細胞膜から核へのインフォメ-ションフロ-に関する総合的研究

1991 Fiscal Year Annual Research Report

• Project/Area Number: 03354027
• Research Institution: Kyushu University
• Principal Investigator: 桐野 豊 九州大学, 薬学部, 教授 (10012668)
• Co-Investigator(Kenkyū-buntansha): 木下 一彦 慶応義塾大学, 理工学部, 教授 (30124366) ; 中西 守 名古屋市立大学, 薬学部, 教授 (90090472) ; 久場 健司 佐賀医科大学, 医学部, 教授 (60080561) ; 吉岡 亨 早稲田大学, 人間科学部, 教授 (70046027) ; 平 則夫 東北大学, 医学部, 教授 (60004553)
• Keywords: 細胞膜 / 核 / インフォメ-ションフロ- / 光学顕微鏡技術 / イメ-ジング生理学 / 電気生理学 / 細胞内情報伝達 / 蛋白質リン酸化

Research Abstract

重点領域研究の発足に向けて、「細胞膜から核へのイフォメ-ションフロ-の機構」に関連する分野の調査研究を分担して行った。そして、細胞膜から核へのインフォメ-ションフロ-の過程の全てを取り上げるのでは、対象が広すぎて焦点がぼけてしまう恐れが強いことから、特にどのような領域を重点的に取り上げるか、種々の方面から検討した。
第1回班会議を、平成3年9月26ー28日に、仙台第一ワシントンホテルと東北大学教養部において行った。この間、桐野豊、木下一彦及び吉岡亨がオ-ガナイザ-となって、生物物理学会のオ-ガナイズド・セッション「細胞内情報伝達研究における新技術:光学顕微鏡イメ-ジングと極微マニピュレ-ション」を開催し、光学顕微鏡技術の現状を総括し、将来への展望を論じた。仙台第一ワシントンホテルにおける討論会には15名の班員が集合して、これまでの調査研究の結果について議論した。その結果、細胞内インフォメ-ションフロ-のうちで、特に重要な課題は、「細胞機能調節におけるイオンチャネルの修飾の役割とその機構」であり、注目すべき研究手法は、高性能光学顕微鏡と電気生理学手法の複合技術であるとの一応の結論を得た。
第2回班会議を平成3年11月20ー23日に、博多サンヒルズホテルと九州大学薬学部において行った。この間、ケンブリッジ大学のDr.Amosを含む新しい光学顕微鏡技術の第一人者を招いて研究会をもち、その後班員の間で調査研究・討論を重ねた。そして、イオンチャネルの中でもカリウムチャネルを重点的に取り上げるべきことが浮き彫りにされてきた。
以上の結果に基づき、代表者の桐野豊が中心となって「カリウムチャネルの構造ー機能相関:単一分子の生理学」と題する重点領域申請書を作製し、2月末に文部省に提出した。

