| Project/Area Number |
03304050
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Nagoya University School of Medicine (1993)
Kagoshima University (1991-1992) |
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Principal Investigator |
MURAMATSU Takashi Nagoya University School of Medicine, Dept.Biochemistry, Professor, 医学部, 教授 (00030891)
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| Co-Investigator(Kenkyū-buntansha) |
IRIMURA Tatsuro University of Tokyo, Faculty of Pharmaceutical Science, Professor, 薬学部, 教授 (80092146)
KAWASAKI Toshisuke Kyoto University Faculty of Pharmaceutical Science, Dept.Biochemistry, Professor, 薬学部, 教授 (50025706)
ENDO Masahiko Hirosaki University School of Medicine, Dept.Biochemistry, Professor, 医学部, 教授 (20004616)
NAGAI Yoshitaka Tokyo Metropolitan Institute of Medical Science, Director, 所長 (80072974)
SAITO Masaki Jichi Medical School, School of Medicine, Institute of Blood Medicine, Professor, 医学部, 教授 (60012762)
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| Project Period (FY) |
1991 – 1993
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| Keywords | Glycoprotein / Glycolipid / Ganglioside / Cell adhesion / Glycosyltransferase / Lectin / Mucin / Carbohydrate deficience |
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| Research Abstract |
Transfection and forced expression of alpha-1, 3-fucosyltransferase resulted in increased cell-substratum adhesion. Glycosyltransferases regulating glycolipid biosyntesis in human blood cells were identified. Structure of glycans bearing Le^a antigenic epitope was elucidated in the human colon. Regional difference was found in sulfation paltern of mucin carbohydrates isolated from the human stomach. Protein structure was determined for beta-1, 6-N-acetylglucosamine transferase, which is involved in the synthesis of Gsl-5. Mutation leading to Lewis blood group-negative blood type was elucidated at the DNA sequence level. A new hybrid glycosaminoglycan was produced by utilizing transglycosylation activity of hyaluronidase. Tyrosine residue(try-5) of macrophage lection was required for internalization of the lectin. Conagglutinin, a C-type lectin, was cDNA cloned and the genome structure was elucidated. Protein-carbohydrate linkage region of chondroitin sulfate was synthesized by organic synthesis.
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