1994 Fiscal Year Annual Research Report

血流に起因する血管内皮細胞反応と血管系構築機序に関する生体工学的研究

Research Project

Project/Area Number	03404062
Research Institution	Institute of Medical Electronics, Faculty of Medicine, Univ.of Tokyo
Principal Investigator	神谷瞭東京大学, 医学部(医), 教授 (50014072)
Co-Investigator(Kenkyū-buntansha)	柴田政廣東京大学, 医学部, 講師 (60158954) 安藤譲二東京大学, 医学部, 客員助教授 (20159528)
Keywords	血管内皮細胞 / ずり応力 / 一酸化窒素 / 接着分子 / リンパ球 / VCAM-1
Research Abstract	当該年度においては主として血管内皮細胞の接着分子の発現に及ぼす流れ負荷の影響について検索を行った。マウスのリンパ節の細静脈内皮細胞に平行平板型の装置で流れずり応力を負荷し接着分子であるVCAM-1、ICAM-1、CD44の蛋白レベルの発現量の変化を各々のモノクローナル抗体を用いたフローサイトメトリーで、mRNAレベルの変化を各々のsense、anti-senseプライマーを用いた逆転写PCR法で解析した。その結果1.5dyn/cm^2のずり応力負荷6時間でVCAM-1の蛋白量は約1/2に減少し、mRNAレベルにも明らかな低下が出現した。一方、CD44は蛋白量が20%程増加し、mRNAレベルにも軽度増加を示した。ICAM-1は蛋白、mRNAとも有意な変化を示さなかった。以上の結果より流れずり応力は白血球との接着に関わる内皮の接着分子の発現を修飾すること、さらにその影響は遺伝子レベルに及んでいることが示された。

Research Products

(7 results)

All Other

All Publications (7 results)

[Publications] J.Ando: "Shear stress inhibits the adhesion of cultured mouse endothelial cells to lymphocytes by down-regulating VCAM-1 expression" Am.J.Physiol.267. C679-C687 (1994)
[Publications] J.Ando: "Intracellular calcium response to directly applied mechanical shearing force in cultured vascular endothelial cells" Biorheology. 31. 57-68 (1994)
[Publications] W.Yang: "Exogenous nitric oxide inhibits proliferation of cultured vascular endothelial cells" Biochem.Biophys.Res.Commun.203. 1160-1167 (1994)
[Publications] R.Korenaga: "Laminar flow stimulates ATP- and shear stress -dependent nitric oxide production in cultured bovine endothelial cells" Biochem.Biophys.Res.Commun.198. 213-219 (1994)
[Publications] A.Kamiya: "Vascular endothelial cell functions and biomechanics" Endothelium. 1. 127-130 (1993)
[Publications] A.Ohtsuka: "The effect of flow on the expression of vascular adhesion molecule-1 by cultured mouse endothelial cells" Biochem.Biophys.Res.Commun.193. 303-310 (1993)
[Publications] A.Kamiya: "Endothelial cell responses to fluid shear stress In “Endothelium-derived factors and vascular functions"" Tomoh Masaki eds.Excerpta Medica,Tokyo, 12 (1994)

1994 Fiscal Year Annual Research Report

血流に起因する血管内皮細胞反応と血管系構築機序に関する生体工学的研究

Principal Investigator

神谷 瞭 東京大学, 医学部(医), 教授 (50014072)

Research Products

[Publications] J.Ando: "Shear stress inhibits the adhesion of cultured mouse endothelial cells to lymphocytes by down-regulating VCAM-1 expression" Am.J.Physiol.267. C679-C687 (1994)

[Publications] J.Ando: "Intracellular calcium response to directly applied mechanical shearing force in cultured vascular endothelial cells" Biorheology. 31. 57-68 (1994)

[Publications] W.Yang: "Exogenous nitric oxide inhibits proliferation of cultured vascular endothelial cells" Biochem.Biophys.Res.Commun.203. 1160-1167 (1994)

[Publications] R.Korenaga: "Laminar flow stimulates ATP- and shear stress -dependent nitric oxide production in cultured bovine endothelial cells" Biochem.Biophys.Res.Commun.198. 213-219 (1994)

[Publications] A.Kamiya: "Vascular endothelial cell functions and biomechanics" Endothelium. 1. 127-130 (1993)

[Publications] A.Ohtsuka: "The effect of flow on the expression of vascular adhesion molecule-1 by cultured mouse endothelial cells" Biochem.Biophys.Res.Commun.193. 303-310 (1993)

[Publications] A.Kamiya: "Endothelial cell responses to fluid shear stress In “Endothelium-derived factors and vascular functions"" Tomoh Masaki eds.Excerpta Medica,Tokyo, 12 (1994)

神谷瞭東京大学, 医学部(医), 教授 (50014072)