1991 Fiscal Year Annual Research Report

植物遺伝子発現の柔軟性

Research Project

Project/Area Number	03262103
Research Institution	The University of Tokyo
Principal Investigator	長田敏行東京大学, 理学部, 教授 (10012519)
Co-Investigator(Kenkyū-buntansha)	岡田清孝岡崎国立共同研究機構, 基礎生物学研究所, 助教授 (50101093) 福田裕穂東北大学, 理学部, 助教授 (10165293) 中村研三名古屋大学, 農学部, 助教授 (80164292) 庄野邦彦東京大学, 教養学部, 教授 (60050457) 大野哮司北海道大学, 農学部, 教授 (00011726)
Keywords	植物ホルモン / 遺伝子発現制御 / 転写因子 / 多重遺伝子族 / 細胞分化 / オ-キシン / アブシジン酸 / シロイヌナズナ
Research Abstract	植物における遺伝子発現の柔軟性の見地から興味あるのは、植物ホルモンを含む因子群により遺伝子発現制御の動態はいかなるものかという点にある。この点から研究発足の初年度にあたり、基礎固めをすべく植物ホルモンとしてはオ-キシンなどに着目した解析を行なったところオ-キシンにより細胞周期のGoーS期に密接に関連して発現する遺伝子の一群parA,parB,parCが誘導されることがわかったが、parBはグルタチオンSートラスフェラ-ゼ遺伝子であった。これは、オ-キシンで誘導される遺伝子の中で機能が同定された最初の例となり、その他は一群の遺伝子ファミリ-を構成し、様々なシグナルに様々に応答することが判明し、標記の目的に好適な系であることが判明した。また、シロイヌナズナで花芽形成の変異を示す突然変異はオ-キシンの輸送に変異があることも示され、このところ研究の停滞していたオ-キシン研究に突破口を開いた。一方、蔗糖およびポリガラクツロン酸に応答することが報告されているベ-タアミラ-ゼの発現に関するシス、トランスの因子の解析が進み遺伝子発現は確かに柔軟といってよい状況にあることが示され、より詳細な機構の解析が待たれる状況でもある。多重遺伝子族の例としてはフェニルアラニンアンモニアリア-ゼが、傷害により誘導されるものがあるとともにオ-キシンで誘導されるものがあることが分かり、その使い分けの分子機構も詳しく知りたいところである。更に、ヒャクニチソウ葉肉細胞で見られる仮導管要素の分化でも関与する遺伝子群が判明しつつあることも標記の課題にある解答を準備する状況といえる。班全体としてはなお目標にむけ鋭意邁進する必要であるが、明らかにされた成果には見るべきものがあると考える。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Y.Takahashi: "Characterization of the auxin-regulated par gene from tobacco mesophyll protoplasts." The Plant Journal. 1. 327-332 (1991)
[Publications] Y.Takahashi: "ParB:An auxin-regulated gene encoding glutathione S-transferase" Proc.Natl.Acad.Sci.USA. 89. 56-59 (1992)
[Publications] S.Ohta: "Stem-predominant expression of the sweet potato sporamin gene promoter:beta-glucuronidase (GUS) fusion gene in transgenic tobacco is conferred by multiple cell type-specific regulatory elements." Molecular & General Genetics. 225. 369-378 (1991)
[Publications] K.Matsuoka: "Propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting." Proc.Natl.Acad.Sci.USA. 88. 834-838 (1991)
[Publications] M.Ishikawa: "Isolation of mutants of Arabidopsis thaliana in which accumulation of tobacco mosaic virus coat protein is reduced to low levels." Molecular & General Genetics. 230. 33-38 (1991)
[Publications] K.Terada: "Site-directed mutagenesis of the conserved histidine residue of phosphenolpyruvate carboxylase:His 138 is essential for the second partial reaction." European Journal of Biochemistry. (1992)
[Publications] T.Fujita: "Changes in moropology,levels of endogenous IAA and protein composition in relation to the devlopment of tobacco genetic tumor induced in the dark." Plant and Cell Physiology. 32. 169-177 (1991)
[Publications] M.Kawaguchi: "The presence of an enzyme that converts indole-3-acetic actamide into IAA in wild and cultivated rice." Plant and Cell Physiology. 32. 143-149 (1991)
[Publications] H.Fukuda: "Tracheary element formation as a model system of cell defferentiation.." International Review of Cytology. 132. (1992)
[Publications] K.Okada: "Requirement of the auxin polar transport in Arabodopsis floral bud formation." The Plant Cell. 3. 677-684 (1991)
[Publications] K.Toriyama: "A Brassica S locus gene promoter directs sporophytic expression in the anther tapetum of transgenic Arabidopsis." Developmental Biology. 143. 427-431 (1991)
[Publications] K.Toriyama: "Transformation of Brassica oleracea with an S-locus gene from B.campestris changes the self-incompatibility phenotype." Theoretical & Applied Genetics. 81. 769-776 (1991)
[Publications] Y.Li: "Nucleic acid-binding specificities of tobacco chloroplast ribonuceoproteins." Nucleic Acids Research. 19. 2893-2896 (1991)
[Publications] M.Senda: "Genomic organization and sequence analysis of the cytochrome oxidase subunit II gene from normal and male sterile mitochondria in sugar beat." Current Genetics. 19. 175-181 (1991)
[Publications] Y.Nagano: "Nucleotide sequense and expression of the ribosomal protein L2 gene in pea chloroplasts." Plant Molecular Biology. 17. 541-545 (1991)
[Publications] Y.Nagano: "Sequence and transcriptional analysis of the cluster trnQ-zfpA-psaI-ORF231-petA in pea chloroplasts." Current Genetics. 20. 431-436 (1991)
[Publications] A.Iida: "Efficiency of particle-bombardment mediated transformation is influenced by cell cycle stage in synchronized cultured cells of tobacco." Plant Physiology. 97. 1585-1587 (1991)
[Publications] T.Yamashita: "Evidence that over 90% of beat-glucuronidase-expressing cells after particle bombardment directly receive the foreign in their nucleus." Plant Physiology. 97. 829-831 (1991)

