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1993 Fiscal Year Final Research Report Summary

Folding and crystallization conditions of menbrane protein supercomplexes

Research Project

Project/Area Number 03304056
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionHimeji Institute of Technology

Principal Investigator

YOSHIKAWA Shinya  Himeji Inst.of Tech.Prof., 理学部, 教授 (40068119)

Co-Investigator(Kenkyū-buntansha) MITAKU Seiki  Tokyo Agr.-Tech.Univ.Assoc.Prof., 工学部, 助教授 (10107542)
TOYOSHIMA Chikashi  Tokyo Inst.of Tech.Assoc.Prof., 生命理工学部, 助教授 (70172210)
TSUKIHARA Tomitake  Tokushima Univ.Prof., 工学部, 教授 (00032277)
SHIRAKIHARA Yasuo  Hyogo Educ.Univ.Assoc.Prof., 自然系物理, 助教授 (20150287)
KOUYAMA Tsutomu  Riken Researcher, 先任研究員 (30170210)
Project Period (FY) 1990 – 1993
KeywordsMembrane protein / Supermolecular complex / X-ray crystallographic analysis / Electron micrographic analysis / Folding mechanism / Crystal growth mechanism / Crystallization / Three dimensional-stractural prediction
Research Abstract

This research project has provided the following conclusion about the folding mechanism and the crystallization method of membrane protein. Three dimensional structure of the membrane protein supercomplexe spanned across the biological membrane in the cell is stabilized by hydrophilic loops exposed to aquaous medium as well as by alpha-helices placed under the ydrophobic environment in the membrane which provide hydrogen bonds and electrostatic interactions between these helices. Thus, solubilization with a detergent which covers the hydrophobic surface seems the best method for isolation of this type of protein complex without denaturation. The specific interaction between the protein melocules which stabilizes the crystalline state is likely to be the one between the hydrophilic surfaces, suggesting that larger homologous membrane protein is better for crystallization than the smaller one. This conclusion is suppoted by the fact that only the biggest cytochrome c oxidase and cytochrome bc1 complex, isolated from beef heart mitochondria, among many homologous proteins have been crystallized. Structure of the detergent molecules which attach to the hydrophobic surfaces of membrane proteins could control the specific interactions between the hydrophilic surface of the protein moleules to determine the crystallization conditions. The control mechanism is not so simple as has been proposed by Michel et al.

  • Research Products

    (122 results)

All Other

All Publications (122 results)

  • [Publications] T.Kobayashi,M.Terauchi,T.Kouyama,M.Yoshizawa,M.Taiji.: "Femtosecond spectroscopy of acidified and neutral bacteriorhodopsin." Soc.Photo-Optic.Instr.Engineer.1403. 407-416 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Fukuda,T.Kouyama.: "Photoreaction of bacteriorhodopsin at high pH:Origins of the slow decay components of M." Biochemistry. 31. 11740-11747 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Fukuda,T.Kouyama.: "Foramtion and decay kinetics of bacteriorhodopsin's N intermediate:Softening the protein conformation with alcohols affects intraprotein proton transfer and retinal isomerization." Photochem.Photobiol.56. 1057-1062 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kouyama.: "Bacteriorhodopsin,a bilogical proton pump working in the light." RIKEN Review. 1. 5-6 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Uehara,K.Kawai,T.Kouyama.: "Photoelectric response of oriented purple membrane electrodeposited onto poly(vinyl alcohol)film" Thin Solid Films,. 232. 271-277 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kouyama,M.Yamamoto,N.Kamiya,H.Iwasaki,T.Ueki,I.Sakurai.: "Polyhedral assembly of a membrane protein in its three-dimensional crystal." J.Mol.Biol.236(in the press.). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 神山 勉: "光駆動プロトンポンプ:生体膜における物質輸送についての考察" 日本の科学と技術. 32巻262号. 61-65 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 神山 勉: "膜蛋白質の超高次構造:バクテリオロドプシンの結晶中に見いだされた巨大な球殻構造(印刷中)" 蛋白質・核酸・酵素.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 月原冨武: "超分子複合体の構造決定 どこまで大きな分子に迫まれるか?" 生物物理. 33. 24-40 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 月原冨武ほか: "超分子複合隊のX線結晶構造解析" 蛋白質核酸酵素. 38. 1247-1253 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 神谷信夫: "X線結晶学とシンクロトロン放射光--新しい光への大きな期待--" 生物物理. 33. 36-37 (1993)

