1991 Fiscal Year Annual Research Report

細胞増殖,分化の制御における核内癌遺伝子の機能

Research Project

Project/Area Number	03151059
Research Institution	Tokyo University of Science
Principal Investigator	小田鈎一郎東京理科大学, 基礎工学部, 教授 (40012736)
Co-Investigator(Kenkyū-buntansha)	木村元喜九州大学, 生体防御医学研究所, 教授 (00031964) 岡山博人大阪大学, 微生物病研究所, 教授 (40111950) 井出利憲広島大学, 医学部, 教授 (60012746) 伊庭英夫東京大学, 医科学研究所, 助教授 (60111449) 山口宣生東京大学, 医科学研究所, 教授 (90012723)
Keywords	核内癌遺伝子 / 癌抑制遺伝子 / 細胞周期 / 転写制御因子 / CDNAライブラリィ / 温度感受性変異株 / 細胞のトランスフォ-メ-ション
Research Abstract	1.E1Aによりフイブロネクチン遺伝子の転写抑制因子が誘導されること、その誘導にはRBとの結合領域が必要であることを示した(小田)。野生型p53がRB遺伝子の転写を抑制すること、その抑制には2つのp53responsive elementsが必要であることを示した(山口)。Fra1,Fra2は複合体を形成するJunフアミリ-のメンバ-の種類によりAP1モチ-フをもつ遺伝子の転写を正にも負にも制御しうることを示した(伊庭)。Junフアミリ-の新しいメンバ-,JunDを同定した(平井)。FOS/Jun二量体がCーmycプロモ-タ-の負のエレメントと相互作用することを示した(瀧本)。 RBのリン酸化を抑制するTGFーβによりマウス骨芽細胞で誘導される未知の遺伝子のCDNAを4クロ-ン単離した(野瀬)。E1Aにより発現が促進されるHMG2は超ラセン型DNAを認識する新しいタイプの転写因子であることを示した(吉田)。 2.分裂酵母の細胞周期遺伝子cdc25,wee1に対応するヒト遺伝子を単離解析し、酵母と同称G2制御機能をもつことを示した(岡山)。3YlのG1ts変異株に岡山法CDNAライブラリィを導入し、高温で増殖可能となった細胞を7株単離した(木村)。 3.F9細胞の分化誘導後、Nーmyc発現レベルの低下と対応して、プロモ-タ-で形成される複合体IVも減少することを示した(石橋)。HPV・E6は従来の報告とは異なり、ヒト,ラット細胞ではp53に結合するものゝそれを分解しないことを示した(瀬川)。特定の細胞表面抗原を発現している細胞を選択的に濃縮するパニング法を用い、SV40T抗原誘導ハムスタ-細胞抗原遺伝子のCDNAを単離した(丸山)。SV40tsAでトランスフォ-ムしたヒトTIG3細胞で発現し、その細胞由来の不死化細胞(SVts8)では殆んど発現しない遺伝子のCDNAライブラリィを作製し、特異的なCDNAを数クロ-ン単離した(井出)。

Research Products
(18 results)

All Other

All Publications (18 results)

[Publications] E.Hara: "Mechanism of induction of cellular DNA synthesis by the adenovirus E1A 12S cDNA product." Exp.Cell Res.198. 250-258 (1992)
[Publications] E.Hara: "Subtractive cDNA cloning using oligo(dT)-Latex and PCR:Isolation of cDNA clones specific for undifferentiated human embryonal carinoma cells." Nucl.Acids Res.19. 7097-7104 (1991)
[Publications] E.Hara: "Cooperative effect of antisense-Rb and antisense-p53 oligomers on the extension of life span in human diploid fibroblasts,TIG." Biochem.Biophys.Res.Commun.179. 528-534 (1991)
[Publications] K.Takehana: "Interaction of nuclear factors to the regulatory region of the N-myc gene during differentiation of human embryonal carcinoma cells." Gene. 103. 219-225 (1991)
[Publications] T.Hasegawa: "A transient decrease in N-myc expression and its biological role during differentiation of human embryonal carcinoma cells." Differentiation. 47. 107-117 (1991)
[Publications] T.Yoshida: "Transcription of fra-2 mRNA and phosphorylation of Fra-2 protein are stimulated by serum." Biochem.Biophys.Res.Commun.174. 934-939 (1991)
[Publications] H,Okuno: "Inhibition of jun-transformation by mutated fos gene:Design of an anti-oncogene." Oncogene. 6. 1491-1497 (1991)
[Publications] H.Nishina: "Isolation and characterization of fra-2,an additional member of the fos gene family." Proc.Natl.Acad.Sci.87. 3619-3623 (1990)
[Publications] N.Okazaki: "The ste4+ gene,essential for sexual differentiation of Schizosacharomuces pombe,encodes a protein with a leucine zipper motif." Nucl.Acids Res.19. 7043-7047 (1991)
[Publications] A.Nagata: "An additional homolog of the fission yeast cdc25+ gene occurs in human and is higyly expressed in some cancer cells." The New Biologist. 3. 1-6 (1991)
[Publications] M.Igarashi: "Weel+like gene in human cells." Nature. 353. 80-83 (1991)
[Publications] K.Tanaka: "Analysis of a human DNA excision regair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain." Nature. 348. 73-76 (1990)
[Publications] N.Tsuyama: "SV40 T antigen is required for maintainance of immoratal growth in SV40-transformed human fibroblasts." Cell Struc.Funct.16. 55-62 (1991)
[Publications] M.Nakahara: "Establishment of SV40-transformed cell line from primary culture of rat dorsolateral prostatic epithelial cells." Exp.Cell Res.190. 271-275 (1990)
[Publications] H.Shirakawa: "Structure of a gene coding for human HMG2 protein." J.Biol.Chem.266. (1991)
[Publications] K.Nishimori: "Induction of CAT gene expression on a plasmid vector (L factor) by retinoic acid in mouse embryonal carcinoma (F9) cells." Plasmid. (1991)
[Publications] K.Nose: "Transriptional activation of "early response" genes by hydrogen peroxide in a mouse osteoblastic cell lines." Eur.J.Biochem.201. 99-106 (1991)
[Publications] T.Hirakawa: "Massive accumulation of neutral lipids in cells conditionally transformed by an activated H-ras oncogene." Oncogene. 6. 286-295 (1991)

