1991 Fiscal Year Annual Research Report

受精の分子機構

Research Project

Project/Area Number	03207103
Research Institution	Tokyo Institute of Technology
Principal Investigator	星元紀東京工業大学, 生命理工学部, 教授 (20012411)
Co-Investigator(Kenkyū-buntansha)	佐藤章福島県立医科大学, 医学部, 教授 (30004948) 豊田裕東京大学, 医科学研究所, 教授 (90050418) 片桐千明北海道大学, 理学部, 教授 (90000827) 新井勇治筑波大学, 応用生物化学系, 教授 (90015636) 森沢正昭東京大学, 理学部, 教授 (40013594)
Keywords	精子運動能 / 受精能獲得 / 先体反応 / アクロシン / 膜融合 / 前核形成 / 自家不稔性 / 卵黄膜
Research Abstract	今年度新に次の様な知見と成果を得た。1.ウニ精子より2種類のプロテアソ-ムを得、運動開始機構との関係を調べた。ニシン卵の精子活性化物質はペプチドであった。(森沢)2.ニジマス卵黄膜第2層に存在する酸性の強い糖タンパク質を単離した。その0ーグリコシドは(ー8KDNα2ー)_<2ー5>であった。この糖タンパク質は同種の精子と特異的に反応した。(井上)3.カタユウレイボヤ卵巣卵の自家不稔性獲得と、濾胞細胞由来のタンニン酸好染性の薄層が卵黄膜最外層に沈着することとがよく対応し、しかもこの層は、ライシンであるキモトリプシン様酵素によって消化される。(星)4.ブタ精子には、透明帯糖タンパク質のAsn結合型中性糖鎖を基質とし得るガラクト-ス転移酵素がある。(高崎)5.マウス精子の頭部細胞質に、プロテインキナ-ゼCアイソザイムのII型が発現している。(鈴木)6.マウスのアクロシンmRNAは、減数分裂期にすでに翻訳されている。(新井)7.抗IP_3モノクロ-ン抗体を用い、ハムスタ-卵の受精時にIP_3依存Ca^<2++>遊離機構が働くことを証明した。(宮崎)8.先体反応後のヒト精子に対するモノクロ-ン抗体を磁気ビ-ズに結合し、精子の体外受精能検定法を確立した。(岡部)9.細胞性粘菌配偶子の融合を阻止するモノクロ-ン抗体の抗原を解析し、細胞表面糖鎖の重要性を見出した。(漆原)10.カエル精子核塩基性タンパクの離脱は、卵細胞質のヌクレオプラスミンによる。(片桐)11.中心体の分離と機能的な双極紡錘体の形成にはMPF活性が関わっている。(岩尾)12.ブタ卵胞液中で成熟した卵のH1キナ-ゼ活性は、基礎培地中で成熟させたものより高い。初期胚の性判別法を開発した。(豊田)13.哺乳類の高温処理精子核は卵に注入後前核を形成した。ウサギデは高温処理精子核を卵に注入すると、6ー8細胞期にまで発生した。(佐藤)14.マウス未成熟輸卵管の器官培養系を用いマウス受精卵を胚盤胞まで発生させた。(内貴)

Research Products
(28 results)

All Other

All Publications (28 results)

