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1992 Fiscal Year Final Research Report Summary

Research in Molecular Mechanism of Prolactin Binding to its Receptor

Research Project

Project/Area Number 03454096
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

SAKAI Senkiti  Univ. of TOKYO, Fac. of Agriculture, Associate Professor, 農学部, 助教授 (80114487)

Co-Investigator(Kenkyū-buntansha) AOKI Fugaku  Univ. of Tokyo, Fac. of Agriculture, Assistant Professor, 農学部, 助手 (20175160)
KOHMOTO Kaoru  Univ. of Tokyo, Fac. of Agriculture, Professor, 農学部, 教授 (30011894)
Project Period (FY) 1991 – 1992
Keywordsprolactin / prolactin receptor / plasma membrane receptor / mammary gland / hormone-induced dissociation / negative cooperativity / Scatchard plot / Hill plot
Research Abstract

We have cleared two points in the mammary prolactin (PRL) receptor.
(1) PRL accelerated the dissociation of PRL from its receptor in a dose- dependent manner. Scatchard analysis revealed that this reaction is not a simple bimolecular reaction. The Hill plot was non-linear. At PRL higher than 30 ng/ml, the Hill coeficient changed from 1.0 to 0.7. Using the PRL-preloaded receptor, the receptor remained active for association after PRL-induced dissociation. As PRL accelerated the PRL-binding reaction, it was speculated that PRL at high levels can actively generate a hormonal signal. Negative cooperativity is of much biological importance under conditions where levels of hormone are elevated exceedingly or abnormally.
(2) PRL bound to the cell receptor was dissociated by the treatment with acid in a molarity-dependent manner rather than pH-dependent. The ratio of acid-sensitive to acid-insensitive receptor changed independently after parturiton. By acid treatment of subcellular membranes, about 80% of PRL bound with plasma and intracellular membrane receptors were acid-sensitive and acid-insensitive, respectively. We concluded that plasma membrane PRL receptor is intrinsically acid-sensitive and regulated independently.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] SAKAI Senkiti: "Effect of hormones on dissociation of prolactin from the rabbit mammary gland prolactin recepter" Biochenical Jourael. 279. 461-465 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SUZUKI Maki,KOHMOTO Kaoru,SAKAI Senkiti: "Radioreceptor assay of serum prolachin using nitrocellalose membrane-immobilized mammary paolactin recepter" Analytical Biochemistry. 200. 42-46 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SAKAI Senkiti: "Effect of aclnivistetwin of a cfinomycim D and protein-synthesis inhibitors on the binding of prolactin to the mammey gland recepter" Animal Science and Technology. 63. 1-7 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KANAI-AZUMA,M.,KANAI,Y.,KUROHMARU,M.,SAKAI,S.,HAYASHI,Y.: "Insulin-like growth fador(IGF)-1 stinulates proliferation and migration of mouse ectorlacental conecells, while IGF-II trausforms them into tropho plastic giant cels in uitro" Biology of reproduction. 48. 252-261 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 酒井 仙吉: "プロラクチン受容体による乳腺機能の調節ー受容体遺伝子の構造ー" 日本畜産学会報. 63. 1203-1208 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SAKAI Senkiti: "Effect of hormones on dissociation of prolactin from the rabbit mammary gland prolactin receptor." Biochemical Journal. 279. 461-465 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SUZUKI Maki, KOHMOTO Kaoru, SAKAI Senkiti: "Radioreceptor assay of serum prolactin using nitrocellulose membrane-immobilized mammary prolactin receptor." Analytical Biochemistry. 200. 42-46 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SAKAI Senkiti: "Effect of administration of actinomycin D and protein-synthesis inhibitors on the binding of prolactin to the mammary gland receptor in the ovariectomized mid-pregnant mouse." Animal Science and Technology. 63. 1-7 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KANAI-AZUMA Masami, KANAI Yoshiakira, KUROHMARU Masamichi, SAKAI Senkiti, HAYASHI Yoshihiro: "Insulin-like growth factor (IGF)-I stimulates proliferation and migration of mouse ectoplacental cone cells, while IGF-II transforms them into trophoblastic giant cells in vitro." Biology of Reproduction. 48. 252-261 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SAKAI Senkiti: "Regulation of mammary gland function by prolactin through receptor binding - structure of receptor gene" Animal Science and Technology. 63. 1203-1208 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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