1993 Fiscal Year Final Research Report Summary
DNA analysis on developmental anomalies and malignant tumors for the investigation on the mechanism of carcinogenesis.
| Project/Area Number |
03045045
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | University-to-University Cooperative Research |
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| Research Institution | Tokai University |
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Principal Investigator |
WATANAVE Keiichi Tokai University School of Medicine, 医学部, 教授 (00055865)
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| Co-Investigator(Kenkyū-buntansha) |
NABER Stephan p. Tufts Univ., School of Medicine, Sch. of Med. ・Dept. of Pathol., Assistant
WOLFE Hubert j. Tufts Univ., School of Medicine, Sch. of Med. ・Dept. of Pathol., Professor
TSUTSUMI Yutaka Tokai University School of Medicine, 医学部, 助教授 (80138643)
MORIUCHI Tetsuya Tokai University School of Medicine, 医学部, 助教授 (20174394)
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| Project Period (FY) |
1991 – 1993
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| Keywords | p53 protein / colonic cancer in adenoma / point mutation / in situ PCR technique / immunohistochemistry / antigen retrieval / homeo box gene / Hox4B protein |
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| Research Abstract |
1. Antigen retrieval method for p53 protein in formalin-fixed, paraffin-embedded sections were established. Nuclear localization of p53 protein in adenocarcinoma cells was reproducibly observed by treating deparaffinized sections with 90ﾟC heating for 120 min in PBS. Normal cells remained completely negative. Dysplastic cells in adenomas also expressed p53 protein, but it was mainly seen after antigen retrieval. The degree of p53 antigen retrieval was dependent upon the degree of cellular dysplasia, suggesting the possibility that the mutant p53 protein forms complexes with the wild-type protein in adenoma cells.
2. Technical aspects of in situ PCR was presented and demonstrated by Prof.Wolfe either at the seminar in Tokai University or in the lab of Tufts University. This technique is particularly useful in high-sensitivity detection of the genome of pathogens such as HIV and EBV.
3. Polyclonal and monoclonal antibodies against the Hox4B protein encoded by a homeo box gene were established. By immunohistochemical screening, Hox4B protein was expressed in normal and neoplastic human adrenal medulla. Expression of the Hox4B protein was also seen in PCNA-negative cells in rat fetus, suggesting that its expression is closely related to the programmed cell death.
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Research Products
(9 results)
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[Publications] Taniguchi,Y.,Inoko,H.,Iwasaki,K.,Fujii,A.,Suemizu,H.,Yoshimura,S and Moriuchi,T.: "Structural analysis of the human HOX4A homeobox gene." Nucleic Acids Res.25. 31-32 (1991)
Description
「研究成果報告書概要(和文)」より
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[Publications] Hamana,T.,Kawai,K.,Serizawa,A.,Tsutsumi,Y.,Watanabe,K.: "Immunohistochemical demonstration of p53 protein in colorectal adenomas and adenocarcinomas.Reliable application of the heat-induced antiger retrieval method to formalin-fixed,paraffin-embedded material." Pathol.Intern.in press. (1994)
Description
「研究成果報告書概要(和文)」より
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[Publications] Taniguchi,Y., Inoko,H., Iwasaki,K., Fujii,A., Suemizu,H., Yoshimura,S. and Moriuchi,T.: "Structural analysis of the human HOX4A homeobox gene." Nucleic Acids Res.25. 31-32 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hamana,T., Kawai,K., Serizawa,A., Tsutsumi,Y., Watanabe,K.: "Immunohistochemical demonstration of p53 protein in colorectal adenomas and adenocarcinomas. Reliable application of the heat-induced antigen retrieval method to formalin-fixed, paraffin-embedded material." Pathol.Intern.(in press). (1994)
Description
「研究成果報告書概要(欧文)」より