    • Description
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  • [Publications] Shirakihara,Y.Yohda,M.,Kagawa Y.,Yokoyama K.,and Yoshida,M.: "Purification by Dye-Ligand Chromatography and a Crystallization Study of The F1-ATP ase and Its Major Subunits,β and α,from a Ther mophilic Bacterium PS3." J.Biochem. 109. 466-471 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suwa,M.,Mitaku,S.Shimazaki K.and Chuman T: "Characterization of Transmembrane Helices by a Probe Helix of Molecular Energy Calculation." Jpn.J.Appl.Phys.31. 951-956 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suwa,M.,Mitaku,S.Shimazaki K.and Chuman T: "A computer system for predicting membrane protein structure." Computer Aided Innovation of New Materials II.1331-1334 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kubota et al.: "Crystallization and preliminary X-ray crystallographic studies of bovine heart mitochondrial cytochrome bc_1 complex" Journal of Molecular Biology. 221. 379-382 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Yoshikawa et al.: "Strategies for crystallization of large membrane protein complexes" Journal of Crystal Growth. 122. 298-302 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Elgersma,A.V.,安宅光雄、曽良達生: "Kinetic studies on the growth of three crystal forms of lysozyme based on the measurement of protein and Cl^- concentration changes" Journal of Crystal Growth. 122. 31-40 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安宅光雄、新沢恭子、吉川信也: "Phase diagram of a crystalline membrane protein,bovine heart cytochrome c oxidase,in the salting-in region." Journal of Crystal Growth. 122. 60-65 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Vekilov,P.G.,安宅光雄、曽良達生: "Laser Michelson interferometry investigation of protein crystal growth." Journal of Crystal Growth. 130. 317-320 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安宅光雄: "Protein crystal growth:An approach based on phase diagram determination." Phase Transitions. 45. 205-219 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫: "蛋白工学とコンピューター蛋白質のX線結晶構造解析" CICSJ Bulletin. 11(4). 29-32 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫: "生体高分子の単結晶構造解析における中性子回析" 波紋. 3(3). 17-21 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫: "特集「タンパク質結晶学の新世代」新しいターゲットへの挑戦,膜タンパク質へのアプローチ-構造・機能の新天地への挑戦" 生物物理. 33. 324-328 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Miki,et al.: "Application of Automatic Molecular Replacement Procedure to Crystal Structure Analysis of Cytochrome c_2 from Rhodopseudomonas viridis." Acta Crystallogr.D50(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫: "膜タンパク質の構造モティーフ" 化学. 47. 496-497 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫: "タンパク質結晶構造解析総括プログラムシステム,“PROTEIN"の現況" 日本結晶学会誌. 34. 365-366 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 別所康男、安宅光雄、浅井道彦、曽良達生: "Analysis of the crystallization kinetics of lysozyme using a model with polynuclear growth mechanism." Biophysical Journal. 66. 310-313 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫: "分子設計の展望-生体高分子における分子設計" 分子設計. 1. 7-13 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新村信雄、峯崎善章、安宅光雄、曽良達生: "Small angle neutron scattering from lysozyme in unsaturated solutions,to characterize the pre-crystallization process." Journal of Crystal Growth. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小田原孝行、安宅光雄、曽良達生: "Determination of phase diagram to elucidate the crystal growth of Rhodobacter sphaeroides photoreaction center." Acta Crystallographica,Part D.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安宅光雄: "蛋白質結晶化過程の解析" 蛋白質核酸酵素. 37. 529-539 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安宅光雄: "タンパク質の結晶成長" 酵素工学ニュース. No.27. 14-16 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安宅光雄: "蛋白質の結晶成長機構" 日本結晶学会誌. 34. 238-243 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安宅光雄、小川智哉: "Nucleation and growth of oxide precipitates in CZ-Si wafers." Journal of Materials Research. 8. 2889-2892 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫 他: "光合成システムと反応中心のX線構造解析" 太陽エネルギー. 19. 12-18 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫 他: "タンパク質の分類ファミリーは1000種?" 日本結晶学会誌. 35. 48-50 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada,Y.,et al.: "Total Synthesis of a Gene for Octopus Rhodopsin and Its Preliminary Expression、" J.Biochem.110. 501-507 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Deng,H.,et al: "Resonance Raman Studies of the HOOP Mode in Octopus Bathorhodopsin with Deuterium Labeled Retinal Chromophores." Biochemistry,. 30. 4495-44502 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tsuda,et al: "Interaction of Rhodopsin,G-protein and Kinase in Octopus.Photoreceptors." Photochem.Photobiol.56. 1167-1172 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Taiji,et al: "Femtosecond Studies of Primary Photoprocesses in Octopus Rhodopsin." Photochem.Photobiol.56. 