[Publications] T.Yasuhara: "Involvement of a Sperm Aminopeptidase in Fertilization of the Sea Urchin" Experientia. 47. 100-103 (1991)
[Publications] H.Tadenuma: "Purification and Characterization of a GTP-Binding Protein Serving as Pertussin Toxin Substrate in Starfish Oocytes" Arch.Biochem.Biophys.290. 411-417 (1991)
[Publications] E.Sato: "A New Fluorogenic Substrate with Pyranine System for Phosphatases" Chem.Pharm.Bull.
[Publications] T.Amano: "Treatment of Starfish Sperm with Egg Jelly Induces the Degradation of Histones" Develop.Growth,Differ.34. 99-106 (1992)
[Publications] M.Takagi-Sawada: "Participation of 650-kDa Protease (20S Proteasome) in Starfish Oocyte Maturation" Develop.Biol.150. (1992)
[Publications] M.Ogiso: "Senile Cataract-Related Accumulation of Lewis^x Glycolipid in Human Lens" J.Biol.Chem.(1992)
[Publications] Suzuki,O.: "In vitro development of mouse emkryos from various strains" Proc.Jpn.Soc.Anim.Biochem. 27. 7-12 (1991)
[Publications] Miyazaki,S.: "Repotitive calcium transients in hamster ooeytes" Cell Calcium. 12. 205-216 (1991)
[Publications] Miyazaki,S.: "Intracellular calcium release mechanism in hamster eggs." Biomed.Res.12. 185-187 (1991)
[Publications] Iwao,Y.: "Control of sperm nuclear behavior in physialogically polyspermio new eggs:possible in nolvement of MPF." Develop.Biol.142. 301-312 (1990)
[Publications] Habata,Y.: "Possible existence of a light-inducible protein that inhibits sexual cell fusion in Dictyostelium discoedeum." Cell Struct.Funct.16. 185-187 (1991)
[Publications] Ishikawa,T.: "Involvement of cell surface carbohydrate in the sexual cell fusion of Dictyostelium discoideum." Develop.Growth and Differ. 33. 131-137 (1991)
[Publications] Yanagida,K.: "Cleanage of rabbit eggs after microsurgical injection of testicular spermatozoa." Hum.Reprod.6. 269-277 (1991)
[Publications] Hoshi,K.: "Importance of Ca^<++> K^+ and glucose in the medium for sperm ponotraction through the human zona pellucida" J.Exp.Med.165. 99-104 (1991)
[Publications] Yamasaki,H.: "Egg exudata-induced reduction of sperm lysin sensitivity in the vitelline coat after fertilization of Bubo japonicus and its participation in polyspermny block." J.Exp.Zool.258. 404-413 (1991)
[Publications] Yokota,T.: "Nuclear basic proteins of Xenopus laevis sperm:their characterization and synthesis during spermatogenesis." Develop.Growth.Differ. 33. 19-27 (1991)
[Publications] Takamune,K.: "Primary structure of toad sperm protamines and nucleatide sequence of their cDNAs." Eur.J.Biochem. 196. 401-406 (1991)
[Publications] Ohsumi,K.: "Characterization of the ooplasmic factor inducing decondensation of and protamine removal from toad sperm nucle involvement of nucleaplasmin" Develop.Biol.,. 148. 295-305 (1991)
[Publications] R.Marino: "Purification and Characterization of A Vitelline Coat Lysin from Ciona intestinalis Spermatozoa" Mol.Reprod.Develop.(1992)
[Publications] H.Kubo: "A Novel Ceramide Trihexoside from the Eggs of the Sea Urchin,Hemicentrotus pulcherrimus" J.Biochem.(1992)
[Publications] H.Kubo: "A Novel Difucosylated Neutral Glycosphingolipid from the Eggs of the Sea Urchin,Hemicentrotus pulcherrimus I.Purification and Structural Determination" J.Biochem.
[Publications] F.Inagaki: "A Novel Difucosylated Neutral Glycosphingolipid from the Eggs of the Sea Urchin,Hemicentrotus pulcherrimus II.Structural Analysis by Two Dimensional Nuclear Magnetic Resonance" J.Biochem.
[Publications] H.Sawada: "Inhibition of Mouse Blastocyst Hatching by Subsite-Specific Trypsin Inhibitors,Peptidyl-Argininals" Develop.,Growth,Differ.
[Publications] R.De Santis: "Metalloendoproteases Are Involved in Gamete Fusion of Ciona intestinalis" Develop.Biol.
[Publications] Urushihara,H.: "Isolation and characterization of Dictyostelium mutanta defective in sexial cell fusion." Develop.growth & Differ. 33. 517-523 (1991)
[Publications] Okabe,M.: "A human sperm antigen possibly involved in binding and/or fusion with zona-free hamster eggs." Fertil Steril. 54. 1121-1126 (1990)
[Publications] M.Hoshi: "“Comparative Spermatology,20 Years After" (Serono Symposia Publication Series,Vol.75)" Raven Press,New York, (1992)
[Publications] M.Hoshi: "“Biology of the Germ Line -In Animals and Man-"" Japan Scientific Societies Press,Tokyo, (1992)

1991 Fiscal Year Annual Research Report

受精の分子機構

Principal Investigator

星 元紀 東京工業大学, 生命理工学部, 教授 (20012411)

Research Products

[Publications] T.Yasuhara: "Involvement of a Sperm Aminopeptidase in Fertilization of the Sea Urchin" Experientia. 47. 100-103 (1991)

[Publications] H.Tadenuma: "Purification and Characterization of a GTP-Binding Protein Serving as Pertussin Toxin Substrate in Starfish Oocytes" Arch.Biochem.Biophys.290. 411-417 (1991)

[Publications] E.Sato: "A New Fluorogenic Substrate with Pyranine System for Phosphatases" Chem.Pharm.Bull.

