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1993 Fiscal Year Final Research Report Summary

Mechanisms of Neurotransmitter Release in the Central Nervous System

Research Project

Project/Area Number 03454126
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionThe University of Tokyo

Principal Investigator

TACHIBANA Masao  Univ.of Tokyo, Dept.of Psychol.Prof., 文学部, 教授 (60132734)

Project Period (FY) 1991 – 1993
KeywordsSynapse / Neurotransmitter / Glutamate / Ca current / Na / Ca exchanger / Ca pump / Retina / Neuron
Research Abstract

To investigate the excitation-transmitter release coupling in the vertebrate central nervous system, ON-type bipolar cells dissociated from the goldfish retina were used because these cells have an extraordinarily large presynaptic terminal. The Ca current and the intracellular free Ca^<2+> concentration ([Ca^<2+>]_i) were simultaneously monitored. Since the leading candidate for the transmitter is glutamate, the transmitter release was detected electrophysiologically by the neuron, which was highly sensitive to glutamate.
The Ca channel was identified as the high-voltage activated, dihydropyridine-sensitive type, and was highly localized to the presynaptic terminal. The basal level of [Ca^<2+>]_i in the presynaptic terminal was determined by a balance between the leakage influx of Ca^<2+> through Ca^<2+> channels and the extrusion of Ca^<2+> by the plasma membrane Ca^<2+> pump. Activation of the Ca^<2+> current evoked the Ca^<2+> transient. Its peak level seemed to be regulated by endogenous Ca^<2+> buffers. The increased [Ca^<2+>]_i recovered to the basal level due to the extrusion of Ca^<2+> by the Na^+/Ca^<2+> exchanger and the Ca^<2+> pump in the plasma membrane. Activation of the dihydropyridine-sensitive Ca^<2+> current in fact evoked the release of glutamate-like substance.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Tachibana,M: "Release of endogenous excitatory amino acids from ON-type bipolar cells isolated from the goldfish retina." Journal of Neuroscience. 11. 2199-2208 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 立花政夫: "網膜におけるシナプス伝達-双極細胞からの伝達物質の放出-" Vision. 3. 111-120 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tachibana,M.: "Role of dihydropyridine-sensitive calcium current in transmitter release from goldfish bipolar cells." Invest.Optithalmol,Visual Sci.33. 752-752 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tachibana,M.: "Dihydropyridine-sensitive calcium current mediates neurotransmitter release from bipolar cells of the goldfish retina." Journal of Neuroscience. 13. 2898-2909 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tachibana,M.: "Dihydropyridine-sensitive calcium current mediates neurotransmitter release from retinal bipolar cells." Annals of the New York Academy of Sciences. 707. 359-361 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tachibana, M.and Okada, T.: "Release of endogenous excitatory amino acids from ON-type bipolar cells isolated from the goldfish retina" J.Neurosci.11. 2199-2208 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tachibana, M., Okada, T., Arimura, T.and Kobayashi, K.: "Properties, distribution and physiological meaning of calcium channels in on-type bipolar cells of the goldfish retina" Neurosci.Res. 16. S5 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tachibana, M., Okada, T., Arimura, T.and Kobayashi, K.: "Role of dihydropyridine-sensitive calcium current in transmitter release from goldfish bipolar cells" Invest.Ophthal.Visual Sci.33. 752 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tachibana, M., Okada, T., Arimura, T., Kobayashi, K.and Piccolino, M.: "Dihydropyridine-sensitive calcium current mediates neurotransmitter release from bipolar cells of the goldfish retina" J.Neurosci.13. 2898-2909 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tachibana, M., Okada, T., Arimura, T.and Kobayashi, K.: "Dihydropyridine-sensitive calcium current mediates neurotransmitter release from retinal bipolar cells" Annals of the New York Academy of Sciences. 707. 359-361 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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