1013-1018 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Masuda,et al: "Infrared Studies of Octopus Rhodopsin" FEBS Lett.317. 223-227 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Masuda,et al: "Infrared Studies of Octopus Rhodopsin and Lumi-rhodopsin." J.Mol.Struct.297. 29-34 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama,H.: "Idcntification of the 1,4-Dihydropyridine Binding Regions within the al Subunit of Skclctal Muscle Ca^<2+> Channcls by Photoaffinity Labeling." Proc.Natl.Acad.Sci.USA.88. 9203-9207 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama,H.: "Photolabeled Sites with a Tetrodotoxin Derivative in the Domain III and IV of the Electroplax Sodium Channel." Biochem.Biophys.Res.Commun.184. 900-907 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatanaka,Y.: "Photoaffinity Labeling of the Electroplax Sodium Channel with a Photoreactive μ-Conotoxin Carring a Radioactive and Chromogenic Derivative." Chem Pharm.Bull.40. 2537-2539 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuniyasu,A.: "Structural Characterization of the Cardiac Calcium Channel from Porcine Hearts." J.Biochem.112. 235-242 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama,H.: "Characterization of Saccharide Moiety in the Electroplax Sodium Channel." Chem.Pharm.Bull.40. 3287-3291 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama,H.: "A Site-Directed Antibody Which Inhibits Phosphorylation of the Rat Brain Sodium Channel by cAMP-depemdent Protein Kinase." Biochim.Biophys.Acta. 1175. 67-72 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I.Fujii,et al.: "Evaluation of X-ray Diffraction Data from Protein Crystals with Use of an Imaging Plate." Acta Crystallogr.B47. 137-144 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Kita,et al.: "Crystallization and Preliminary X-ray Diffraction Studies of a Flavoprotein,FP390 from a Luminescent Bacterium,Photobacteriumphosphoreum." J.Biochem.110. 748-750 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.Briozzo,et al.: "Crystallization and Preliminary X-ray Diffraction Studies of C-1027-AG,the Apoprotein of the Macromolecular Antitumor Antibiotic C-1027 from Streptomyces globisporus." Acta Crystallogr.D49. 372-374 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Hayano,et al.: "PDI and Glutathione-mediated Reduction of the Glutathionylated Variant of Human Lysozyme" FEBS Lett.328. 203-208 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Miki,et al.: "Crystallization and Preliminary X-ray Diffraction Studies of Photolyase(Photoreactivating Enzyme)from the Cyanobacterium Anacystis nidulans." J.Mol.Biol.233. 167-169 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Nakane,et al.: "Crystallization and Preliminary X-ray Diffraction Studies of Bacillus stearothermophilus Farnesyl Diphosphate Synthase Expressed in Eschericha coli." J.Mol.Biol.233. 787-788 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama,H.: "A Topographical Study of the Electroplax Sodium Channel with Site-Directed Antibodies" Biochim.Biophys.Acta. 1145. 134-140 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kalasz,H.: "Identification of 1,4-Dihydropyridine Binding Domains within the Primary Structure of the al Subunit of the Skeletal Muscle L-type Calcium Channel." FEBS Letters. 331. 177-181 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe,T.: "Azidobutyryl Clentiazem,A New Photoactivatable Diltiazem Analog,Labels Benzothiazepine Binding Sites of the Calcium Channel." FEBS Letters. 334. 261-264 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Toyoshima: "Three-dimensional cryo-electron microscopy of the calcium ion pump in the sarcoplamisc reticulum membrane." Nature. 362. 469-471 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Toyoshima: "Contrast transfer for the frozen hydrated specimen.II:Amplitude contrast at very low frequencies." Ultramicroscopy. 48. 165-176 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Sato: "Two-dimensional crystallization of catalase on a monolayer film of poly(1-benzyl-L-histidine)spread at the air/water interface." Biochim.Biophys.Acta. 1162. 54-60 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 豊島 近: "膜蛋白質の電子線三次元構造解析" 蛋白質・核酸・酵素. 38. 1276-1286 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 白木原康雄: "膜タンパク質の結晶解析." 日本生化学会編、東京化学同人, 9 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫(分担執筆): "バイオ・高分子研究法5 バイオ・高分子研究における物理化学計測とその応用" 学会出版センター,東京, 256 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫(分担執筆): "化学便覧,基礎編II,改訂4版(日本化学会編)" 丸善,東京, 762 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tsuda,et al: "Photobiology;The Science and its Application," Plenum Press,New York., 1083 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tsuda,et al: "Frontiers of Photobiology" Elsevier Science Publishers., 568 (1993)

