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1992 Fiscal Year Final Research Report Summary

The structure and function of the enzymes concerned with glycolysis

Research Project

Project/Area Number 03303014
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 結晶学
Research InstitutionNagoya University

Principal Investigator

YAMANE Takashi  Nagoya University,School of Engineering, Associate Professor, 工学部, 助教授 (80030055)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Osamu  Tokyo Institute of Technology, Faculty of Bioscience and Biotechnology, Res. Ass, 生命理工学部, 助手 (10231599)
HARADA Shigeharu  Osaka University, Faculty of Engineering, Assistant Professor, 工学部, 講師 (80156504)
FUKUYAMA Keiichi  Osaka University, Faculty of Science, Assoc. Prof., 理学部, 助教授 (80032283)
MATSUURA Yoshiki  Osaka University, Institute for Protein Research, Assoc. Prof., 蛋白質研究所, 助教授 (90029968)
IIJIMA Shinji  Nagoya University, School of Engineering, Professor, 工学部, 教授 (00168056)
Project Period (FY) 1991 – 1992
Keywordsalpha-amylase / lysozyme / malto-tetraose(G4) forming amylase / malto-pentaose(G5) forming amylase / beta-galactosidase / cellobiohydrolase / crystal structure / molecular replacement
Research Abstract

The crystal structure analyses of the enzymes concerned with glycolysis have been carried out during 1991-1992 with the financial support of this Grant-in-Aid. The molecular structures of xylanase and cellulase by Dr. Hata, malto-teraose(G4) forming exo-amylase by Dr. Matsuura, bacterial lysozyme by Dr. Harada, were determined. The functions of objective enzymes were made clear on the basis of their structures. The advanced crystallographic studies were promoted on bacterial alpha-amylases by Dr. Yamane, alpha-amylase inhibitor by Dr. Fukuyama, and malto-pentaose (G5) forming amylase by Dr. Hamada, etc. The crystallization techniques of proteins were also improved, for example, by Drs. Harada and Highchi. For the structure analysis, the methodology on the molecular replacement method was made a great advance by Drs. Yamane, Tanaka, etc. and the know-how was published in the collective research reports. The improvement of the hardwares and softwares to collect and process the X-ray data more rapidly was carried out by Prof. Satow and Drs. Tanaka and Morimoto. The cloning of beta-galactosidase and cellobiohydrolase in prokaryotic cells could be executed by Prof. Iijima, in order to use biomass energy resouces effectively.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] A.Suzuki,T.Nishio,C.Yamamoto,T.Yamane,T.Ashida: "Crystal structure of Bacillus amyloliquefaciens α-amylase"

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiki Matsuura: "AUTOMR:an automatic processing program system for the molecular replacement method" J.Appl.Cryst.24. 1063-1066 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Morishita,Y.Matsuura,M.Kubota,M.Sato,S.Sakai,Y.Katsube: "Crystal structure of the catalytic domain of the maltotetraose forming exo-amylase from P.stutzeri."

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Matsuura,M.Kusunoki,M.Kakudo: "Structure and catalytic implications of Taka-amylase A" Denpun Kagaku. 38. 137-139 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 久保田 倫夫,勝部 幸輝,松浦 良樹,堺 修造: "シクロデキストリン合成酵素の立体構造と基質結合部位解析" 澱粉科学. 38. 141-146 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Longyin Chen: "Crystal structure of an electron-transfer complex between methylamine dehydrogenase and amicyanin" Biochemistry. 31. 4959-4964 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Suzuki,T.Nishio,C.Yamamoto,T.Yamane,T.Ashida and S.Udaka: "Crystal structure of Bacillus amyloliquefaciens-amylase." J.Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiki Matsuura: "AUTOMR: an automatic processing program system for the molecular replacement method." J.Appl.Cryst.24. 1063-1066 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Matsuura,M.Kusunoki and M.Kakudo: "Structure and catalytic implications of Takaamylase A." Denpun Kagaku. 38. 137-139 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Longyin Chen: "Crystal structure of an electron-transfer complex between methylamine dehydrogenase and amicyanin." Biochemistry. 31. 4959-4964 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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