| Project/Area Number |
03304001
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
遺伝学
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| Research Institution | UNIVERSITY OF TSUKUBA |
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Principal Investigator |
TAKAHASHI Mihoko Univ.Tsukuba, Inst.Biol.Sci., Associate Professor, 生物科学系, 助教授 (90006453)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKII Yuuji Hosei Univ., Fac.Gen.Educ. Professor, 第一教養部, 教授 (20163777)
NUMATA Osamu Univ.Tsukuba, Inst.Biol., Associate Professor, 生物科学系, 助教授 (50189354)
MIKAMI Kazuyuki Miyagi Univ.Ed., Fac.Educ., Associate Professor, 教育学部, 助教授 (90091777)
FUJISHIMA Masahiro Yamaguti Univ., Fac.Sci., Professor, 理学部, 教授 (40127783)
TAKAGI Yoshiomi Nara Woman's Univ., Fac.Sci., Associate Professor, 理学部, 助教授 (90079682)
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| Project Period (FY) |
1991 – 1993
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| Keywords | Ciliates / Nuclear differentiation / Macronucleus / Micronucleus / Mating-type substances / Filament forming protein / Multi-functional protein / Michondrial plasmid |
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| Research Abstract |
1. Main results obtained from the analyzes of the developmental processes.
(1) Mic division inducing facor (mic DIF) and suppressor for the mic DIF were found expressed at very distict stage during conjugation process, . Heat treatment at early stage of meiosis induced an arrest caused by the factors. (2) Monoclonal antibodies against a macronucleus were found to recognize a nucleolar protein. Monoclonal antibodies which recognize the micronucleus during meiosis, possible mating type substance V and cilia located at oral side were isolated. (3) ParGF(Paramecium grouth factor) which rescues normal growth rate of jumyo mutant exhibited functional similarity to human fetal serum. (4) The 14nm filament which has been though to be involving in the formation of gametic nuclei, was identified as the mitochondrial citrate synthetase. A new protein which copurifies with the 14nm filament was isolated and identified as the elongation factor-1alpha. (5) A variant with short immaturity period had a short circadian rhythm. A corelation between circadian rhythm and phisiologica activity was observed. Mature cells injected with DNA from immature cells become immature. This suggests that a developmental clock itself involves a change of DNA during development. (6) Conversions of syngen specificity of the mating type controlling genes were discovered by intersyngenic macronucleoplasmic transplantation.
2. Development of transformation or cloning of mutated genes. (1) DraI fragments more than 10kb were effective to restore the tnd2 mutants when digested DNA fragments from wild type were introduced to mutant cells. If the proper enzyme will be found, cloning of tnd2 gene may be possible. (2) Tubingen3, the vector derived from stylonychia can be used for both Tetrahymena and Paramecium. (3) Non-bacterial inclusion bodies and plasmid-like DNA in mitochondria were found in Paramecium. By DNA analyzes, the plasmid-like DNAs were deviding to three groups.
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