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  • [Publications] Nakayama,H.: "Methods in Pharmacology,vol.7(eds.H.Glossmann,J.Striessning)" Plenum Press(New York), 280 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中山 仁: "生体膜工学(Maruzen Advanced Technologyシリーズ,第7巻、二井将光・編)6章“イオンチャンネル構造とイオン輸送機構"" 丸善, 330 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Sogabe,et al.(分担執筆)(Ed.Murata,N.): "Research in Photosynthesis,Vol.II" Kluwer,Dordrecht, 879 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木邦夫(分担執筆): "新生化学実験講座,第5巻:生体酸化・薬物代謝" 東京化学同人,東京, 432 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Elgersma, A.V., Ataka, M.and Katsura, T.: "Kinetic studies on the growth of three crystal forms of lysozyme based on the measurement of protein and Cl^- concentration changes." Journal of Crystal Growth. 122. 31-40 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ataka, M., Shinzawa-Itoh, K.and Yoshikawa, S.: "Phase diagram of a crystalline menbrane protein, bovine heart cytochrome c oxidase, in the salting-in region." Journal of Crystal Growth. 122. 60-65 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Vekilov, P.G., Ataka, M.and Katsura, T.: "Laser Michelson Interferometry investigation of protein crystal growth" Journal of Crystal Growth. 130. 317-320 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ataka, M.: "Protein crystal growth : An approach based on phase diagram determination" Phase Transitions. 45. 205-219 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ataka, M.and Ogawa, T.: "Nucleation and growth of oxide precipitates in CZ-Si wafers." Journal of Materials Research. 8. 2889-2892 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bessho, Y., Ataka, M., Asai, M.and Katsura, T.: "Analysis of the cristallization kinetics of lysozyme using a model with polynuclear growth mechanism." Biophysical Journal. 66. 310-313 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Niimura, N., Minezaki, Y., Ataka, M.and Katsura, T.: "Small angle neutron scattering from lysozime in unsaturated solutions, to characterize the pre-crystallization process." Journal of Crystal Growth. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Odahara, T., Ataka, N.and Katsura, T.: "Determination of phase diagram to elucidate the crystal growth of Rhodobacter sphaeroides photoreaction center." Acta Crystallographica. D.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi, T., Terauchi, M., Kouyama, T., Yoshizawa, M.and Taiji, M.: "Femtosecond spectroscopy of acidified and neutral bacteriorhodopsin." Soc.Photo-Optic.Instr.Engineer.1403. 407-416 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda, K.and Kouyama, T.: "Photoreaction of bacteriorhodopsin at high pH : Origins of the slow decay components of M." Biochemistry. 31. 11740-11747 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda, K.and Kouyama, T.: "Formation and decay kinetics of bacteriorhodopsin's N intermediate : Softening the protein conformation with alcohols affects intraprotein proton transfer and retinal isomerization." Photochem.Photobiol.56. 1057-1062 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kouyama: "Bacteriorhodopsin, a bilogical proton pump working in the light." RIKEN Review. 1. 5-6 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Uehara, K.Kawai and T.Kouyama: "Photoelectric response of oriented purple membrane electrodeposited onto poly(vinyl alcohol) film." Thin Solid Films. 232. 271-277 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kouyama, M.Yamamoto, N.Kamiya, H.Iwasaki, T.Ueki and I.Sakurai: "Polyhedral assembly of a membrane protein in its three-dimensional crystal" J.Mol.Biol. 236 (in the press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kono, J.Y.Wang, I.Sakurai and T.Kouyama: "Precrystallized bacteriorhodopsin polyhedra : native environment in a vesicular structure" Biophys.J.(submitted). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kouyama: "Supramolecular assembly of a membrane protein : Hollow spheres of bacteriorhodopsin in its three dimensional crystal" Protein, Nucleic Acid and Enzyme. 39. 231-240