1991 Fiscal Year Annual Research Report

細胞増殖,分化の制御における核内癌遺伝子の機能

Principal Investigator

小田 鈎一郎 東京理科大学, 基礎工学部, 教授 (40012736)

Research Products

[Publications] E.Hara: "Mechanism of induction of cellular DNA synthesis by the adenovirus E1A 12S cDNA product." Exp.Cell Res.198. 250-258 (1992)

[Publications] E.Hara: "Subtractive cDNA cloning using oligo(dT)-Latex and PCR:Isolation of cDNA clones specific for undifferentiated human embryonal carinoma cells." Nucl.Acids Res.19. 7097-7104 (1991)

[Publications] E.Hara: "Cooperative effect of antisense-Rb and antisense-p53 oligomers on the extension of life span in human diploid fibroblasts,TIG." Biochem.Biophys.Res.Commun.179. 528-534 (1991)

[Publications] K.Takehana: "Interaction of nuclear factors to the regulatory region of the N-myc gene during differentiation of human embryonal carcinoma cells." Gene. 103. 219-225 (1991)

[Publications] T.Hasegawa: "A transient decrease in N-myc expression and its biological role during differentiation of human embryonal carcinoma cells." Differentiation. 47. 107-117 (1991)

[Publications] T.Yoshida: "Transcription of fra-2 mRNA and phosphorylation of Fra-2 protein are stimulated by serum." Biochem.Biophys.Res.Commun.174. 934-939 (1991)

[Publications] H,Okuno: "Inhibition of jun-transformation by mutated fos gene:Design of an anti-oncogene." Oncogene. 6. 1491-1497 (1991)

[Publications] H.Nishina: "Isolation and characterization of fra-2,an additional member of the fos gene family." Proc.Natl.Acad.Sci.87. 3619-3623 (1990)

[Publications] N.Okazaki: "The ste4+ gene,essential for sexual differentiation of Schizosacharomuces pombe,encodes a protein with a leucine zipper motif." Nucl.Acids Res.19. 7043-7047 (1991)

[Publications] A.Nagata: "An additional homolog of the fission yeast cdc25+ gene occurs in human and is higyly expressed in some cancer cells." The New Biologist. 3. 1-6 (1991)

[Publications] M.Igarashi: "Weel+like gene in human cells." Nature. 353. 80-83 (1991)

[Publications] K.Tanaka: "Analysis of a human DNA excision regair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain." Nature. 348. 73-76 (1990)

[Publications] N.Tsuyama: "SV40 T antigen is required for maintainance of immoratal growth in SV40-transformed human fibroblasts." Cell Struc.Funct.16. 55-62 (1991)

[Publications] M.Nakahara: "Establishment of SV40-transformed cell line from primary culture of rat dorsolateral prostatic epithelial cells." Exp.Cell Res.190. 271-275 (1990)

[Publications] H.Shirakawa: "Structure of a gene coding for human HMG2 protein." J.Biol.Chem.266. (1991)

[Publications] K.Nishimori: "Induction of CAT gene expression on a plasmid vector (L factor) by retinoic acid in mouse embryonal carcinoma (F9) cells." Plasmid. (1991)

[Publications] K.Nose: "Transriptional activation of "early response" genes by hydrogen peroxide in a mouse osteoblastic cell lines." Eur.J.Biochem.201. 99-106 (1991)

[Publications] T.Hirakawa: "Massive accumulation of neutral lipids in cells conditionally transformed by an activated H-ras oncogene." Oncogene. 6. 286-295 (1991)

小田鈎一郎東京理科大学, 基礎工学部, 教授 (40012736)