Survey on scrapie in Japanese : detection of PrP^<Sc> and studies on the distribution of the PrP genotypes in sheep.

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 03304025
• Research Category: Grant-in-Aid for Co-operative Research (A)
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: SHINAGAWA Morikazu Obihiro Univ., Vet Med., Prof., 畜産学部, 教授 (00001537)
• Co-Investigator(Kenkyū-buntansha): HIRAI Katsuya Gifu Univ.Agricult., Prof., 農学部, 教授 (30021702) ; HONDA Eiichi Tokyo Univ.of Agricult. and Tech., Agricult., Ass.Prof., 農学部, 助教授 (20109507) ; MIKAMI Takeshi Tokyo Univ.Agricult., Prof., 農学部, 教授 (20091506) ; ONUMA Misao Hokkaido Univ.Vet.Med., Prof., 獣医学部, 教授 (70109510) ; HORIUCHI Motohiro Obihiro Univ., Vet Med., Instructor., 畜産学部, 助手 (30219216)
• Project Period (FY): 1991 – 1993
• Keywords: SCRAPIE / SURVEY / PrP^<Sc> / RFLP TYPES

Research Abstract

1. Restriction fragment length polymorphism (RFLP) on the sheep-PrP gene : To investigate the RFLP types on the PrP-gene, chromosomal DNAs from sheep tissues collected from various districts in Japan were analyzed using restriction endonucleases EcoRI and HindIII and the coding region of sheep PrP as a probe. Sheep were classified into 6 types. Types I to III corresponded to the types reported in England. Nearly half of the scrapie sheep belonged to type I and the frequency of type II in scrapie sheep was low. Distribution of the types in different parts of Japan showed some variations.
2. Survey of scrapie infected sheep in Japan : To survey the incidence of scrapie infection in Japan, detection of PrP^<Sc> in a total of 197 sheep brains, spleens and lymph nodes obtained from various districts in Japan was carried out. PrP^<Sc> was detected in 16 sheep collected in Hokkaido and in 2 obtained from other districts. However, these two sheep had also been produced in Hokkaido. These findings suggest that contamination with scrapie in Japan is restricted to sheep produced in Hokkaido.

Research Products

• [Publications] Muramatsu,Y.et al.: "A specific RFLP type associated with the occurrence of sheep scrapie in Japan." Arch.Virol.127. 1-9 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shinagawa,M.et al.: "Familial scrapie cases with shorted incubation periods." J.Vet.Med.Sci.55. 665-667 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Muramatsu,y.et al.: "Detection of PrP^<Sc> in sheep at the preclinical stage of scrapie and its significance for diagnosis of insidious infection." Arch.Virol.(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Onodera,A.et al.: "Survey of natural scrapie in Japan:analysis of RFLP types of the PrP gene and detection of PrP^<Sc> mainly in Suffolk sheep." J.Vet.Med.Sci.56(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Muramatsu, Y., et al.: "A specific RFLP type associated with the occurrence of sheep scrapie in Japan" Arch.Virol.127. 1-9 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shinagawa, M., et al.: "Familial scrapie cases with shorted incubation periods." J.Vet.Med.Sci.55. 665-667 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Muramatsu, Y., et al.: "detection of PrP^<Sc> in sheep at the preclinical stage of scrapie and its significance for diagnosis of insidiou infection." Arch.Virol.1994, (in press.).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Onodera, A., et al.: "Survey of natural scrapie in Japan : analysis of RFLP types of the PrP gene and detection of PrP^<Sc> mainly in Suffolk sheep." J.Vet.Med.Sci.568, (in press). (1994)
  • Description: 「研究成果報告書概要(欧文)」より