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1992 Fiscal Year Final Research Report Summary

CONTROL OF FEEDING AND SEX BEHAVIOR AND DISEASES BY MODULATING HYPOTHALAMIC FUNCTION

Research Project

Project/Area Number 03304024
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

TAKAHASHI Michio  THE UNIVERSITY OF TOKYO FACULTY OF AGRICULTURE. PROFESSOR, 農学部, 教授 (30011943)

Co-Investigator(Kenkyū-buntansha) TATSUYAMA Shigemi  THE UNIVERSITY OF TOKYO FACULTY OF AGRICULTURE, ASSISTANT PROFESSOR, 農学部, 助手 (80219526)
MORI Yuji  THE UNIVERSITY OF TOKYO FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (40157871)
NISHIHARA Masugi  THE UNIVERSITY OF TOKYO FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (90145673)
KURAKAMI Noboru  MIYAZAKI UNIVERSITY FACULTY OF AGRICULTURE, ASSISTANT PROFESSOR, 農学部, 助手 (80150192)
Project Period (FY) 1991 – 1992
KeywordsHYPOTHALAMUS / VENTROMEDIAL NUCLEUS / FEEDING BEHAVIOR / SEX BEHAVIOR / GONADOTROPIN / EXCITATORY AMINO ACIDS / SYMPATHETIC NERVOUS SYSTEM / TUMOR NECROSIS FACTOR
Research Abstract

In this study, we examined neuronal machanisms in the hypothalamus controlling feeding behavior and reproductive function by means of multiunit activity recording technique, water-absorbent polymer injection into the brain, and neuronal cell culture. We found that endogenous excitatory amino acids acting on the Kainate-type receptor in the ventromedial nucleus of the hypothalamus (VMH) facilitated both running activity and the sympathetic nervous system, and that the release of such amino acids from the nerve terminal was presynaptically inhibited by GRBA. The VMH may issue commands to supply energy substrates during the hyper-running and thus integrate both exercise and energy metabolism at the same time. We also found that the electrical activity of the hypothalamic gonadotropin releasing hormone (GnRH) Pulse generator was facilitated by norepinephrine and inhibited by opioid peptides. Further, GnRH Pulse generator activity was found to be suppressed by tumor necrosis factor, a cytokine released from macrophages, via a prostaglandin mediated mechanism. It was suggested that preovulatory gonadotropin surge was induced by a neuronal mechanism that is intrinsically different from the GnRH pulse generator. We established a dissociated rat suprachiasmatic nucleus cell culture which provides a useful model for the analysis of a mammalian circadian system at the cellular level. We identified the 64-KDa protein which may be a part of the circadian clock mechanism. Through these studies, we will be able to resolve problems concerning metabolism and reproduction in the field of veterinary and medical sciences.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Narita,K.,Yokawa.T.,Nishihara,M.,Takahashi,M.: "Interaction between excitatory and inhibitory amino acids in the ventromedial nucleus of the hypothalamus in inducing hyper-running" Brain Research. 603. 243-247 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiruma,H.,Nishihara,M.,Kimura,F.: "Hypothalamic electrical activity that relates to the pulsatile release of luteinizing hormone exhibits diumal variation in ovariectomized rats" Brain Research. 582. 119-122 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 西原 真杉,比留間 弘美,森 裕司: "視床下部電気活動のモニター法ーGnRH分泌にともなう神経活動の変化ー" 日本内分泌学会雑誌. 68. 1-10 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishihara,M.,Hiruma,H.,Kimura,F.: "Interaction between the noradrenergic and opioid peptidergic systems in controlling the electrical activity of luteinizing hormone-releasing hormone pulse generator in ovariectomized rats" Neuroendocrinology. 54. 321-326 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura,F.,Nishihara,M.,Hiruma,H.,Funabashi,T.: "Naloxone Increases the frequency of the electrical activity of luteinzing hormone-releasing hormone pulse generator in long-term ovariectomized rats." Neuroendocrinology. 53. 97-102 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murakani,N.,Takamure,M.,Takahashi,K.,Utunimiya,K.,Kuroda,H.,Etoh,T.: "Ling-term cultured neurons from rat suprachiasmatic nucleus retain the activity for circadian oscillation of vasopressin release" Brain Research. 545. 347-350 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishihara,M.,Hiruma,H.,Kimura,F.: "Brain Control of the Reproductive System" Japan Scientific Societies Press, 193 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishihara,M.,Mori,Y.,Yoo,M.-J.,Takahashi,M.: "Pulsatility in Neuroendocrine Systems" Academic Press, (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Marita K, Yokawa T, Nishihara M and Takahashi M: "Interaction between excitatory and inhibitory amino acids in the ventromedial nucleus of the hypothalamus in inducing hyper-running." Brain Research. 603. 243-247 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiruma H, Nishihara M and Kimura F: "Hypothalamic electrical activity that relates to the pulsatile release of luteinizing hormone exhibits diurnal variation in ovariectomized rats." Brain Research. 582. 119-122 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishihara M, Hiruma H, Mori Y: "Monitoring of hypothalamic electrical activity." Folia Endocrinologica Japonica. 68. 1-10 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishihara M,Hiruma H and Kimura F: "Interaction between the noradrenergic and opioid peptidergic systems in controlling the electrical activity of luteinizing hormone-releasing hormone pulse generator in ovariectomized rats." Neuroendocrinology. 54. 321-326 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura F, Nishihara M, Hiruma H and Funabashi T: "Naloxone Increases the frequency of the electrical activity of luteinizing hormone-releasing hormone pulse generator in long-term ovariectomized rats." Neuroendocrinology. 53. 97-102 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murakami N, Takamure M, Takahashi K, Utunomiya K, Kuroda H and Etoh T: "Long-term cultured neurones from rat suprachiasmatic nucleus retain the capacity for circadian oscillation of vasopressin release." Brain Research. 545. 347-350 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishihara M, Hiruma H and Kimura F: "Generating mechanism of pulsatile gonadotropin release. In: Yokoyama A(ed)Brain Control of the Reproductive System." Japan Scientific Societies Press,Tokyo/CRC Press,Boca Raton. 69-91 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishihara N, Mori Y, Yoo M-J and Takahashi M: "In vivo electrophysiological monitoring of GnRH pulse generator activity in rats and goats." In: Levine JE and Conn MP(eds) Pulsatility in Neuroendocrine Systems. Academic Press,Orland. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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