1991 Fiscal Year Annual Research Report

オルガネラ構成因子の薬物刺激への協調的応答

Research Project

Project/Area Number	03304045
Research Institution	Kanazawa University
Principal Investigator	大場義樹金沢大学, 薬学部, 教授 (10012634)
Co-Investigator(Kenkyū-buntansha)	石橋貞彦広島大学, 医学部, 教授 (90012616) 市原明徳島大学, 酵素研究所, 教授 (40035374) 市川厚京都大学, 薬学部, 教授 (10025695) 野村靖幸北海道大学, 薬学部, 教授 (00034041) 名取俊二東京大学, 薬学部, 教授 (50012662)
Keywords	膜結合性Gキナ-ゼ / 細胞膜タンパク質 / 脂質輸送 / m‐RNA構造 / ホルモン受容体
Research Abstract	当初の研究計画に従って3のグル-プを結成し、研究協力を行った。グル-プ1:膜機能に関するもので、石橋は白血球における活性酸素産生機構を細胞膜蛋白:細胞質蛋白の活性複合体から検討、市川は肥満細胞の細胞膜Gタンパク質aサブユニットの局在性と細胞室移行と脱感作との関係を解析、野村はNG108‐15細胞でセロトニン刺激によりGシクラ-ゼの活性化に際し新規リセプタ-の関与を発見、高野ペルオキシゾ-ム膜形成過程を膜蛋白合成と輸送の面から解析した。グル-プ2:細胞オルガネラの諸反応に関するもので、岡本は分泌顆粒中のC末端アミド化酵素を単離しその作用を解析、さらにその遺伝子構造を明にした。市原はプロテオソ-ム複合体を単離しその性状及び形成過程を研究、加藤はリソゾ-ム顆粒内蛋白の合成からリソゾ-ム膜への輸送過程、井上は細胞内コレステロ-ル輸送系に対するアゾ-ル系薬物の使用、赤松はホスファチジルセリン欠損細胞を単離し、それを利用してPSが小胞体からミトコンドリアへ至る輸送系ならびに生合成系を解析した。グル-プ3:細胞核内因子の作用発現に関するもので、五十嵐はポリアミンに依る蛋白合成促進がmRNAのGC含量に依存することを発見、市山はセリン:ピルビン酸トランスアミナ-ゼ遺伝子の染色体上の位置を決定、大場はラット肝中の甲状腺ホルモンのAGP遺伝子に対する作用発現をクロマチンレベルで解析した。以上全班員はその成果を学会、雑誌等に発表、それぞれ満足すべき評価を得た。

Research Products

(11 results)

All Other

All Publications (11 results)

[Publications] Masanori Hiura: "Stimulation of superoxide anion in guinea pig polymorphonuclear leukocytes by hypotonic conditions in combination with protein kinase C activators" Arch.Biochem.Biophys.291. 31-37 (1991)
[Publications] Satoru Takahasi: "Cytosol promotes the guanine nucleotide‐induced release of the α subunit of G_<i2> from the membrane of mouse mastocytoma P‐815 cells" J.Biol.Chem.266. 5367-5370 (1991)
[Publications] Michihisa Tohda: "The slow cyclic GMP increase caused by serotonin in NG108ー15 cells is not inhibited by antagonist of known serotonin receptors:possible existence of new receptor subtype coupled with membrane‐bound guanylate cyclase" J.Neurochem.57. 714-717 (1991)
[Publications] Osamu Kuge: "A Cloned gene encoding phosphatidylserine decarboxylase complements the phosphatidylserine biosynthetic defect of a chinese hamster ovary cell mutant" J.Biol.Chem.266. 6370-6376 (1991)
[Publications] Sadaki Yokota: "Organelle localization of rat liver serine:pyruvate aminotlansferase expressed in transfected(osー1cells)" Biochem.Res. 12. 53-59 (1991)
[Publications] Nobuaki Kawaguti: "Involvement of sarcophaga lectin in the development of imaginal discs of sarcophaga peregrina in an autocrine manner" Develop.Biol.144. 86-93 (1991)
[Publications] Yuji Sato: "Purification and characterization of the α‐tocopherol transfer protein from rat liver" FEBS Lett.288. 41-45 (1991)
[Publications] Tsuneo Imanaka: "A novel 57kDa peroxisomal membrane polypeptide detected by mono clonal antibody(PXM1a/207B)" Biochem.Biophys.Acta. 1062. 264-270 (1991)
[Publications] Noguti M.,: "The sources of oxygen atom in the α‐hydroxyglycine intermediate of the peptidylglycine α‐amidating reaction" Biochem.J.(1992)
[Publications] Hideyo Yasuda: "A point mutation in C‐terminal region of cdc‐2 kinase causes a G2‐phase arrest in a mouse temperature‐sensitive FM3A cell mutant" Cell Struct.Func.16. 105-112 (1991)
[Publications] Keiko Kasiwagi: "Coexistence of the genes for putrescine transport protein and ornithine decarboxylase at 16 min on Escherichia coli chromosome" J.Biol.Chem.226. 20922-20927 (1991)

