1991 Fiscal Year Annual Research Report

進化的分子工学に基づく生体高分子の設計

Research Project

Project/Area Number	03353011
Research Institution	Tokyo Institute of Technology
Principal Investigator	大島泰郎東京工業大学, 生命理工学部, 教授 (60167301)
Co-Investigator(Kenkyū-buntansha)	今中忠行大阪大学, 工学部, 教授 (30029219) 岡田弘輔熊本工業大学, 工学部, 教授 (20028947) 伏見譲埼玉大学, 工学部, 教授 (80011641) 郷通子名古屋大学, 理学部, 教授 (70037290) 柳川弘志三菱化成, 生命科学研究所, 主任研究員
Keywords	分子進化工学 / エクソンシャフリング / タンパク質工学 / タンパク質分子設計 / 進化の分子機構
Research Abstract	進化分子工学の推進について、以下の3つの点について検討した。その結果得られた結論と提言に基づいて、重点領域研究計画を立案し、平成5年度からの研究課題として提案した。 1研究の現状分析:進化分子工学がこれまでに挙げてきた研究成果について検討し、カバ-すべき研究課題について討論した。特に、研究課題を階層別に分けることにより研究の戦略目標と有効な研究手法の組合せを整理することが出来た。 2わが国における研究状況:分担者全員による研究報告を行い、わが国のこの分野の研究活動の特徴、弱点について意見交換を行った。 3早急に推進すべき課題:わが国の研究の特徴を生かし、進化分子工学のより効率的な推捗を計かるにはどの様な課題を緊急、かつ集注的に研究すべきかを討論した。その結果、「空間配列上の適応歩行」という概念を核として、自然選択実験系としては核酸レベル、核タンパク質レベル、細胞レベルの三つの階層について、変異の方法としては点突然変異とエクソンシャッフリングの二つの機構について集中的に研究することが必要と結論した。わが国の研究活動をレベルアップするために必要な研究チ-ムを策定した。進化分子工学の分野により多くの若手研究者が参入するよう公開講演会「進化的分子工学こと始め」を開催し、この分野の将来展望について述べた。

Research Products

(20 results)

All Other

All Publications (20 results)

[Publications] Onodera,k.: "Crystallization and Preliminary X-ray Studies of Chimerie 3-Isoropylmalate Dehydrogenase between Bacillus subtilis and Thermus thermophilus HB8." J.Biochem.109. 1-2 (1991)
[Publications] Kirino,H.: "Molecular cloning and nucleotide sequence of 3-isopropylmalate dehydrogenase gene (leuB) from an extreme thermophile,Thermus aquaticus YT-1." J.Biochem.109. 852-857 (1991)
[Publications] Xu,X.: "Necleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile,Bacillus sp.strain YN-1." J.Biochem.109. 678-683 (1991)
[Publications] Fujiyama,K.: "Characterization of Hybrid Enzymes between 6-Aminohexanoate-Dimer Hydrolase and Its Analogous Protein." J.Fermet.Bioeng.71. 298-302 (1991)
[Publications] Imanaka,T.: "Design of temperature-sensitive penicillinase repressors by replacement of Pro in predicted β-turn structures." J.Bacteriol.174. (1992)
[Publications] Sakada.H.: "Cloning and sequencing of the gene coding for alcohol dehydrogenase of Bacillus stearothermophilus and rational shift of the optimum pH." J.Bacteriol.174. (1992)
[Publications] Yoshino,K.: "Temperature Sweep Gel Electrophoresis:a simple Method to Detect Point Mutations." Nucleic Acids Res.19. 3153 (1991)
[Publications] 伏見譲: "「進化分子工学の始まり」(総説)" 生物物理. 32. 22-25 (1992)
[Publications] Yomo,T.: "Promenade in the sequence space of bacterial catalase by random mutagenesis." Ann.N.Y.Acad.Sci.(1992)
[Publications] Yomo,T.: "No stop codons in the antisense strands of the genes for nylon oligomer degradation." Proc.Natl.Acad.Sci.USA. (1992)
[Publications] Kaneko,K.: "Homeochaos:Dynamic Stability of Symbiotic Network with Population Dynamics and Evolving Mutatioin Rates." Physica D.(1992)
[Publications] Shirai,T.: "RNase-like domain in DNA-directed RNA polymerase II." Proc.Natl.Acad.Sci.USA. 88. 9056-9060 (1991)
[Publications] Takai,Y.: "Evolutionary Origin of Thyroglobulin by Duplication of Esterase Gene." FEBS Lett.282. 17-22 (1991)
[Publications] Tachibana,H.: "Construction of and 'ATG-TAG vector' and direct expression of chicken lysozyme structural domains in Escherichia coli." Gene. Submitted
[Publications] Yanagawa,H.: "A novel minimum ribozyme with oxidoreduction activity." Biochemistry. 29. 10585-10589 (1990)
[Publications] Yanagawa,H.: "Redox nucleosides in Torula yeast RNA:Isolation and Characterization of 5-hydroxyuridine,8-hydroxyguanosine,and 8-hydroxyadenosine." J.Biol.Chem.(1992)
[Publications] Saitoh,E.: "The human cystation gene family:Cloning of three members and evolutionary relationship between cystatins and Bowman Birk type proteinase inhibitors." Biomed,Biochim.Acta.50. 4-6 (1991)
[Publications] Sugimoto.N.: "Stabilization energy of the catalytic core of the Tetrahymena ribozyme." Chem.Lett.345-348 (1991)
[Publications] Oshima,T.: "Early Biochmical Evolution:Speculations on the Biochemistry of Primitive Life;in Evolution of Life:Fossils,Molecles,and Culture (S.Osawa and T.Honjo,eds.)" Springer Verlag,(Tokyo,Berlin,Heiderberg,New York,London,Paris,Hong-Kong,Barcelna), 460 (1991)
[Publications] Nosoh,Y.: "Protein stability and stabilization through protein engineering." Ellis Horwood,London, 223 (1991)