Research Products

• [Publications] Yutaka KIRINO: "Patch-clamp measurements of ion chennels in biomembrane vesicles reconstituted into giant proteoliposomes by freeze-thawing without use of detergent" Methods Neurosci.4. 374-383 (1991)
• [Publications] Kazuo KATOH: "Electron microscopic visualization of actin filaments in the early embryo of drosophila melanogaster:the use of phalloidin and tropomysin" J.Electron Microsc.40. 70-75 (1991)
• [Publications] Tohru MURAKAMI: "Stress fibers in situ in proximal tubules of the rat kidney" Cell Structure and function. 16. 231-240 (1991)
• [Publications] Yumiko KANO: "The distribution and arrangement of microtubules in mammalian skeletal muscle fibers" Cell Structure and Function. 16. 251-261 (1991)
• [Publications] Tour IDE: "Purification of a K^+-channel protein of sarcoplasmic reticulum by assaying the channel activity in the planar lipid bilayer system" Biochim.Biophys.Acta. 1067. 213-220 (1991)
• [Publications] Toru IDE: "Purification of a C1^--channel protein of sarcoplasmic reticulum by assaying the channel activity in the planar lipid bilayer system" Biochem.Biophys.Res.Commun.176. 38-44 (1991)
• [Publications] 葛西 道生: "筋収縮の制御に関連したイオンチャネル" 麻酔と蘇生. 27. 153-161 (1991)
• [Publications] Emiko TAWARA: "Glucose transport of haloferax volcanii requires the Na^+-electrochemical potential gradient and inhibitors for the mammaling gluose transporter inhibit the transport" Biochim.Biophys.Acta. 1070. 293-299 (1991)
• [Publications] Y.IMAMOTO: "Photoreaction cycle of phoborhodopsin studied by low-temperature spectro-photometry" Biochemistry. 30. 7416-7424 (1991)
• [Publications] Kazuhiko KINOSITA,Jr.: "Dual-view microscopy with a single camera:Real-time imaging of molecular orientations and calcium" J.Cell Biol.115. 67-73 (1991)
• [Publications] Hiroyuki HYUGA: "Transient and steady-state deformations of a vesicle with an insulating membrane in response to step-function or alternating electric fields" Jap.J.Appl.Phys.30. 2649-2656 (1991)
• [Publications] S.MINOTA: "Long-term potentiation induced by a sustained rise in the intraterminal Ca^<2+> in bull-frog sympathetic ganglia" J.Physiol.435. 421-438 (1991)
• [Publications] Mitsuo NOHMI: "Ultraviolet light activates blocking actions of dantrolene on intracellular Ca^<2+> release in bullfrog sympathetic neurones" J.Biol.Chem.266. 22254-22259 (1991)
• [Publications] Kenji KUBA: "Spatial and dynamic changes in intracellular Ca^<2+> measured by confocal laser-scanning microscopy in bullfrog sympathetic ganglion cells" Neurosci.Res.10. 245-259 (1991)
• [Publications] Hiroshi Hirai: "Inhibitory action of phospholipid-interacting drugs on transcription initiation in a unclear extract of ehrlich ascites tumor cells" Biochim.Biophys.Acta. 1088. 191-196 (1991)
• [Publications] Hiroshi Hirai: "Binding of simian virus 40 large tumor antigen to phospholipid vesicles" Biochim.Biophys.Acta. 1090. 305-310 (1991)
• [Publications] Akio KANAI: "Heterogeneity and tissue-specific expression of eukaryotic transcription factor S-II-related protein mRNA" J.Biochem.109. 674-677 (1991)
• [Publications] 関水 和久: "DNA複製の開始段階におけるリン脂質による制御の可能性" 蛋白質核酸酸素. 36. 589-594 (1991)
• [Publications] Hiroaki YAMADA: "Comparison of the effects of the novel vasodilator FK409 with those of nitroglycerin in isolated coronary artery of the dog" Br.J.Pharmacol.103. 1713-1718 (1991)
• [Publications] Yuji OKADA: "An analysis of the nitrate-like and K channel opening actions of KRN2391 in canine coronary arterial smooth musle" Br.J.Pharmacol.104. 829-838 (1991)
• [Publications] Keisuke SATOH: "The group at C2 of N-ethylnicotinamide determines the vasodilator potencies and mechanisms of action of nicorandil and its congeners in canine coronary arteries" Naunyn-Schmiedeberg's Arch.Pharmacol.344. 589-595 (1991)
• [Publications] Tatsuro YAMASHITA: "Sialyl cholesterol is translocated into cell nuclei and it promotes neurite outgrowth in a mouse neuroblastoma cell line" Glycobiology. 1. 149-154 (1991)
