1993 Fiscal Year Annual Research Report

細胞運動におけるMotor蛋白質の役割

Research Project

Project/Area Number	03304054
Research Institution	Gunma University School of Medicine
Principal Investigator	小浜一弘群馬大学, 医学部, 教授 (30101116)
Co-Investigator(Kenkyū-buntansha)	大室弘美東京大学, 医学部, 助手 (00124470) 石川良樹群馬大学, 医学部, 助手 (20212863) 黒田英世富山大学, 理学部, 教授 (50064845) 田中利男三重大学, 医学部, 教授 (00135443) 永井玲子大阪大学, 教養部, 教授 (90028192)
Keywords	モーター蛋白質 / アクチン / ミオシン / カルシウム / 制御機能 / モーティリティー・アッセイ / 核磁気共鳴 / 遠心顕微鏡
Research Abstract	筋細胞のみならずあらゆる細胞には顕微鏡下では何らかの可視的な動きがみられる。この細胞運動一般は特殊な場合を除いてATPの化学エネルギーを“うごき"という機械的エネルギーに変換する蛋白質群によって担われている。これらはMotor蛋白質と呼ばれていて、アクチン・ミオシン系はこれに当たる。しかし、Motor蛋白質の試験管内での機能は単純であるため、これがどの様に多様な細胞運動を発現しているかという役割の問題にはほとんど手がけられていない。本研究ではこの役割を明らかにするために(1)新しいMotor蛋白質の検索、(2)アクチン・ミオシン系の機能を制御する新しい蛋白質の発見、(3)近年のアクチン・ミオシン系の研究に取り入れられて実績をあげつつある新しい技術(i,In Vitro Motility Assay;ii,蛋白質一次構造決定;iii,核磁気共鳴;iv,遠心顕微鏡法)の導入を目的としている。 3年間の研究計画の実施により(1)はいくつかの植物細胞よりミオシン様蛋白質の発見に、(2)は平滑筋細胞のミオシン軽鎖キナーゼの新しい機能の発見に結びつけることができた。(3)ではi)を平滑筋収縮制御の研究に、ii)を先体反応研究に応用することができた。さらにiii)はミオシンのmotor機能分析に、iv)はアクチンのmotor機能分析に応用することが出来た。

Research Products
(14 results)

All Other

All Publications (14 results)

[Publications] Masuda,T.,et al.: "Confocal laser microscopy of dystrophin localization in guinea pig skeletal muscle fibers." J.Cell Biol.119. 543-548 (1992)
[Publications] Ishikawa,R.,et al.: "Stimulation of the interaction between actin and myosin by Physarum caldesmon-like protein and smooth muscle caldesmon." J.Biol.Chem.266. 21784-21790 (1991)
[Publications] Kamitsubo,E.,et al.: "Immobilization of endoplasm flowing contiguous to the actin cables upon electrical stimulus in Nitella internodes." Protoplasma. 168. 82-86 (1992)
[Publications] Kobayashi,H.,et al.: "Isolation of cDNA for 155kDa protein exhibiting myosin light chain kinase activity." J.Biochem.112. 786-791 (1992)
[Publications] Kohno,T.,et al.: "Partial purfication of myosin from lily pollen tubes by monitoring with in vitro motility assay." Protplasma. 170. 77-85 (1992)
[Publications] Takagi,T.,et al.: "Structure of 29 kDa protein from ascidian(Halocynthia roretzi)body wall muscle." J.Biochem.113. 321-326 (199)
[Publications] Kohama,K.,et al.: "A novel regulatory effect of myosin light chain kinase from smooth muscle on the ATP-dependent interaction between actin and myosin." Biochem.Biophys.Res.Commun.184. 1204-1211 (1992)
[Publications] Nakamura,F.,et al.: "Identification of the regulatory site in smooth muscle calponin that is phosphorylated by protein kinase C." J.Biol.Chem.268. 6194-6201 (1993)
[Publications] Tanokura,M.and Ebashi,S.: "Complexes of myosin subfragment 1 with pyrophosphate and with adenosine diphosphate as studied by phosphorus- 1 nuclear magnetic resonance." J.Biochem.113. 19-21 (1993)
[Publications] Masuda,Y.,et al.: "Protease-sensitive anchoring of microfilament bundles provides tracks for cytoplasmic streaming in Vallisneria." Protoplasma. 162. 151-159 (1991)
[Publications] Furuhashi,K.,et al.: "Phoshporylation by actin kinase of pointed end-domain on the actin molecule." J.Biol.Chem.297. 9326-9330 (1992)
[Publications] Higashi-Fujime,S.: "Reconstitution of active movement in vitro based on the actin-myosin interaction." Int.Rev.Cytl.125. 95-138 (1991)
[Publications] Kazuhiro Kohama: "Calcium Inhibition" Japan Sci.Soc.Press,and CRC Press., 205 (1992)
[Publications] Kazuhiro Kohama,Ryoki Ishikawa,and Tsuyoshi Okagaki: "Calcium Inhibition of Physarum Actomyosin System:Myosin-linked and Actin-linked Natures.In Calcium Inhibition p.p.91-107." Japan Sci.Soc.Press,and CRC Press.K.Kohama,ed., 16 (1992)