1991 Fiscal Year Annual Research Report

進化的分子工学に基づく生体高分子の設計

Principal Investigator

大島 泰郎 東京工業大学, 生命理工学部, 教授 (60167301)

Research Products

[Publications] Onodera,k.: "Crystallization and Preliminary X-ray Studies of Chimerie 3-Isoropylmalate Dehydrogenase between Bacillus subtilis and Thermus thermophilus HB8." J.Biochem.109. 1-2 (1991)

[Publications] Kirino,H.: "Molecular cloning and nucleotide sequence of 3-isopropylmalate dehydrogenase gene (leuB) from an extreme thermophile,Thermus aquaticus YT-1." J.Biochem.109. 852-857 (1991)

[Publications] Xu,X.: "Necleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile,Bacillus sp.strain YN-1." J.Biochem.109. 678-683 (1991)

[Publications] Fujiyama,K.: "Characterization of Hybrid Enzymes between 6-Aminohexanoate-Dimer Hydrolase and Its Analogous Protein." J.Fermet.Bioeng.71. 298-302 (1991)

[Publications] Imanaka,T.: "Design of temperature-sensitive penicillinase repressors by replacement of Pro in predicted β-turn structures." J.Bacteriol.174. (1992)

[Publications] Sakada.H.: "Cloning and sequencing of the gene coding for alcohol dehydrogenase of Bacillus stearothermophilus and rational shift of the optimum pH." J.Bacteriol.174. (1992)

[Publications] Yoshino,K.: "Temperature Sweep Gel Electrophoresis:a simple Method to Detect Point Mutations." Nucleic Acids Res.19. 3153 (1991)

[Publications] 伏見 譲: "「進化分子工学の始まり」(総説)" 生物物理. 32. 22-25 (1992)

[Publications] Yomo,T.: "Promenade in the sequence space of bacterial catalase by random mutagenesis." Ann.N.Y.Acad.Sci.(1992)

[Publications] Yomo,T.: "No stop codons in the antisense strands of the genes for nylon oligomer degradation." Proc.Natl.Acad.Sci.USA. (1992)

[Publications] Kaneko,K.: "Homeochaos:Dynamic Stability of Symbiotic Network with Population Dynamics and Evolving Mutatioin Rates." Physica D.(1992)

[Publications] Shirai,T.: "RNase-like domain in DNA-directed RNA polymerase II." Proc.Natl.Acad.Sci.USA. 88. 9056-9060 (1991)

[Publications] Takai,Y.: "Evolutionary Origin of Thyroglobulin by Duplication of Esterase Gene." FEBS Lett.282. 17-22 (1991)

[Publications] Tachibana,H.: "Construction of and 'ATG-TAG vector' and direct expression of chicken lysozyme structural domains in Escherichia coli." Gene. Submitted

[Publications] Yanagawa,H.: "A novel minimum ribozyme with oxidoreduction activity." Biochemistry. 29. 10585-10589 (1990)

[Publications] Yanagawa,H.: "Redox nucleosides in Torula yeast RNA:Isolation and Characterization of 5-hydroxyuridine,8-hydroxyguanosine,and 8-hydroxyadenosine." J.Biol.Chem.(1992)

[Publications] Saitoh,E.: "The human cystation gene family:Cloning of three members and evolutionary relationship between cystatins and Bowman Birk type proteinase inhibitors." Biomed,Biochim.Acta.50. 4-6 (1991)

[Publications] Sugimoto.N.: "Stabilization energy of the catalytic core of the Tetrahymena ribozyme." Chem.Lett.345-348 (1991)

[Publications] Oshima,T.: "Early Biochmical Evolution:Speculations on the Biochemistry of Primitive Life;in Evolution of Life:Fossils,Molecles,and Culture (S.Osawa and T.Honjo,eds.)" Springer Verlag,(Tokyo,Berlin,Heiderberg,New York,London,Paris,Hong-Kong,Barcelna), 460 (1991)

[Publications] Nosoh,Y.: "Protein stability and stabilization through protein engineering." Ellis Horwood,London, 223 (1991)

大島泰郎東京工業大学, 生命理工学部, 教授 (60167301)

[Publications] 伏見譲: "「進化分子工学の始まり」(総説)" 生物物理. 32. 22-25 (1992)