastasis in rats" Orthop. Transactions. 441 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Cyclic AMP decreases invasiveness and the metastatic potential of H-ras transformed mouse fibroblasts" Orthp. Transactions. 440 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "An in vitro invasion assay using a reconstituted asement membrane to study the invasiveness of tumor cells" Orthp. Transactions. 521-522 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Co-injection with basement membrane extracts facilitates the transplantability of human tumors in nude mice" Med. Sci. Res.20. 663-664 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto,T.,Iwamoto,Y. et al.: "Evaluation of chemotherapeutic efficacy by magnetic resonance imaging" Orthopaedics&Traumatology in Japanese. 40. 1667-1671 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Urata,M.,Iwamoto,Y. et al.: "Mesenchymal chondrosarcoma: a report of two cases" Orthopaedics&Traumatology in Japanese. 40. 1714-1717 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwakuma,T.,Iwamoto,Y. et al.: "Renal toxicity of chemotherapy for bone and soft tissue sarcomas" Orthopaedics&Traumatology in Japanese. 41. 512-515 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chuman,H.,Iwamoto,Y. et al.: "The correlation between histological responces and clinico-radiographycal responces in neoadjuvant chemotherapy for osteosarcoma" Orthopaedics&Traumatology in Japanese. 40. 1677-1679 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Biological aspects of bone metastasis" Seikeigeka MOOK. 63. 1-10 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Two-route chemotherapy using cisplatin and its antidote, sodium thiosulfate for regionally confined malignancy" Orthopaedic transactions. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "Co-injection with basement membrane extracts facilitate the transplantability of malignant bone and soft tissue tumors in nude mice" Orthopaedic Transactions. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto,Y. et al.: "A synthetic laminin peptide inhibits the enhancement by cyclophosphamide of experimental metastasis of human fibrosarcoma cells" Orthopaedic Transactions. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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