1992 Fiscal Year Final Research Report Summary
Antisense Activities of Ribozymes in in vivo
| Project/Area Number |
03404061
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Faculty of Pharmaceutical Sciences Hokkaido University |
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Principal Investigator |
OHTSUKA Eiko Hokkaido University,Faculty of Pharmaceutical Sciences,Professor, 薬学部, 教授 (80028836)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Hiroyuki Hokkaido University,Faculty of Pharmaceutical Sciences,Instructor, 薬学部, 教務職員 (10204629)
MORIOKA Hiroshi Hokkaido University,Faculty of Pharmaceutical Sciences,Instructor, 薬学部, 助手 (20230097)
INOUE Hideo Hokkaido University,Faculty of PHARMACEUTICAL Sciences,Associate Professor, 薬学部, 助教授 (80088856)
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| Project Period (FY) |
1991 – 1992
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| Keywords | RNA enzyme / Bio-catalysis / ras gene / cell transformation / NIH3T3 cell |
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| Research Abstract |
We have designed hammerhead ribozymes that cleaved the c-Ha-ras mRNA activated at codon 12 (GGU*GUU).Plasmids containing the ribozyme genes were expressed under the control of long terminal repeats of Rous sarcoma virus in NIH3T3 cells transfected with the activated c-Ha-ras gene. These ribozymes were found to inhibit the foci formation (about 50%) by cleavage activities with the ribozyme rather than hybridization to the c-Ha-ras gene product. Further, when the activated c-Ha-ras gene was cotransfected with the ribozyme gene, expression of the activated gene was suppressed by ribozymes in morphologically flat cells.
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