1993 Fiscal Year Annual Research Report

筋形成の分子機構

Research Project

Project/Area Number	03304008
Research Institution	Chiba University
Principal Investigator	大日方昂千葉大学, 理学部, 教授 (40012413)
Co-Investigator(Kenkyū-buntansha)	眞崎知生京都大学, 医学部, 教授 (60009991) 鍋島陽一国立精神神経センター神経研, 部長 (60108024) 佐藤矩行京都大学, 理学部, 助教授 (30025481) 浅島誠東京大学, 教養学部, 教授 (00090564) 嶋田裕医学部, 教授 (70009116)
Keywords	筋分化 / 骨格筋細胞 / 中胚葉誘導 / MyoD / 転写制御 / 筋原線維形成 / 筋蛋白質 / 細胞系譜
Research Abstract	筋形成の一連の過程(筋細胞系列の決定、分化誘導、筋蛋白質遺伝子発現、収縮構造形成)の分子機構について研究を進め、以下の成果をあげた。 1)中胚葉誘導因子アクチビンの濃度依存的な誘導と誘導される側の部域と発生段階特性を詳細に解明、さらにツメガエルの筋分化因子Myf5の発現制御を解析した(浅島)。 2)筋分化制御因子であるMyogenin遺伝子の欠失マウスをジーンターゲット法により作成、筋形成への影響を探り、Myogeninの欠失でも筋芽細胞は生ずるが成熟した筋細胞に至らないことを見いだした(鍋島)。 3)ホヤの5種の筋肉アクチン遺伝子クラスターおよびそれらの発現特性を解明した(佐藤)。 4)筋細胞の分化/脱分化におけるc-jun、cdk2/cyclin、cdc2/cyclin、Rbの活性化と不活性化の相関を明らかにした(遠藤)。 4)筋細胞株(C2)をマウスに注入、生体内で分化へ導く実験系をつくった(小沢)。 5)収縮蛋白質の構造への集合について。形成期の筋で蛋白質集合制御に関わるプロフィリン(大日方)、115kDa αアクニチン(真崎)、C-蛋白質(心筋型)のcDNAを分離、全構造を決定した。アクチン制御蛋白質コフィリンが筋萎縮時に高発現することを見いだした(大日方)。ビチオン標識アクチンを培養心筋細胞に注入、アクチンの筋原繊維への集合過程を電子顕微鏡で解明した(嶋田)。

Research Products
(23 results)

All Other

All Publications (23 results)

[Publications] Obinata,Takashi: "Contractile proteins and myofibrillogenesis." Internatl.Rev.Cytol. 143. 153-189 (1993)
[Publications] Nakae,Hiroki: "Ascidian entactin/nidogen:Implication of evolution by shuffiling two kinds of cysteine-repeats." Eur.J.Biochem.213. 11-19 (1993)
[Publications] Shinagawa,Yoko: "Increased expression of cofilin in denervated chicken skeletal muscle." Zoll.Sci.10. 611-618 (1993)
[Publications] Hayakawa,Kimihide: "Increased expression of cofilin in dystrophic chicken and mouse skeletal muscles." J.Biochem.114. 582-589 (1993)
[Publications] Ono,Shoichiro: "Characterization of novel cofilin isoform which is predominantly expressed in mammalian skeletal muscle." J.Biol.Chem.(in press). (1994)
[Publications] Maruyama,K: "A novel domain sequence of connectin localized at the I band of skeletal muscle sarcomeres:homology to neurofilament subunits." Biochem.Biophys.Res.Commun.194. 1288-1291 (1993)
[Publications] Saitoh,O.: "Expression of myogenic factors in denerved chicekn breast muscle isolation of the chicken Myf5 gene." Nucleic Acids Res.21. 2503-2509 (1993)
[Publications] Imamura,M.: "Identification of a 115-kDa protein from muscle tissues:Expression of a novel nonmuscle α-actinin in vascular endothelial cells" Exp.Cell Res.(in press). (1994)
[Publications] Yamamoto,H.: "Heterogeneity of dystrophin-associated protein." J.Biochem.114. 132-139 (1993)
[Publications] Yoshida,M.: "A dystorphin-associated glycoprotein,A3a(one of 43DAG doublets)is retaind in Duchenne muscular dystrophy muscle." J.Biochem.114. 634-639 (1993)
[Publications] Mizuno,Y.: "Expression of utrophin(dystrophin-related protein)and dystrophin-associated glycoproteins in muscles from patients..." Muscle Nerve. 17. 206-216 (1994)
[Publications] Kinoshita,K.: "Competence prepattern in the animal hemisphere of the 8-cell-stage.Xenopus embryo." Develop.Biol.160. 276-284 (1993)
[Publications] Uchiyama,H.: "Immunohistochemistry of activin and follistatin." The Journal of Histochemistry and Cytochemistry. (in press). (1994)
[Publications] Hirosaka,A.: "Regulated spatial expression of fusion gene constructs with the 5' upstream region of Halocynthia roretzi muscle actin gene..." Roux's Arch.Dev.Biol.203. 104-112 (1993)
[Publications] Araki,I.: "Expression of AMD1,a gene for a MyoD1-related factor in the ascidian Halocynthia reretzi." Roux's Arch.Dev.Biol.(in press). (1994)
[Publications] Asakuma,A.: "MyoD and myogenin act on the chicken myosin light chain 1 gene as distince transcription factors." Mol.Cell.Biol.13. 7153-7162 (1993)
[Publications] Nabeshima,Y.: "Myogenin gene disruption results in perinatal lethality because of severe muscle defect." Nature. 364. 532-535 (1993)
[Publications] Fukami,K.: "α-actinin and vinculin are PIP2-binding proteins involved in signaling by tyrosine kinase." J.Biol.Chem.269(in press). (1994)
[Publications] Maruyama,K.: "A partial connectin cDNA encoding a novel type of RSP motifs isolated from chicken embryonic skeletal muscle." J.Biochem.115(in press). (1994)
[Publications] Komiyama,M.: "Dynamics of actin and assembly of connectin(titin)during myofibrillogenesis in embryonic chick cardiac muscle cells in vitro" Dev.Dyn.196. 291-299 (1993)
[Publications] Atsuta,F.: "Distribution of connectin(titin),nebulin and α-actinin at myotendinous junctions of chicken pectoralis muscles:....." J.Muscle Res.Cell Motil.14. 511-517 (1993)
[Publications] Toyota,N.: "Expression of troponin C genes during development in the chicken." Int.J.Dev.Biol.(in press). (1994)
[Publications] Fukui,A.: "Isolation and characterization of xenopus follistatin and activins." Developmental Biology. 159. 131-139 (1993)

