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1992 Fiscal Year Final Research Report Summary

Mechanism of essential hypertension in aspect of molecular endocrionology, especialy involvement of ANP and BNP.

Research Project

Project/Area Number 02807217
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionShowa University

Principal Investigator

UZAWA Ryuichi  Showa University, School of Medicine, Assistant Professor., 医学部, 講師 (80160232)

Co-Investigator(Kenkyū-buntansha) GOMI Kunihide  Showa University, School of Medicine, Professor, 医学部, 教授 (60053980)
TAKAGI Yasushi  Showa University, School of Medicine, Associate Professor., 医学部, 助教授 (30138490)
FUKUCHI Kunihiko  Showa University, School of Medicine, Assistant Professor., 医学部, 講師 (70181287)
Project Period (FY) 1990 – 1992
KeywordsBovine adrenal glomerulosa cells / Angiotensin II / ANP / BNP / Protein kinasc C / c-myc
Research Abstract

In this study, we investigated the role of ANP and BNP in essential hypertension and the mechanism of ANP and BNP action on if. we adopted cultured bovine adrenal cells as an experimental model. Our experiment showed that ANP and BNP suppressed aldosterone secretion from bovine adrenal cells. ANP and BNP also suppressed angiotensin II action on adrenal cells. It is said that cGMP express the physiological actions of ANP and BNP as a second messenger in the signal transduction pathway. However, on aldosterone secretion, ANP and BNP use another signal transduction pathway, not through cGMP accumulation. Our results suggested that this unknown pathway was closely related to suppression of protein kinase C.For this experiment, we contrived new method of determination of protein kinase c by using flowcytometrical method. Adoption of this method made our results objective. We conclude that the elucidation of the mechanism of ANP and BNP on the suppression of aldosterone secretion aids to understand the nature of essential hypertension from the point of view of hormonal regulation of blood pressure.
We challenged by using another manner for the same purpose as mentioned above. we studied c-myc oncogene expression under the influence of ANP and BNP.In cultured bovine adrenal cells, ANP and BNP both suppressed the expression of c-myc oncogene. c-myc oncogene plays an important role in cell proliferation. In bovine adrenal cells, the protein kinasc C promoted expression of c-myc oncogene so that ANP and BNP suppress the expression of c-myc by way to unknown pathway, closely related to suppression of protein kinase C.The relation between cell proliferation and hormone secretion still remains unknown, and the involvement of ANP and BNP on the secretory regulation and c-myc expression regulation is also unknown. we are planning now further experiment from another aspect.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 鵜澤龍一: "血清CKに関する最近の知見" 臨床病理. 38. 282-287 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鵜澤龍一,金 正基,平沢政人,細谷純一郎,高木 康,五味邦英: "血清Creatine Kinase MMサブバンド変換因子の物理化学的性状 -第二報-" 昭和医学会雑誌. 50. 477-482 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim,J,-G.,Y.Takagi,R.Uzawa,K.Gomi and K.Sugita: "Clinical Significance of CK-MM Isoform Analysis for the Diagnosis of Neuromuscular Diseases." Showa Univ.J.Med.Sci.3. 125-131 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢沢直行,高木 康,五味邦英,鵜澤龍一: "臨床検査教育双書3" 近代出版, 26 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鵜澤龍一,五味邦英: "臨床検査ガイド92" 文光堂, 2 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ryuichi Uzawa: "The Current Aspect of Serum Creating Kinase in the Laboratory Medicine" Jpn.H.Clin.Pathol.38(3). 282-287 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ryuichi Uzawa, J.G.Kim, M.Hirasawa, J.Hosoya, Y.Takagi, K.Gomi: "Physico-chemical properties of the CK-MM subband converting factor in human serum" J.Showa Med.Assoc.50(5). 477-482 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim, J.G., Y.Takagi, R.Uzawa, K.Gomi and K.Sugita: "Clinical Significance of CK-MM Isoform analysis for the Diagnosis of Neuromuscular diseases" Showa Univ.J.Med.Sci.3(2). 125-131 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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