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1992 Fiscal Year Final Research Report Summary

Immunohistochemical Analysis of Odontogenic and Osteogenic Tumors

Research Project

Project/Area Number 03304041
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionOkayama University

Principal Investigator

NAGAI Noriyuki  Okayama Univ. Dental Sch., Professor, 歯学部, 教授 (90085770)

Co-Investigator(Kenkyū-buntansha) FUKUYAMA Hiroshi  Kyusyu Dental College Professor, 教授 (60037546)
TAKAGI Thoru  Tokyo Med. & Dent. Univ. Sch. of Dent.,Associate Prof., 歯学部, 講師 (20124696)
KUBOKI Yoshinori  Hokkaido Univ. Sch. of Dent., Professor, 歯学部, 教授 (00014001)
NAGATSUKA Hitoshi  Okayama Univ. Dental Sch., Assistant prof., 歯学部, 助手 (70237535)
TAKESHITA Nobuyoshi  Okayama Univ. Dental Sch., Associate prof., 歯学部, 助教授 (00118275)
Project Period (FY) 1991 – 1992
KeywordsBone matrix protein / Osteonectin / Osteocalcin / Collagen / Immunohistochemistry / Bone / Cementum / Periodonteum
Research Abstract

Odontogenic tumors are thought to be originated from epithelial and /or mesenchymal tissue of tooth germ, because of morphological similarities between these tumors and tooth germs. But the origin and histogenesis of most of the odontogenic tumors have been remained to be unproven. Amelogenin, an organic matrix of enamel in the early developmental stage, is well known to be proline rich glycoprotein with low molecular weight, distinct from keratins or collagen molecules(10-11). Since complete amino acid sequence of amelogenin was accomplished by Takagi et al (12) and Yeh et al (13), non cross-reacting monoclonal antibodies against amelogenin were developed. With these monoclonal antibodies, amelogenin was detected ultrastructurally, and merocrine secretion of amelogenin by late preameloblasts and secretory ameloblasts was elucidates. The detection of amelogenin was considered to be useful as a marker of functioning ameloblasts in clarifying the histogenesis of odontogenic tumors.
Immuno … More histochemical expressions of cytokeratin, amelogenin, and enamelin were studies in odontogenic tumors; ameloblastoma, adenomatoid odontogenic tumor, ameloblastic carcinoma. Four anti-cytokeratin monoclonal antibodies were used in this study: CK-1 to total keratin, SE-K to 56,56.5,58 and 68Kd,NSE-K to 52.5Kd,and 19K to 40Kd. Cytokeratin expression in follicular ameloblastoma was similar to that in tooth germ epithelia, and in plexiform ameloblastoma similar to that in fetal oral mucosa. Both types of ameloblastoma also showed slight immunoreactivity for amelogenin. In adenomatoid odontogenic tumor there was a difference between cytokeratin expression in central cells and in peripheral cells in the tumor nests. Moreover, cytokeratin expression in adenomatoid odontogenic tumor was completely different from those in ameloblastoma and tooth germ. Immunoreactivity for amelogenin and enamelin in adenomatoid odontogenic tumor was found in colloidal drops, calcified masses and lining cell membrane of ductlike structure. Therefore, it is possible that these tumor cells differentiate into enamel protein-producing cells, more than those of ameloblastoma do. Immunohistochemical results in ameloblastic carcinoma showed that this carcinoma is composed of undifferentiated odontogenic tumor cells. Also, an immunohistochemical study of localization of type I,II and III collagen were discussed in mandibular condylar cartilage compared with fibrillar bone. Bone matrix protein detected in osteogenic tumors. Less

  • Research Products

    (56 results)

All Other

All Publications (56 results)