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama, H., Taki, M., Striessing, J., Glossmann, H., Catterall, W.A., Kanaoka, Y.: "Identification of the 1,4-Dihydrophridine Binding Regions within the alpha 1 Subunit of Skeletal Muscle Ca^<2+> channels by Photoaffinity Labeling with Diazipine" Proc.Natl.Acad.Sci.USA.88. 9203-9207 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama, H., Hatanaka, Y., Yoshida, E., Oka, K., Takanohashi, M., Amano, Y., Kanaoka, Y.: "Photolabeled Sites with a Tetrodotoxin Derivative in the Domain III and IV of the Electroplax Sodium Channel." Biochem.Biophys.Res.Commun.184. 900-907 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatanaka, Y., Nakayama, H., Kanaoka, Y.: "Photoaffinity Labeling of the Electroplax Sodium Channel with a Photoreactive mu-Conotoxin Carring a Radioactive and Chromogenic Derivative." Chem Pharm.Bull.40. 2537-2539 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuniyasu, A., Oka, K., Ide-Yamada, T., Hatanaka, Y., Abe, T., Nakayama, H., Kanaoka, Y.: "Structural Characterization of the Cardiac Calcium Channel from Procine Hearts." J.Biochem.112. 235-242 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama, H., Shikano, H., Kanaoka, Y.: "A Site-Directed Antibody Which Inhibits Phosphorylation of the Rat Brain Sodium Channel by cAMP-depemdent Protein Kinase." Biochim.Biophys.Acta. 1175. 67-72 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama, H., Nakayama, K., Nonomura, Y., Kobayashi, M., Kangawa, K., Matsuo, H., Kanaoka, Y.: "A Topographical Study of the Electroplax Sodium Channel with Site-Directed Antibodies." Biochim.Biophys.Acta. 1145. 134-140 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kalasz, H., Watanabe, T., Yabana, H., Itagaki, K., Naito, K., Nakayama, H., Schwartz, A., Vaghy, P.L.: "Identification of 1,4-Dihydropyridine Binding Domains within the Primary Structure of the alpha1 Sbuunit of the Skeletal Muscle L-type Calcium Channel." FEBS Letters. 331. 177-181 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, T., Kalasz, H., Yabana, H., Kuniyasu, A., Mershon, J., Itagaki, K., Vaghy, P.L., Naito, K., Nakayama, H., Schwartz, A.: "Azidobutyryl Clentiazem, A New Photoactivatable Diltiazem Analog, Labels Benzothiazepine Binding Sites in the al subunit of the Skeletal Muscle Calcium Channel." FEBS Letters. 334. 261-264 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suwa, M., Mitaku, S., Shimazaki K.and Chuman T.: "Characterization of Transmembrane Helices by a Probe Helix of Molecular Energy Calculation." Jpn.J.Appl.Phys.31. 951-956 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suwa, M., Mitaku, S.and Saito, N.: "A computer system for predicting membrane protein sturucture." Computer Aidea Innovation of New Materials. II. 1331-1334 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujii, I., Morimoto, Y., Higuchi, Y., Yasuoka, N., Katayama, C., and Miki, K.: "Evaluation of X-ray Diffraction Data from Protein Crystals with Use of an Imaging Plate." Acta Crystallogr.B47. 137-144 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kita, A., Kasai, N., Kasai, S., Nakaya, T., and Miki, K.: "Crystallization and Preliminary X-ray Diffraction Studies of a Flaboprotein, FP390 from a Luminescent Bacterium, Photobacteriumphosphoreum." J.Biochem.110. 