• [Publications] Hideki YAMAMOTO: "Establishment of a monoclonal antibody directed to the minor novel gangliosides in bovine brain and culturd neural cell lines" Brain Res.558. 117-119 (1991)
• [Publications] Yoko OHASHI: "Fast-atom-bombardment chemistry of sulfatide (3-sulfo-galactosylceramide)" Carbohydrate Res.221. 235-243 (1991)
• [Publications] Hiroshi YAMADA: "Antigen receptor-mediated calcium signals in B cells as revealed by confocal fluoresence microscopy" FEBS Letters. 284. 249-251 (1991)
• [Publications] Nobuyuki OHYAMA: "Membrane fluidity and lipid hapten structure of liposomes affect calcium signals in antigen-specific B cells" Biochemistry. 30. 11154-11156 (1991)
• [Publications] Teruaki HAMANO: "Direct interaction between an antigen-specific B cell clone and an MHC class II-reactive helper T cell clone" J.Leukocyte Biol.
• [Publications] Tomio TADA: "Suppression of immune responses by cloned T cells and their products" Behring Inst.Mitt.
• [Publications] Ken KARASAWA: "Antibodies to synthetic platelet-activating factor (1-o-alkyl-2-o-acetyl-sn-glycero-3-phosphocholine) analogues with substituents at the sn-2 position" J.Biochem.110. 683-687 (1991)
• [Publications] Ken KARASAWA: "Specific binding of antibodies to platelet-activating factor (PAF) as demonstrated by thin-layer chromatography/immunostaining" Lipids. 26. 1122-1125 (1991)
• [Publications] Ken KARASAWA: "Radioimmunoassay for platelet-activating factor" Lipids. 26. 1126-1129 (1991)
• [Publications] Ken KARASAWA: "Lysophospholipase L from escherichia coli K-12 overproducer" J.Biochem.109. 288-293 (1991)
• [Publications] Tasuku HONJO: "Shared partners in receptors" Current Biology. 1. 201-203 (1991)
• [Publications] Yuji SAITO: "Biochemical evidence for a third chain of the interleukin-2 receptor" J.Biol.Chem.266. 22186-22191 (1991)
• [Publications] Hisataka SABE: "Comparison of protein tyrosine phosphorylation and morphological changes induced by IL-2 and IL-3" International Immunology. 3. 1137-1148 (1991)
• [Publications] Yuji YAMANASHI: "Association of B cell antigen receptor with protein tyrosine kinase lyn" Science. 251. 192-194 (1991)
• [Publications] Yuji YAMANASHI: "Differential responses of p56^<lun> and p53^<lun>,products of alternatively spliced lyn mRNA,on stimulation of B-cell antigen receptor" Cell Regulation. 2. 979-987 (1991)
• [Publications] Junichiro MIZUGUCHI: "Tyrosine protein kinase is involved in anti-IgM-mediated signaling in BAL17 B lymphoma cells" J.Immunol.
• [Publications] 水口 純一郎: "Igレセプタ-結合チロシンキナ-ゼ" Medical Immunology. 22. 65-72 (1991)
• [Publications] 中西 守: "細胞活性化とカルシウムイメ-ジングー核へのカルシウムシグナルー" Medical Immunology. 22. 6-9 (1991)
• [Publications] 安西 和紀: "骨格筋小胞体Caポンプの起電性:新しい実験法による研究の新展開" 実験医学.
• [Publications] Kazunori ANZAI: "Formation of ion channels in planar lipid bilayer membranes by synthetic basic peptides" Biochim.Biophys.Acta. 1064. 256-266 (1991)
• [Publications] Naohide HIRASHIMA: "Comparative electrophysiological study of reconstituted membrane preparations of the potassium channel of the rabbit skeletal muscle sarsoplasmic reticulum" Biochim.Biophys.Acta. 1067. 235-240 (1991)
• [Publications] Yukio AGAWA: "Interaction with phospholipid bilayers,ion channel formation,and antimicrobial activity of basic amphipathic α-helical model peptide having various chain length" J.Biol.Chem.266. 20218-20222 (1991)
• [Publications] K.KINOSITA,M.HIBINO,H.ITO,M.SHIGEMORI,K.HIRANO,Y.KIRINO,T.HAYAKAWA(D.C.Chang,B.M.Chassy,J.A.Saunders,E.E.Sowers,Eds.): "Handbook of electroporation and electrofusion" Academic Press, 581 (1991)
• [Publications] 桐野 豊,小沢 俊彦: "エッセンスESRー基礎と実験ー" 廣川書店, 169 (1991)
• [Publications] 加茂 直樹(須貝 新太郎編): "「生物物理工学」" アイビ-シ-, 251 (1991)
• [Publications] 宝谷 紘一,木下 一彦: "限界を超える生物顕微鏡ー見えないものを見るー" 学会出版センタ-, 177 (1991)
• [Publications] 柳澤 輝行,平 則夫(浦川 紀元,唐木 英明編): "カルシウムと情報伝達系ー平滑筋研究の現状と実験法" 文永堂, (1991)