1993 Fiscal Year Annual Research Report

筋形成の分子機構

Principal Investigator

大日方 昂 千葉大学, 理学部, 教授 (40012413)

Research Products

[Publications] Obinata,Takashi: "Contractile proteins and myofibrillogenesis." Internatl.Rev.Cytol. 143. 153-189 (1993)

[Publications] Nakae,Hiroki: "Ascidian entactin/nidogen:Implication of evolution by shuffiling two kinds of cysteine-repeats." Eur.J.Biochem.213. 11-19 (1993)

[Publications] Shinagawa,Yoko: "Increased expression of cofilin in denervated chicken skeletal muscle." Zoll.Sci.10. 611-618 (1993)

[Publications] Hayakawa,Kimihide: "Increased expression of cofilin in dystrophic chicken and mouse skeletal muscles." J.Biochem.114. 582-589 (1993)

[Publications] Ono,Shoichiro: "Characterization of novel cofilin isoform which is predominantly expressed in mammalian skeletal muscle." J.Biol.Chem.(in press). (1994)

[Publications] Maruyama,K: "A novel domain sequence of connectin localized at the I band of skeletal muscle sarcomeres:homology to neurofilament subunits." Biochem.Biophys.Res.Commun.194. 1288-1291 (1993)

[Publications] Saitoh,O.: "Expression of myogenic factors in denerved chicekn breast muscle isolation of the chicken Myf5 gene." Nucleic Acids Res.21. 2503-2509 (1993)

[Publications] Imamura,M.: "Identification of a 115-kDa protein from muscle tissues:Expression of a novel nonmuscle α-actinin in vascular endothelial cells" Exp.Cell Res.(in press). (1994)

[Publications] Yamamoto,H.: "Heterogeneity of dystrophin-associated protein." J.Biochem.114. 132-139 (1993)

[Publications] Yoshida,M.: "A dystorphin-associated glycoprotein,A3a(one of 43DAG doublets)is retaind in Duchenne muscular dystrophy muscle." J.Biochem.114. 634-639 (1993)

[Publications] Mizuno,Y.: "Expression of utrophin(dystrophin-related protein)and dystrophin-associated glycoproteins in muscles from patients..." Muscle Nerve. 17. 206-216 (1994)

[Publications] Kinoshita,K.: "Competence prepattern in the animal hemisphere of the 8-cell-stage.Xenopus embryo." Develop.Biol.160. 276-284 (1993)

[Publications] Uchiyama,H.: "Immunohistochemistry of activin and follistatin." The Journal of Histochemistry and Cytochemistry. (in press). (1994)

[Publications] Hirosaka,A.: "Regulated spatial expression of fusion gene constructs with the 5' upstream region of Halocynthia roretzi muscle actin gene..." Roux's Arch.Dev.Biol.203. 104-112 (1993)

[Publications] Araki,I.: "Expression of AMD1,a gene for a MyoD1-related factor in the ascidian Halocynthia reretzi." Roux's Arch.Dev.Biol.(in press). (1994)

[Publications] Asakuma,A.: "MyoD and myogenin act on the chicken myosin light chain 1 gene as distince transcription factors." Mol.Cell.Biol.13. 7153-7162 (1993)

[Publications] Nabeshima,Y.: "Myogenin gene disruption results in perinatal lethality because of severe muscle defect." Nature. 364. 532-535 (1993)

[Publications] Fukami,K.: "α-actinin and vinculin are PIP2-binding proteins involved in signaling by tyrosine kinase." J.Biol.Chem.269(in press). (1994)

[Publications] Maruyama,K.: "A partial connectin cDNA encoding a novel type of RSP motifs isolated from chicken embryonic skeletal muscle." J.Biochem.115(in press). (1994)

[Publications] Komiyama,M.: "Dynamics of actin and assembly of connectin(titin)during myofibrillogenesis in embryonic chick cardiac muscle cells in vitro" Dev.Dyn.196. 291-299 (1993)

[Publications] Atsuta,F.: "Distribution of connectin(titin),nebulin and α-actinin at myotendinous junctions of chicken pectoralis muscles:....." J.Muscle Res.Cell Motil.14. 511-517 (1993)

[Publications] Toyota,N.: "Expression of troponin C genes during development in the chicken." Int.J.Dev.Biol.(in press). (1994)

[Publications] Fukui,A.: "Isolation and characterization of xenopus follistatin and activins." Developmental Biology. 159. 131-139 (1993)

大日方昂千葉大学, 理学部, 教授 (40012413)