1991 Fiscal Year Annual Research Report

植物遺伝子発現の柔軟性

Principal Investigator

長田 敏行 東京大学, 理学部, 教授 (10012519)

Research Products

[Publications] Y.Takahashi: "Characterization of the auxin-regulated par gene from tobacco mesophyll protoplasts." The Plant Journal. 1. 327-332 (1991)

[Publications] Y.Takahashi: "ParB:An auxin-regulated gene encoding glutathione S-transferase" Proc.Natl.Acad.Sci.USA. 89. 56-59 (1992)

[Publications] S.Ohta: "Stem-predominant expression of the sweet potato sporamin gene promoter:beta-glucuronidase (GUS) fusion gene in transgenic tobacco is conferred by multiple cell type-specific regulatory elements." Molecular & General Genetics. 225. 369-378 (1991)

[Publications] K.Matsuoka: "Propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting." Proc.Natl.Acad.Sci.USA. 88. 834-838 (1991)

[Publications] M.Ishikawa: "Isolation of mutants of Arabidopsis thaliana in which accumulation of tobacco mosaic virus coat protein is reduced to low levels." Molecular & General Genetics. 230. 33-38 (1991)

[Publications] K.Terada: "Site-directed mutagenesis of the conserved histidine residue of phosphenolpyruvate carboxylase:His 138 is essential for the second partial reaction." European Journal of Biochemistry. (1992)

[Publications] T.Fujita: "Changes in moropology,levels of endogenous IAA and protein composition in relation to the devlopment of tobacco genetic tumor induced in the dark." Plant and Cell Physiology. 32. 169-177 (1991)

[Publications] M.Kawaguchi: "The presence of an enzyme that converts indole-3-acetic actamide into IAA in wild and cultivated rice." Plant and Cell Physiology. 32. 143-149 (1991)

[Publications] H.Fukuda: "Tracheary element formation as a model system of cell defferentiation.." International Review of Cytology. 132. (1992)

[Publications] K.Okada: "Requirement of the auxin polar transport in Arabodopsis floral bud formation." The Plant Cell. 3. 677-684 (1991)

[Publications] K.Toriyama: "A Brassica S locus gene promoter directs sporophytic expression in the anther tapetum of transgenic Arabidopsis." Developmental Biology. 143. 427-431 (1991)

[Publications] K.Toriyama: "Transformation of Brassica oleracea with an S-locus gene from B.campestris changes the self-incompatibility phenotype." Theoretical & Applied Genetics. 81. 769-776 (1991)

[Publications] Y.Li: "Nucleic acid-binding specificities of tobacco chloroplast ribonuceoproteins." Nucleic Acids Research. 19. 2893-2896 (1991)

[Publications] M.Senda: "Genomic organization and sequence analysis of the cytochrome oxidase subunit II gene from normal and male sterile mitochondria in sugar beat." Current Genetics. 19. 175-181 (1991)

[Publications] Y.Nagano: "Nucleotide sequense and expression of the ribosomal protein L2 gene in pea chloroplasts." Plant Molecular Biology. 17. 541-545 (1991)

[Publications] Y.Nagano: "Sequence and transcriptional analysis of the cluster trnQ-zfpA-psaI-ORF231-petA in pea chloroplasts." Current Genetics. 20. 431-436 (1991)

[Publications] A.Iida: "Efficiency of particle-bombardment mediated transformation is influenced by cell cycle stage in synchronized cultured cells of tobacco." Plant Physiology. 97. 1585-1587 (1991)

[Publications] T.Yamashita: "Evidence that over 90% of beat-glucuronidase-expressing cells after particle bombardment directly receive the foreign in their nucleus." Plant Physiology. 97. 829-831 (1991)

長田敏行東京大学, 理学部, 教授 (10012519)