  • [Publications] 長塚 仁: "エナメル上皮腫の免疫組織化学的、電子顕微鏡的研究…特に、サイトケラチンと中間径フィラメントについて…" 岡山歯学会雑誌. 10(1). 37-52 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永井 教之: "歯原上皮性腫瘍の細胞骨格、アメロジェニン・エナメリンの局在に関する免疫組織化学的検討" 日本口腔科学会雑誌. 40(4). 746-752 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Nagai: "Ameloblastic carcinoma:case report and review" J Oral Pathology & Medicine. 20(9). 294-297 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 赤木 巧: "ラット顎骨および皮下組織埋入合成水酸化アパタイト表面に出現する多核巨細胞の酵素組織化学的研究" 歯科基礎医学会雑志. 33(3). 275-280 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] N.Takeshita: "Histological and histometric observation of rat jaw bone after implantation of synthetic hydroxyaptite" Jpn J Oral Biol.33(3). 320-322 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新谷 悟: "原始性嚢胞および歯原性角化嚢胞の臨床的、病理組織学的ならびに免疫組織化学的検討" 日本口腔外科学会雑誌. 37(9). 1591-1599 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] 藤井 宰: "低温度焼成水酸化アパタイトコーティング水酸化アパタイトに対する骨形成反応について" 日本口腔インプラント学会雑誌. 5(2). 12-18 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] L.Missana: "Formation of acellular cementum at interface between zirconia ceramic and enamel aspect of rat incisor periodontium" J Jpn Society of Oral Implantology. 4(2). 176-182 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石田 治: "ヒト胎児歯原性上皮のケラチンフィラメントに関する免疫組織化学的、電子顕微鏡的研究" 日本口腔科学会雑誌. 40(1). 19-29 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 永井 教之: "骨・硬組織の標本作製法と免疫組織化学" 検査と技術. 20(2). 135-141 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 日名 雅彦: "ラット歯胚におけるアメロジェニンの免疫組織学化ー脱灰液と酵素処理による影響ー" 硬組織研究技術談話会会誌. 1(1). 21-22 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 長塚 仁: "歯原性腫瘍のアメロジェニン局在に関する免疫組織化学的検討" 硬組織研究技術談話会会誌. 1(1). 23-24 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 石割 裕三: "軟骨・骨のコラーゲン分子種の免疫組織化学的染色における酵素処理の影響" 硬組織研究技術談話会会誌. 1(1). 43-44 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 永井 教之: "BMP各種支持体による異所性骨形成の病理組織学と免疫組織化学" 硬組織研究技術談話会会誌. 1(1). 57-58 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 寒河江 登志朗: "硬組織の結晶成分の電子回析法ならびにX線回析法による分析の長所短所" 硬組織研究技術談話会会誌. 1(1). 11-12 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 永井 教之: "インプラント-骨界面の病理組織学的課題" 歯科ジャーナル. 35(4). 409-416 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori NAGAI: "Histopathological and ultrastuctural studies of oral mucosa with candida infection" Journal of Oral Pathology & Medicine. 21(4). 171-175 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuyoshi TAKESHITA: "Osteoclasic features of multinucleated giant cells responding to the synthetic hydroxyapatite." Journal of electron microscopy. 41(3). 141-146 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山崎 正子: "1回法歯科骨内インプラント(バイオセラム)における初期安静植立術式の有効性に関するX線的観察" 日本口腔インプラント学会誌. 5(2). 19-23 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 永井 教之: "異物に対する原則的生体反応、インプラントに対する口腔粘膜と顎骨の反応:口腔インプラント学(上巻)" 歯科薬出版, 440 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] 永井 教之: "骨・歯牙組織の病理検査法と研究技術の実際" 学際企画, 218 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] Noriyuki NAGAI: "Electron microscopic studies of early calcification on synthetic hydroxyapatite in vitro and in vivo" Japanese Association of Apatite Sciene, 536 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noriyuki NAGAI: "Histological studies of heterotopic osteogenesis induced by bone morphogenetic protein binding hydroxyapatite" Japanese Association of Apatite Sciene, 536 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori KUBOKI: "Hydroxyapatite as a supporter for osteoblast differentiation in vivo and in vitro" Japanese Association of Apatite Sciene, 536 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori KUBOKI: "Osteogenesis induced by BMP-coated biomaterials:Biochemical principles of bone reconstruction in denistry :The bone-biomater interface" University of Toronto Press, 502 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Davies,J.E.: "Deposition of cement-like matrix on implant materilas:The bone-biomaterial interface" University of Toronto Press, 502 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永井 教之: "循環障害、3章 :歯学生のための病理学 一般病理編" 医歯薬出版, 262 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] 永井 教之: "歯科医師のための歯科骨内インプラント入門「1回法・2回法の理論と概要」" 学際企画, 683 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hitoshi NAGATSUKA: "Cytokeratin filaments in ameloblastoma" The Journal of Okayama Dental Society. 10(1). 37-53 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: "Immunohistochemical study of cytokeratin,Amelogenin and enamelin in odontogenic epithelial tumors" J.Jpn.Stomatol. Soc.40(4). 746-752 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: "Ameloblastic carcinoma : case report and review" Journal of Oral Pathology & Medicine. 20(9). 294-297 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takumi AKAGI: "Enzyme histochemical study of multinucleated giant cells responding to synthetic hydroxyapatite in rat jaw bone and subcutaneous tissue" Japanese Journal of Oral Biology. 