1993 Fiscal Year Annual Research Report

細胞運動におけるMotor蛋白質の役割

Principal Investigator

小浜 一弘 群馬大学, 医学部, 教授 (30101116)

Research Products

[Publications] Masuda,T.,et al.: "Confocal laser microscopy of dystrophin localization in guinea pig skeletal muscle fibers." J.Cell Biol.119. 543-548 (1992)

[Publications] Ishikawa,R.,et al.: "Stimulation of the interaction between actin and myosin by Physarum caldesmon-like protein and smooth muscle caldesmon." J.Biol.Chem.266. 21784-21790 (1991)

[Publications] Kamitsubo,E.,et al.: "Immobilization of endoplasm flowing contiguous to the actin cables upon electrical stimulus in Nitella internodes." Protoplasma. 168. 82-86 (1992)

[Publications] Kobayashi,H.,et al.: "Isolation of cDNA for 155kDa protein exhibiting myosin light chain kinase activity." J.Biochem.112. 786-791 (1992)

[Publications] Kohno,T.,et al.: "Partial purfication of myosin from lily pollen tubes by monitoring with in vitro motility assay." Protplasma. 170. 77-85 (1992)

[Publications] Takagi,T.,et al.: "Structure of 29 kDa protein from ascidian(Halocynthia roretzi)body wall muscle." J.Biochem.113. 321-326 (199)

[Publications] Kohama,K.,et al.: "A novel regulatory effect of myosin light chain kinase from smooth muscle on the ATP-dependent interaction between actin and myosin." Biochem.Biophys.Res.Commun.184. 1204-1211 (1992)

[Publications] Nakamura,F.,et al.: "Identification of the regulatory site in smooth muscle calponin that is phosphorylated by protein kinase C." J.Biol.Chem.268. 6194-6201 (1993)

[Publications] Tanokura,M.and Ebashi,S.: "Complexes of myosin subfragment 1 with pyrophosphate and with adenosine diphosphate as studied by phosphorus- 1 nuclear magnetic resonance." J.Biochem.113. 19-21 (1993)

[Publications] Masuda,Y.,et al.: "Protease-sensitive anchoring of microfilament bundles provides tracks for cytoplasmic streaming in Vallisneria." Protoplasma. 162. 151-159 (1991)

[Publications] Furuhashi,K.,et al.: "Phoshporylation by actin kinase of pointed end-domain on the actin molecule." J.Biol.Chem.297. 9326-9330 (1992)

[Publications] Higashi-Fujime,S.: "Reconstitution of active movement in vitro based on the actin-myosin interaction." Int.Rev.Cytl.125. 95-138 (1991)

[Publications] Kazuhiro Kohama: "Calcium Inhibition" Japan Sci.Soc.Press,and CRC Press., 205 (1992)

[Publications] Kazuhiro Kohama,Ryoki Ishikawa,and Tsuyoshi Okagaki: "Calcium Inhibition of Physarum Actomyosin System:Myosin-linked and Actin-linked Natures.In Calcium Inhibition p.p.91-107." Japan Sci.Soc.Press,and CRC Press.K.Kohama,ed., 16 (1992)

小浜一弘群馬大学, 医学部, 教授 (30101116)