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1992 Fiscal Year Final Research Report Summary

CHARACTERISTIC OF INTRACELLULAR CALCIUM STORES RESPONSIBLE FOR CATECHOLAMINE SECRETION IN ADRENAL MEDULLARY CHROMAFFIN CELLS

Research Project

Project/Area Number 03454103
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

NAKAZATO Yoshikazu  DEPARTMENT OF VETERINARY PHARMACOLOGY, HOKKAIDO UNIVERSITY, 獣医学部, 教授 (60001525)

Co-Investigator(Kenkyū-buntansha) OHTA Toshio  DEPARTMENT OF VETERINARY PHARMACOLOGY, HOKKAIDO UNIVERSITY, 獣医学部, 助手 (20176895)
Project Period (FY) 1991 – 1992
KeywordsCa^<2+> store / Ca^<2+>-induced Ca^<2+> release / Caffeine / Catecholamine / Whole cell patch clamp / Nicotinic currents / Fure 2 / Concentration of intracellular Ca^<2+>
Research Abstract

We have found that unlike catecholamine secretion induced by nicotinic receptor activation, the secretory response to muscarinic receptor stimulation is not simply resulting from increase in Ca^<2+> influx,but from mobilization of intracellulary stored Ca^<2+> in cat adrenal glands (Nakazato et al.,1984). Since the same was true in guinea-pig adrenal glands, Ca^<2+> stores linked to muscarinic receptor have been suggested to be present in the adrenal chromaffin cells, regardless of the species. In this research project, first, the possibility of the presence of Ca^<2+> stores other than those linked to muscarinic receptors was determined by using caffeine which has been known to initiate Ca^<2+>-induced Ca^<2+> release from sarcoplamic reticulum of the muscle. Secondly, the ability of subcellular organella to accumulate ^<45>Ca^<2+> was studied to specify the candidate for Ca^<2+> store, thirdly the effect of caffeine on Ca^<2+> mobilization or catecholamine secretion induced by acetyl … More choline and finally, the effect on membrane currents under the condition of whole cell patch clamp were investigated. The results are summarized in the following; (1) caffeine caused a marked increase in catecholamine secretion from adrenal chromaffin cells of cat, guinea-pig and ox, (2) the effectiveness of caffeine was depressed by the presence of extracellular Ca^<2+>, Mg^<2+> and/or Na^+, (3) ryanodine blocked the secretory response to caffeine, but partially inhibited the response to muscarinic stimulation. (4) Both endoplasmic reticulum and secretory granules had the capacity to accumulate ^<45>Ca^<2+> in a similar extent. (5) Caffeine inhibited catecholamine secretion induced by acetylcholine, high K^+ and veratridine. (6) Acetylcholine initiated nicotinic receptor current and depolarization evoked voltage-dependent Na^+, K^+ and Ca^<2+> in chromaffin cells and (7) all of these currents were inhibited by caffeine. These results suggest that the ineffectiveness of caffeine in releasing catecholamine in the presence of extracellular Ca^<2+> and/or Na^+ is due to the inhibitory action of caffeine itself on both receptor linked and volgate- dependent ionic channels. Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] TERAOKA,H.: "RYANODINE INHIBITS CAFFEINE-EVOKED Ca^<2+> MOBILIZATION AND CATECHOLAMINE SECRETION FROM CULTURED BOVINE ADRENAL CHROMAFFIN CELLS" J.NEURPCHEM. 57. 1884-1890 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SATOH,E.: "EFFECTS OF MONENSIN AND VERATRIDINE ON ACETYLCHOLINE RELEASE AND CYTOSILIC FREE Ca^<2+> LEVELS IN CEREBROCORTICAL SYNAPTOSOMES OF RATS" J.NAUROCHEM. 57. 1270-1275 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SATOH,E.: "MODE OF ACTION OF PALYTOXIN ON THE RELEASE OF ACETYLCHOLINE FROM RAT CEREBROCORTICAL SYNAPTOSOMES" J.NEUROCHEM. 57. 1276-1280 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NAKAZATO,Y.: "COMRARISONS OF THE EFFECT OF RYANODINE ON CATECHOLAMINE SECRETION EVOKED BY CAFFEINE AND ACETYLCHOLINE IN PERFUSED ADRENAL GLANDS OF GUINEA-PIG" BR.J.PHARMACOL. 105. 597-802 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SATOH,E.: "ON THE MECHANISM OF OUANAIN-INDUCED RELEASE OF ACETYLCHOLINE FROM SYNAPTOSOMES" J.NEUROCHEM. 58. 1038-1044 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NAKAZATO,Y.: "INHIBITORY EFFECTS OF CAFFEINE ON AGONIST-INDUCED CATECHOLAMINE SECRETION FROM ADRENAL CHROMAFFIN CELLS" 7TH INTERNATIONAL CATECHOLAMINE SYMPOSIUM,ABSTRACTS. 228 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TERAOKA,H.: "DIFFERENCE IN THE EFFECTIVENESS OF Ca^<2+> EVOKE CATECHOLAMINE SECRETION BETWEEN ADRENALINE AND NORADRENALINE-CONTAINING CELLS OF BOVINE ADRENAL MEDULLA" J.NEUROCHEM. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TERAOKA, H.: "RYANODINE INHIBITS CAFFEINE-EVOKED Ca^<2+> MOBILIZATION AND CATECHOLAMINE SECRETION FROM CULTURED BOVINE ADRENAL CHROMAFFIN CELLS" J. NEUROCHEM.57. 1884-1890 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SATOH, E.: "EFFECTS OF MONENSIN AND VERATRIDINE ON ACETYLCHOLINE RELEASE AND CYTOSOLIC FREE Ca^<2+> LEVELS IN CEREBROCORTICAL SYNAPTOSOMES OF RATS" J. NEUROCHEM.57. 1270-1275 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SATOH, E.: "MODE OF ACTION OF PALYTOXIN ON THE RELEASE OF ACETYLCHOLINE FROM RAT CEREBROCORTICAL SYNAPTOSOMES" J. NEUROCHEM.57. 1276-1280 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAKAZATO, Y.: "COMPARISONS OF THE EFFECT OF RYANODINE ON CATECHOLAMINE SECRETION EVOKED BY CAFFEINE AND ACETYLCHOLINE IN PERFUSED ADRENAL GLANDS OF GUINEA-PIG" BR. J. PHARMACOL.105. 597-802 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SATOH, E.: "ON THE MECHANISM OF OUABAIN-INDUCED RELEASE OF ACETYLCHOLINE FROM SYNAPTOSOMES" J. NEUROCHEM.58. 1038-1044 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAKAZATO, Y.: "INHIBITORY EFFECTS OF CAFFEINE ON AGONIST-INDUCED CATECHOLAMINE SECRETION FROM ADRENAL CHROMAFFIN CELLS" 7TH INTERNATIONAL CATECHOLAMINE SYMPOSIUM, ABSTRACTS. 228 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TERAOKA, H.: "DIFFERENCE IN THE EFFECTIVENESS OF Ca^<2+> EVOKE CATECHOLAMINE SECRETION BETWEEN ADRENALINE- AND NORADRENALINE-CONTAINING CELLS OF BOVINE ADRENAL MEDULLA" J. NEUROCHEM.(1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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