748-750 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Briozzo, P., Inaka, K., Minami, Y., Otani, T., and Miki, K.: "Crystallization and Preliminary X-ray Diffraction Studies of C-1027-AG, the Apoprotein of the Macromolecular Antitumor Antibiotic C-1027 from Streptomyces globisporus." Acta Crystallogr.D49. 372-374 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Deng, H., Manor, D., Weng, G., Rath, P., Koutalos, Y., Ebrey, Gebhard, R., Lugtenburg, J., Tsuda, M.and Callender, H.: "Resonance Raman Studieds of Octopus Bathorhodopsin with Deuterium Lbeled Retinal Chromophores" Photochem.Photobiol.54. 1001-1007 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tsuda, H.Hirata, and T.Tsuda: "Interaction of Rhodopsin, G-protein and Kinase in Octopus. Photoreceptors." Photochem.Photobiol.56. 1167-1172 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Taiji, K.Bryl, M.Nakagawa, M.Tsuda, and Kobayashi: "Femtosecond Studies of Primary Photoprocesses in Octopus Rhodopsin" Photochem.Photobiol.56. 1013-1018 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Masuda, E.H.Morita, M.Tasumi, T.Iwasa and M.Tsuda: "Infrared Studies of Octopus Rhodopsin : Existence of a Long lived Intermediate and the States of the Carboxylic Group of Asp-81 in Rhodopsin and its Photoproducts." FEBS Lett.317. 223-227 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Masuda, E.H.Morita, M.Tasumi, T.Iwasa, M.Tsuda: "Infrared Studies of Octopus Rhodopsin and Lumi-rhodopsin." J.Mol.Struct.297. 29-34 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kikkawa, N.Nakagawa, T.Iwasa, and M.Tsuda: "GTP-binding Proteins Couples with Metabotropic Glutamate Receptor in Bovine Retinal On-bipolar Cell." Biochem.Biophys.Res.Commun.195(1). 347-379 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirakihara, Y., Yoshida, M., Kagawa, Y., Yokoyama K, and Yoshida, M.: "Purification by Dye-Ligand Chromatography and a Crystallization Study of The F1-ATPase and Its Major Subunits, beta and alpha, from a Termophilic Bacterium PS3." J.Biochem. 109. 466-471 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirakihara, Y.: "Crystal Analysis of Membrane Proteins" Shin-Seikagaku-Jikken-Kouza. 66-74 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayano, T., Inaka, K., Otsu, M., Taniyama, Y., Miki, K., Matsushima, M.and Kikuchi, M.: "PDI and Glutathione-mediated Reduction of the Glutathionylated Variant of Human Lysozyme." FEBS Lett.328. 203-208 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miki, K., Tamada, T., Nishida, H., Inaka, K., Yasui, A., de Ruiter, P.E.and Eker, A.P.M.: "Crystallization and Preliminary X-ray Diffraction Studies of Photolyase (Photo-reactivating Enzyme) from the Cyanobacterium Anacystis nidulans." J.Mol.Biol.233. 167-169 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakane, H., Koyama, T., Obata, S., Osabe, M., Takeshita, A., Nishino, T., Ogura, K., and Miki, K.: "Crystallization and Preliminary X-ray Diffraction Studies of Bacillus stearothermophilus Farnesyl Diphosphate Synthase Expressed in Escherichia coli." J.Mol.Biol.233. 