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1994 Fiscal Year Final Research Report Summary

Experimental investigation on the cleft lip and palate Research for preventive factor of this disease using spontaneous cleft lip and palate mice strain

Research Project

Project/Area Number 03404059
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionAichi Gakuin University

Principal Investigator

KAWAI Tsuyoshi  SECOND DEPARTMENT OF ORAL-MAXILLOFACIAL SURGERY,SCHOOL OF DENTISTRY,AICHI-GAKUIN UNIVERSITY ; PROFESSOR, 歯学部・口腔外科学第2講座, 教授 (50064788)

Co-Investigator(Kenkyū-buntansha) TAMURA Itsuko  FUJITA HEALTH UNIVERSITY ; ASSISTANT PROFESSOR, 内科学教室, 研究員
KINOSHITA Hitoyuki  SECOND DEPARTMENT OF ORAL-MAXILLOFACIAL SURGERY,SCHOOL OF DENTISRY,AICHI-GAKUIN,, 歯学部・口腔外科学第2講座, 助手 (70261005)
MIYACHI Hitoshi  SECOND DEPARTMENT OF ORAL-MAXILLOFACIAL SURGERY,SCHOOL OF DENTISRY,AICHI-GAKUIN,, 歯学部・口腔外科学第2講座, 助手 (60261004)
NATSUME Nagato  SECOND DEPARTMENT OF ORAL-MAXILLOFACIAL SURGERY,SCHOOL OF DENTISRY,AICHI-GAKUIN,, 歯学部・口腔外科学第2講座, 講師 (90183532)
Project Period (FY) 1991 – 1994
KeywordsCleft lip / Cleft palate / Preventive factor for cleft palate / Spontaneous cleft lip and palatemice / Estradiol / Congenital anomaly / The transplanted fertilized ova / Neural crest cells
Research Abstract

We performed the following experiment according to our study design :
(1) We collected ova from spontaneous cleft lip and palate A/J mice speciments, transplanted the fertilized ova to C57BL/6 mice specimens said to be genetically free from cleft lip and palate, and studied the development of cleft lip and palate and other external malformations. The embryos obtained showed neither cleft lip and palate nor other external malformations. As a control, fertilized ova of C57BL/6 mice were prepared and transplanted to A/J mice and confirmed the development of cleft and palate. The embryos were bred to check for transmission to the second generation. Results confirmed existence of cleft lip and palate in the second generation.
(2) The total embryo culture method was used to culture embryos of A/J mice with spontaneous cleft lip and palate, and study the development mechanism of cleft lip and palate. The inhibitory effect of estradiol was also examined. The total embryo culture method, however, shortened the culture period, and palate assimilation could not be recognized within the limited observation period. We therefore adopted the organ culture method to study palate assimilation in vitro, and confirmed the preventive effect of estradiol.
(3) Having noted that estradiol therapy was effective in preventing cleft lip and palate in A/J mice, we hypothesized that estradiol promted neural crest cell movement. An experiment was carried out to examine the preventive effects of estradiol on cardiovascular system and thymus malformations originating in neural crest cells. Although in the groups of mice which were administered estradiol on the 8th and 9th day of pregnancy incidences of cleft lip and palate and malformation significantly decreased, no signficant difference in incidence of cardiovascular system malformation was observed. The mice which were administered estradiol on the 7th day of pregnancy showed a lower incidence of cardiovascular system malformation.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Kuniaki Miyajima,Nagato Natsume Hiroo Furukawa,Yoshinari Tanaka Noritaka Tsunoda,Yasushi Morikawa Yutaka Ito,Tetso Iizuka,Tsuyoshi Kawai: "Cephalometric Standards of ICR,C3H,C57BL and A/Jax Strains of Mice" Journal of Growth. 30. 193-196 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masanori Honda,Nagato Natsume,Hiroo Masuda,Tsuyoshi Kawai: "An Observation of the Dental Anlage Based on Findings in Cleft Lip Patients" Plastic and Reconstructive Surgery. 92(7). 1419- (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagato Natsume,Kuniaki Miyajima,Hiroyuki Kinoshita Tsuyoshi Kawai: "Incidence of Cleft Lip and Palate in Beagles" Plastic and Reconstructive Surgery. 93(2). 439- (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 夏目長門,河合幹: "歯科とかかわりのある症候群あれこれ" デンタルダイヤモンド. 17(15). 72-75 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木慎一,宮島邦彰,夏目長門,山田晃弘,田中良成,角田典隆,河合幹,飯塚哲夫: "中型動物のセファログラムの撮影に用いる頭部固定装置の考案" 愛知学院大学歯学会誌. 30(4). 795-800 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河合幹,夏目長門: "口唇口蓋裂発生の機序に関する最近の進歩" 朝日出版社, 202 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 愛知県歯科医師会編: "口腔ケア-" 朝日出版社, 270 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tetsuya Narukawa: "Staphylococcus Aureus Inflammation And Cleft Palate In Mice" Plastic And Reconstructive Surgery. 85 (4). 637-638 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuyoshi Kawai: "Analysis of Facial Deformity in Infants with Cleft Lip and Palate Using Moire Topographies" Asian Journal of Oral Maxillofacial Surgery. 2. 41-53 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuniaki Miyajima: "Cephalometric Standares of ICR,C3H,C57BL and A/Jax Strains of Mice" Journal of Growth. 30. 193-196 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masanori Honda: "An Observation of the Dntal Anlage Based of Findings in Cleft Lip Patients" Plastic and Reconstructive Surgery. 92 (7). 1419 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagato Natsume: "Incidence of cleft lip and palate in beagle." Plastic and Reconstructive Surgery. 93 (2). 439 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagato Natsume: "Lower Jaw Bone Graft of the Alveolar Cleft Region In a Cleft Lip And Palate Patient using the Third-Molar Extraction Procedure" Plastic and Reconstructive Surgery. 93 (6). 1309-1310 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuniaki Miyajima: "Oblique Facial Cleft, Cleft Palate, and Supernumerary Teeth Secondary to Amniotic Bands" Cleft Palate Craniofacial Journal. 31 (6). 483-486 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Murakami: "Prosthetic Treatment of Cleft Lip and Palate Patients Using Rothermann's Attachment" Aichi-Gakuin Dental Science. 7 (1). 41-50 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagato Natsume: "Preference for vegetables Rich in beta-Carotene and Manifestation of Cleft Lip and/or Palate" Plastic and Reconstructive Surgery. 95 (5). 934-935 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaoru Yoshida: "Experimental Study on Cleft Lip and/or Palate : Fetuses of A/J Mice Craniofacial Journalin Uteri of F1 Hybrid Mothers UsingOvarian transplantation" Cleft Palate-Craniofacial Journal. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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