1991 Fiscal Year Annual Research Report

オルガネラ構成因子の薬物刺激への協調的応答

Principal Investigator

大場 義樹 金沢大学, 薬学部, 教授 (10012634)

Research Products

[Publications] Masanori Hiura: "Stimulation of superoxide anion in guinea pig polymorphonuclear leukocytes by hypotonic conditions in combination with protein kinase C activators" Arch.Biochem.Biophys.291. 31-37 (1991)

[Publications] Satoru Takahasi: "Cytosol promotes the guanine nucleotide‐induced release of the α subunit of G_<i2> from the membrane of mouse mastocytoma P‐815 cells" J.Biol.Chem.266. 5367-5370 (1991)

[Publications] Michihisa Tohda: "The slow cyclic GMP increase caused by serotonin in NG108ー15 cells is not inhibited by antagonist of known serotonin receptors:possible existence of new receptor subtype coupled with membrane‐bound guanylate cyclase" J.Neurochem.57. 714-717 (1991)

[Publications] Osamu Kuge: "A Cloned gene encoding phosphatidylserine decarboxylase complements the phosphatidylserine biosynthetic defect of a chinese hamster ovary cell mutant" J.Biol.Chem.266. 6370-6376 (1991)

[Publications] Sadaki Yokota: "Organelle localization of rat liver serine:pyruvate aminotlansferase expressed in transfected(osー1cells)" Biochem.Res. 12. 53-59 (1991)

[Publications] Nobuaki Kawaguti: "Involvement of sarcophaga lectin in the development of imaginal discs of sarcophaga peregrina in an autocrine manner" Develop.Biol.144. 86-93 (1991)

[Publications] Yuji Sato: "Purification and characterization of the α‐tocopherol transfer protein from rat liver" FEBS Lett.288. 41-45 (1991)

[Publications] Tsuneo Imanaka: "A novel 57kDa peroxisomal membrane polypeptide detected by mono clonal antibody(PXM1a/207B)" Biochem.Biophys.Acta. 1062. 264-270 (1991)

[Publications] Noguti M.,: "The sources of oxygen atom in the α‐hydroxyglycine intermediate of the peptidylglycine α‐amidating reaction" Biochem.J.(1992)

[Publications] Hideyo Yasuda: "A point mutation in C‐terminal region of cdc‐2 kinase causes a G2‐phase arrest in a mouse temperature‐sensitive FM3A cell mutant" Cell Struct.Func.16. 105-112 (1991)

[Publications] Keiko Kasiwagi: "Coexistence of the genes for putrescine transport protein and ornithine decarboxylase at 16 min on Escherichia coli chromosome" J.Biol.Chem.226. 20922-20927 (1991)

大場義樹金沢大学, 薬学部, 教授 (10012634)