33(3). 275-280 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyoshi TAKESHITA: "Histological and histometric observation of rat jaw bone after implantation of synthetic hydroxyapatite" Japanese Journal of Oral Biology. 33(3). 320-322 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoru SHINTANI: "A clinical, pathological and immunohistochemical study of primordial cyst and odontogenic keratocyst" Japanese Journal of Oral and Maxillofacial Surgery. 37(9). 1591-1599 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Liliana Missana: "Formation of acellular cementum at interface between zirconia ceramic and enamel aspect of rat incisor periodontium" Journal of Japanese Society of Oral Implantology. 4(2). 176-182 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osamu ISHIDA: "Cytokeratin filaments in odontogenic epithelium of human fetus" J.Jpn Stomatol.Soc.41(1). 19-29 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyoshi TAKESHITA: "Osteoclasic features of multinucleated giant cells responding to the synthetic hydroxyapatite." Journal of Electron microscopy. 41(3). 141-146 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masako YAMAZAKI: "Radiographic study on validity of the rest implantation period for one-step dental implant" Journal of Japanese Society of Oral implantology. 5(2). 12-18 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osamu FUJII: "Behavior of new bone formation of synthetic hydroxyapatite coated with synthetic hydroxyapatite sintered at lowtemperature" Journal of Japanese Society of Oral implantology. 5(2). 12-18 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: "Immunohistochemical technology of hard tissue" Modern Medical Laboratory. 135-141 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mosahiko HINA: "Immunohistochemistry of amelogenin in rat tooth germ - Effects of decalcification and enzyme treatment--" Journal of hard tissue research and technology. 1(1). 21-22 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hitoshi NAGATSUKA: "Immunohistochemical expression of amelogenin in odontogenic tumors" Journal of hard tissue research and technology. 1(1). 23-24 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuzho ISHIWARI: "Effects of enzyme treatment for collagen immunohistochemistry of cartilage and bone" Journal of hard tissue research and technology. 1(1). 43-44 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: "Histopathological studies of new bone formation induced by BMP-carrier composites" Journal of hard tissue research and technology. 1(1). 57-58 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiro SAKAE: "Advantage/disadvantage of diffraction by x-ray and electron beam in study of hard tissue crystals" Journal of hard tissue research and technology. 1(1). 11-12 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: "Histopathological aspect of interface on bone-dental implant" The Journal of Dental Medicine. 35(4). 409-416 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshinori NAGAI: "Histopathological and ultrastructural studies of oral mucosa with candida infection" Journal of Oral Pathology & Medicine. 21(4). 171-175 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: Ishiyaku pub.Oral Implantology Vol.1, 440 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: Gakusai Kikaku. Research technology of hard tissue, 218 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshinori KUBOKI: University of Toronto Press. Osteogenesis induced by BMP-coated biomaterials: Biochemical principles of bone reconstruction in dentistry: The bone -biomaterial interface, 502 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Davies,J.E.: University of Toronto Press. Deposition of cement-like matrix on implant materilas : The bone -biomaterial interface, 502 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: Ishiyaku Pub.General pathology in dental students, 262 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: Gakusai Kikaku. Principles of dental implantology, 683 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: Japanese Association of Apatite Science. Electron microscopic studies of early calcification on synthetic hydroxyapatite in vitro and in vivo., 536 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyuki NAGAI: Japanese Association of Apatite Science. Histological studies of heterotopic osteogenesis induced by bone morphogenetic protein binding hydroxyapatite, 536 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshinori KUBOKI: Japanese Association of Apatite Science. Hydroxyapatite as a supporter for osteoblast differentiation in vivo and in vitro, 536 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24   Modified: 2018-02-02  

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