787-788 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miki, K., Sogabe, S., Uno, A., Ezoe, T., Kasai, N., Saeda, M., Matsuura, Y.and Miki, M.: "Application of Automatic Molecular Replacement Procedure to Crystal Structure Analysis of Cytochrome C_2 from Rhodopseudomonas viridis." Acta Crystallogr. D50 (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kubota, T., Kawamoto, M., Fukuyama, K., Shinzawa-Itoh, K., Yoshikawa, S., and Matsubara, H.: "Crystallization and preliminary X-ray crystallographic studies of bovine heart mitochondrial cytochrome bc_1 complex." J.Mol.Biol.221. 379-382 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshikawa, S., Shinzawa-Itoh, K., Ueda, H., Tsukihara, T., Fukumoto, Y., Kubota, T., Kawamoto, M., Fukuyama, K., and Matsubara, H.: "Strategies for crystallization of large membrane protein complexes." J.Cryst.Growth. 122. 298-302 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harada, Y., Sakamoto, T., Shimomura, T., Takamoto, K., Senda, T.and Tsuda, M.: "Total Synthesis of a Gene for Octopus Rhodopsin and Its Preliminary Expression" J.Biochem.110. 501-507 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Deng, H., Manor, D., Weng, G., Rath, P., Koutalos, Y., Ebrey, T., Gebhard, R., Lugtenburg, J., Tsuda, M.and Callender, H.: "Resonance Raman Studies of the HOOP Mode in Octopus Bathorhodopsin with Deutarium Labeled Retinal Chromophores." Biochemistry. 30. 4495-44502 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagamune, T., Honda, J., Cho, W.D., Kamiya, N., Teratani, Y., Hirata, A., Sasabe, H., and Endo, I.: "Crystallization of a Photosensitive Nitrile Hydratase from Rhodococcus sp.N-771." J.Mol.Biol.220. 221-222 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kouyama, T., Yamamoto, M., Kamiya, N., Iwasaki, H., Ueki, T., and Sakurai, I.: "Polyhedral Assembly of a Membrane Protein in its Three-dimensional Crystal." J.Mol.Biol.236 (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamiya, N., and Iwasaki, H.: "Development of a New Imaging Plate Diffractometer (IPD-WAS) for Time-Resolved X-ray Crystallography in Laboratory" J.Appl.Cryst.(to be submitted). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamane, K., Toyoshima, C., and Nishimura, S.: "Ligandinduced functions of the epidermal growth factor receptor required the positively charged region asymmetrically distributed across plasma membrane." Biochem.Biophys.Res.Commun.184. 1301-1310 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoshia, C., Sasabe, H., and Stokes, D.L.: "Three-dimensional cryo-electron microscopy of the calcium ion pump in the sarcoplamisc reticulum membrane" Nature. 362. 499-501 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoshima, C., Yonekura, K., and Sasabe, H.: "Contrast transfer for the frozen hydrated specimen. II : Amplitude contrast at very low frequencies" Ultramicroscopy. 48. 165-176 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, A., Furuno, T., Toyoshima, C., and Sasabe, H.: "Two-dimensional crystallization of catalase on a monolayr film of poly (1-benzyl-L-histidine) spread at the air/water interface." Biochim.Biophys.Acta. 1162. 54-60 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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