[Publications] T.Amano: "Treatment of Starfish Sperm with Egg Jelly Induces the Degradation of Histones" Develop.Growth,Differ.34. 99-106 (1992)

[Publications] M.Takagi-Sawada: "Participation of 650-kDa Protease (20S Proteasome) in Starfish Oocyte Maturation" Develop.Biol.150. (1992)

[Publications] M.Ogiso: "Senile Cataract-Related Accumulation of Lewis^x Glycolipid in Human Lens" J.Biol.Chem.(1992)

[Publications] Suzuki,O.: "In vitro development of mouse emkryos from various strains" Proc.Jpn.Soc.Anim.Biochem. 27. 7-12 (1991)

[Publications] Miyazaki,S.: "Repotitive calcium transients in hamster ooeytes" Cell Calcium. 12. 205-216 (1991)

[Publications] Miyazaki,S.: "Intracellular calcium release mechanism in hamster eggs." Biomed.Res.12. 185-187 (1991)

[Publications] Iwao,Y.: "Control of sperm nuclear behavior in physialogically polyspermio new eggs:possible in nolvement of MPF." Develop.Biol.142. 301-312 (1990)

[Publications] Habata,Y.: "Possible existence of a light-inducible protein that inhibits sexual cell fusion in Dictyostelium discoedeum." Cell Struct.Funct.16. 185-187 (1991)

[Publications] Ishikawa,T.: "Involvement of cell surface carbohydrate in the sexual cell fusion of Dictyostelium discoideum." Develop.Growth and Differ. 33. 131-137 (1991)

[Publications] Yanagida,K.: "Cleanage of rabbit eggs after microsurgical injection of testicular spermatozoa." Hum.Reprod.6. 269-277 (1991)

[Publications] Hoshi,K.: "Importance of Ca^<++> K^+ and glucose in the medium for sperm ponotraction through the human zona pellucida" J.Exp.Med.165. 99-104 (1991)

[Publications] Yamasaki,H.: "Egg exudata-induced reduction of sperm lysin sensitivity in the vitelline coat after fertilization of Bubo japonicus and its participation in polyspermny block." J.Exp.Zool.258. 404-413 (1991)

[Publications] Yokota,T.: "Nuclear basic proteins of Xenopus laevis sperm:their characterization and synthesis during spermatogenesis." Develop.Growth.Differ. 33. 19-27 (1991)

[Publications] Takamune,K.: "Primary structure of toad sperm protamines and nucleatide sequence of their cDNAs." Eur.J.Biochem. 196. 401-406 (1991)

[Publications] Ohsumi,K.: "Characterization of the ooplasmic factor inducing decondensation of and protamine removal from toad sperm nucle involvement of nucleaplasmin" Develop.Biol.,. 148. 295-305 (1991)

[Publications] R.Marino: "Purification and Characterization of A Vitelline Coat Lysin from Ciona intestinalis Spermatozoa" Mol.Reprod.Develop.(1992)

[Publications] H.Kubo: "A Novel Ceramide Trihexoside from the Eggs of the Sea Urchin,Hemicentrotus pulcherrimus" J.Biochem.(1992)

[Publications] H.Kubo: "A Novel Difucosylated Neutral Glycosphingolipid from the Eggs of the Sea Urchin,Hemicentrotus pulcherrimus I.Purification and Structural Determination" J.Biochem.

[Publications] F.Inagaki: "A Novel Difucosylated Neutral Glycosphingolipid from the Eggs of the Sea Urchin,Hemicentrotus pulcherrimus II.Structural Analysis by Two Dimensional Nuclear Magnetic Resonance" J.Biochem.

[Publications] H.Sawada: "Inhibition of Mouse Blastocyst Hatching by Subsite-Specific Trypsin Inhibitors,Peptidyl-Argininals" Develop.,Growth,Differ.

[Publications] R.De Santis: "Metalloendoproteases Are Involved in Gamete Fusion of Ciona intestinalis" Develop.Biol.

[Publications] Urushihara,H.: "Isolation and characterization of Dictyostelium mutanta defective in sexial cell fusion." Develop.growth & Differ. 33. 517-523 (1991)

[Publications] Okabe,M.: "A human sperm antigen possibly involved in binding and/or fusion with zona-free hamster eggs." Fertil Steril. 54. 1121-1126 (1990)

[Publications] M.Hoshi: "“Comparative Spermatology,20 Years After" (Serono Symposia Publication Series,Vol.75)" Raven Press,New York, (1992)

[Publications] M.Hoshi: "“Biology of the Germ Line -In Animals and Man-"" Japan Scientific Societies Press,Tokyo, (1992)

星元紀東京工業大学, 生命理工学部, 教授 (20012411)